Vanillin: Bio-Synthetic Flavour Production from Paddy Straw
Lignocellulosic materials are important natural renewable resources. This is because it is low in cost, renewable and it requires low processing energies. Agricultural residues, such as paddy straw are sources for lignocellulosic materials. Paddy straws are unique in their chemical compositions a...
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| Main Authors: | , , |
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| Format: | Conference Paper |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://ddms.usim.edu.my/handle/123456789/6138 |
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| Summary: | Lignocellulosic materials are important natural renewable resources. This is because it is
low in cost, renewable and it requires low processing energies. Agricultural residues, such
as paddy straw are sources for lignocellulosic materials. Paddy straws are unique in their
chemical compositions as well as their chemical, physical and mechanical properties. It
consisting mainly of cellulose, hemicelluloses, lignin and a small amount of extractives.
Vanillin (3-methoxy-4-hydroxybenzaldehyde) is the major component responsible for the
characteristic of vanilla bean extracts. Due to the high cost of vanilla flavour, bio-synthetic
flavour from lignocellulosic materials has been found to be new potential substrate for
vanillin production rather than extraction from vanilla pod. A number of pretreatment
methods of lignocellulosic materials to release lignin, cellulose and hemicelluloses are
addressed such as chemical and stream pretreatment. Alkaline hydrolysis was chosen as
the most suitable method to release ferulic acid for low lignin content biomass. Optimum
conditions for vanillic acid obtained from alkaline hydrolysis using Sodium Hydroxide,
NaOH of paddy straw at different temperature (80%. 90°C and 100°C) and NaOH
concentration (3M, 4M and 5M) were 4M NaOH, 120°C for 2 hours which yielded 6.56
mglg of ferulic acid. The result obtained showed that lower concentration of NaOH, high
temperature and longer reaction time have favored the ferulic acid formation.
Bioconversion of ferulic acid to vanilic acid using an optimum condition using Aspergillus
niger as the fermentative organism are then being used to vanillin production using
Pycnoporus cinnabarinus. |
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