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Investigating the Insecticide Resistance Mechanisms in a Pyrethroid Resistance Aedes Aegypti Population from Mexico

The mosqirito Aedes ae5vyti is the primary vector for arboviruses such as Dengue, which can gave rises to serious complications such as dengue haemorrhagic fever and dengue shock syndrome which can threatened human life. Since the control of this disease mainly relies on targeting the mosquito ve...

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Main Author: Zarini Ismail
Format: Thesis
Language:English
Published: Universiti Sains Islam Malaysia 2012
Subjects:
Dengue > Prevention & Control
Aedes
tes WC528 Z37i 2009
Insecticides
Insect Vectors
Mexico
Online Access:http://ddms.usim.edu.my/handle/123456789/5684
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spelling my.usim-56842013-02-14T06:58:05Z Investigating the Insecticide Resistance Mechanisms in a Pyrethroid Resistance Aedes Aegypti Population from Mexico Zarini Ismail Dengue--Prevention & Control Aedes tes WC528 Z37i 2009 Insecticides Insect Vectors Mexico The mosqirito Aedes ae5vyti is the primary vector for arboviruses such as Dengue, which can gave rises to serious complications such as dengue haemorrhagic fever and dengue shock syndrome which can threatened human life. Since the control of this disease mainly relies on targeting the mosquito vector by using insecticides, the emergence of resistance to insecticide threatens the success of control programmes and hence increases disease transmission. As there are limited numbers of insecticides used in vector control programmes, monitoring and early detection of insecticide resistance mechanism in mosquito population is a crucial step i~ determined the appropriate resistance management. Here we investigated the resistance mechanisms for both target site and metabolic resistance in pyrethroid resistance Ae. aegypti popillation from Mexico, the Merida strain using a number of molecular techniques. Allele-specific PCR was employed to determine an as yet unpublished novel kdr mutation, whilst the Heated Oligonucleotide Ligation Assay (HOLA) was used to determine the previously reported kdr. mutation Valine 10 16Isoleucine and Isoleucine 10 1 1 Valine. Finally metabolic resistance was assessed using multiplex quantitative PCR to determine whether a selection of cytochrome P450s and Glutathione-S transferases (GSTs) were contributing to the resistance phenotype. Here we reported the association of both target site and metabolic resistance conferring the resistance status to Ae .aegypti Merida. Presented is evidence of a novel kdr. mutation in exon 29, segment 6 domain 111 and in addition to the presence of a kdr mutation ValinelOl6Isoleucine at segment 6 domain 11 of voltage gated sodium channel gene. Furthermore, members of the epsilon GST class (GSTe2, GSTe4 and GSTe7) and members of CYP9 P450 family (CI'P9JZ.l and CYP9J32) were highly expressed. These findings will provide a basis for identifying the resistance associated enzymes within these strain of mosquito and enable resistance status to be monitored and managed appropriately. 2012-07-19T01:46:06Z 2012-07-19T01:46:06Z 2009 Thesis http://ddms.usim.edu.my/handle/123456789/5684 en Universiti Sains Islam Malaysia
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language English
topic Dengue--Prevention & Control
Aedes
tes WC528 Z37i 2009
Insecticides
Insect Vectors
Mexico
spellingShingle Dengue--Prevention & Control
Aedes
tes WC528 Z37i 2009
Insecticides
Insect Vectors
Mexico
Zarini Ismail
Investigating the Insecticide Resistance Mechanisms in a Pyrethroid Resistance Aedes Aegypti Population from Mexico
description The mosqirito Aedes ae5vyti is the primary vector for arboviruses such as Dengue, which can gave rises to serious complications such as dengue haemorrhagic fever and dengue shock syndrome which can threatened human life. Since the control of this disease mainly relies on targeting the mosquito vector by using insecticides, the emergence of resistance to insecticide threatens the success of control programmes and hence increases disease transmission. As there are limited numbers of insecticides used in vector control programmes, monitoring and early detection of insecticide resistance mechanism in mosquito population is a crucial step i~ determined the appropriate resistance management. Here we investigated the resistance mechanisms for both target site and metabolic resistance in pyrethroid resistance Ae. aegypti popillation from Mexico, the Merida strain using a number of molecular techniques. Allele-specific PCR was employed to determine an as yet unpublished novel kdr mutation, whilst the Heated Oligonucleotide Ligation Assay (HOLA) was used to determine the previously reported kdr. mutation Valine 10 16Isoleucine and Isoleucine 10 1 1 Valine. Finally metabolic resistance was assessed using multiplex quantitative PCR to determine whether a selection of cytochrome P450s and Glutathione-S transferases (GSTs) were contributing to the resistance phenotype. Here we reported the association of both target site and metabolic resistance conferring the resistance status to Ae .aegypti Merida. Presented is evidence of a novel kdr. mutation in exon 29, segment 6 domain 111 and in addition to the presence of a kdr mutation ValinelOl6Isoleucine at segment 6 domain 11 of voltage gated sodium channel gene. Furthermore, members of the epsilon GST class (GSTe2, GSTe4 and GSTe7) and members of CYP9 P450 family (CI'P9JZ.l and CYP9J32) were highly expressed. These findings will provide a basis for identifying the resistance associated enzymes within these strain of mosquito and enable resistance status to be monitored and managed appropriately.
format Thesis
author Zarini Ismail
author_facet Zarini Ismail
author_sort Zarini Ismail
title Investigating the Insecticide Resistance Mechanisms in a Pyrethroid Resistance Aedes Aegypti Population from Mexico
title_short Investigating the Insecticide Resistance Mechanisms in a Pyrethroid Resistance Aedes Aegypti Population from Mexico
title_full Investigating the Insecticide Resistance Mechanisms in a Pyrethroid Resistance Aedes Aegypti Population from Mexico
title_fullStr Investigating the Insecticide Resistance Mechanisms in a Pyrethroid Resistance Aedes Aegypti Population from Mexico
title_full_unstemmed Investigating the Insecticide Resistance Mechanisms in a Pyrethroid Resistance Aedes Aegypti Population from Mexico
title_sort investigating the insecticide resistance mechanisms in a pyrethroid resistance aedes aegypti population from mexico
publisher Universiti Sains Islam Malaysia
publishDate 2012
url http://ddms.usim.edu.my/handle/123456789/5684
_version_ 1645151729250992128
score 13.222552

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