Staff View: A regenerable screen-printed DNA biosensor based on acrylic microsphere-gold nanoparticle composite for genetically modified soybean determination

A regenerable screen-printed DNA biosensor based on acrylic microsphere-gold nanoparticle composite for genetically modified soybean determination

A regenerable electrochemical DNA biosensor based on a new type of acrylic microspheres and gold nanoparticles (AuNPs) composite coated onto a screen printed electrode (SPE) has been successfully developed for specific determination of the 35 S promoter from cauliflower mosaic virus (CaMV 35S) gene...

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Main Authors:	Alizar, Ulianas,, Lee Yook, Heng,, Tan Ling, Ling,, Musa, Ahmad,, Han-Yih, Lau,, Zamri, Ishak,
Format:	Article
Language:	English
Published:	Elsevier Science Sa 2015
Subjects:	Food GM DNA GM DNA Biosensor Camv 35S Promoter Microspheres
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spelling	my.usim-80182017-02-23T04:52:55Z A regenerable screen-printed DNA biosensor based on acrylic microsphere-gold nanoparticle composite for genetically modified soybean determination Alizar, Ulianas, Lee Yook, Heng, Tan Ling, Ling, Musa, Ahmad, Han-Yih, Lau, Zamri, Ishak, Food GM DNA GM DNA Biosensor Camv 35S Promoter Microspheres A regenerable electrochemical DNA biosensor based on a new type of acrylic microspheres and gold nanoparticles (AuNPs) composite coated onto a screen printed electrode (SPE) has been successfully developed for specific determination of the 35 S promoter from cauliflower mosaic virus (CaMV 35S) gene in soybean. DNA probe was immobilised onto acrylic microspheres via covalent bonding. The presence of modified gene in soybean can be detected via hybridisation of CaMV 35S gene-modified DNA with immobilised DNA probe, which was monitored by differential pulse voltammetry of anthraquinone-2sulfonic acid monohydrate sodium salt (AQMS) as redox indicator during hybridisation event. The peak current signal of AQMS was linearly related to the target CaMV 35S gene concentration over the range of 2 x 10(-15) to 2 x 10(-9) M (R-2 = 0.982) with a very low concentration detect limit (7.79 x 10(-16) M). The recovery test showed satisfactory results of 94.6 +/- 5.1-105.4 +/- 4.9% (n = 5) when the biosensor was used for the determination of genetically modified (GM) DNA sequences extracted from GM soybean samples. The DNA biosensor showed good reproducibility (relative standard deviation (RSD) below 5.0%, n = 5) and regenerability (RSD below 5.0%, n = 7). The biosensor response was stable up to 45 days of storage period at 4 degrees C. The main advantages of this biosensor design are very low detection limit and capability of reusing the biosensor for at least seven times after regeneration with mild sodium hydroxide. (c) 2013 The Authors. Published by Elsevier B. V. All rights reserved. 2015-05-14T06:46:04Z 2015-05-14T06:46:04Z 2014-01-01 Article 0925-4005 en Elsevier Science Sa
institution	Universiti Sains Islam Malaysia
building	USIM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universit Sains Islam i Malaysia
content_source	USIM Institutional Repository
url_provider	http://ddms.usim.edu.my/
language	English
topic	Food GM DNA GM DNA Biosensor Camv 35S Promoter Microspheres
spellingShingle	Food GM DNA GM DNA Biosensor Camv 35S Promoter Microspheres Alizar, Ulianas, Lee Yook, Heng, Tan Ling, Ling, Musa, Ahmad, Han-Yih, Lau, Zamri, Ishak, A regenerable screen-printed DNA biosensor based on acrylic microsphere-gold nanoparticle composite for genetically modified soybean determination
description	A regenerable electrochemical DNA biosensor based on a new type of acrylic microspheres and gold nanoparticles (AuNPs) composite coated onto a screen printed electrode (SPE) has been successfully developed for specific determination of the 35 S promoter from cauliflower mosaic virus (CaMV 35S) gene in soybean. DNA probe was immobilised onto acrylic microspheres via covalent bonding. The presence of modified gene in soybean can be detected via hybridisation of CaMV 35S gene-modified DNA with immobilised DNA probe, which was monitored by differential pulse voltammetry of anthraquinone-2sulfonic acid monohydrate sodium salt (AQMS) as redox indicator during hybridisation event. The peak current signal of AQMS was linearly related to the target CaMV 35S gene concentration over the range of 2 x 10(-15) to 2 x 10(-9) M (R-2 = 0.982) with a very low concentration detect limit (7.79 x 10(-16) M). The recovery test showed satisfactory results of 94.6 +/- 5.1-105.4 +/- 4.9% (n = 5) when the biosensor was used for the determination of genetically modified (GM) DNA sequences extracted from GM soybean samples. The DNA biosensor showed good reproducibility (relative standard deviation (RSD) below 5.0%, n = 5) and regenerability (RSD below 5.0%, n = 7). The biosensor response was stable up to 45 days of storage period at 4 degrees C. The main advantages of this biosensor design are very low detection limit and capability of reusing the biosensor for at least seven times after regeneration with mild sodium hydroxide. (c) 2013 The Authors. Published by Elsevier B. V. All rights reserved.
format	Article
author	Alizar, Ulianas, Lee Yook, Heng, Tan Ling, Ling, Musa, Ahmad, Han-Yih, Lau, Zamri, Ishak,
author_facet	Alizar, Ulianas, Lee Yook, Heng, Tan Ling, Ling, Musa, Ahmad, Han-Yih, Lau, Zamri, Ishak,
author_sort	Alizar, Ulianas,
title	A regenerable screen-printed DNA biosensor based on acrylic microsphere-gold nanoparticle composite for genetically modified soybean determination
title_short	A regenerable screen-printed DNA biosensor based on acrylic microsphere-gold nanoparticle composite for genetically modified soybean determination
title_full	A regenerable screen-printed DNA biosensor based on acrylic microsphere-gold nanoparticle composite for genetically modified soybean determination
title_fullStr	A regenerable screen-printed DNA biosensor based on acrylic microsphere-gold nanoparticle composite for genetically modified soybean determination
title_full_unstemmed	A regenerable screen-printed DNA biosensor based on acrylic microsphere-gold nanoparticle composite for genetically modified soybean determination
title_sort	regenerable screen-printed dna biosensor based on acrylic microsphere-gold nanoparticle composite for genetically modified soybean determination
publisher	Elsevier Science Sa
publishDate	2015
_version_	1645152322573041664
score	13.222552

A regenerable screen-printed DNA biosensor based on acrylic microsphere-gold nanoparticle composite for genetically modified soybean determination

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