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Effect of pH, heat treatment and enzymes on the antifungal activity of lactic acid bacteria against Candida species

The objectives of this study were to evaluate the inhibitory activity of the cell-free supernatants (CFS) of lactic acid bacteria (LAB) isolates and determine the effect of pH, enzymes and heat treatment on the antifungal activity against Candida species. Methodology and results: A total of...

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Main Authors: Bulgasem Youns Bulgasem, Zaiton Hassan, Nur Huda-Faujan, Ramadan Hadia Ali Omar, Mohd Nizam Lani, Mohamed Idris Alshelmani
Format: Article
Language:English
Published: Malaysian Society for Microbiology 2018
Subjects:
Lactic acid bacteria, antifungal activity, pathogenic Candida species
Online Access:http://ddms.usim.edu.my:80/jspui/handle/123456789/16045
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spelling my.usim-160452018-04-27T05:20:06Z Effect of pH, heat treatment and enzymes on the antifungal activity of lactic acid bacteria against Candida species Bulgasem Youns Bulgasem, Zaiton Hassan Nur Huda-Faujan, Ramadan Hadia Ali Omar Mohd Nizam Lani, Mohamed Idris Alshelmani Lactic acid bacteria, antifungal activity, pathogenic Candida species The objectives of this study were to evaluate the inhibitory activity of the cell-free supernatants (CFS) of lactic acid bacteria (LAB) isolates and determine the effect of pH, enzymes and heat treatment on the antifungal activity against Candida species. Methodology and results: A total of 25 strains of LAB were isolated from honey samples from Malaysia, Libya, Saudi Arabia, and Yemen. Four from twenty-five LAB isolates showed antifungal activity against Candida spp. and were identified as Lactobacillus plantarum (HS), L. curvatus (HH), Pediococcus acid lactic (HC), and P. pentosaceus (HM) using 16S rDNA sequence. The CFS of these isolates were evaluated for their antifungal activity using microtiter plate assay. The antifungal activity showed significant inhibitory activity against all Candida spp. especially growth of C. glabrata ATCC 2001 was significant (p < 0.001) completely inhibited by CFS of HH and HM at pH 3. Similarly, growth of C. glabrata ATCC2001 was significantly inhibited (p < 0.001) when treated with previously heated CFS of L. curvatus HH and P. pentosaceus HM at 90 °C and 121 °C. While, the growth of C. krusei ATCC 6258 was completely inhibited by CFS of L. curvatus HH at 121 °C. Treatment the CFS of LAB isolates with proteinase K and RNase II increased the antifungal activity against C. krusei and C. glabrata, whereas the activity of CFS produced by P. acidilactici was lost when treated with RNase II, especially against C. krusei. Conclusion, significance and impact of study: This study demonstrated that treated supernatant of LAB isolates with heating, adjusted pH and enzymes can be used to inhibit the growth of pathogenic Candida spp. 2018-04-27T05:20:06Z 2018-04-27T05:20:06Z 2017 Article 1823-8262 http://ddms.usim.edu.my:80/jspui/handle/123456789/16045 en Malaysian Society for Microbiology
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language English
topic Lactic acid bacteria, antifungal activity, pathogenic Candida species
spellingShingle Lactic acid bacteria, antifungal activity, pathogenic Candida species
Bulgasem Youns Bulgasem, Zaiton Hassan
Nur Huda-Faujan, Ramadan Hadia Ali Omar
Mohd Nizam Lani, Mohamed Idris Alshelmani
Effect of pH, heat treatment and enzymes on the antifungal activity of lactic acid bacteria against Candida species
description The objectives of this study were to evaluate the inhibitory activity of the cell-free supernatants (CFS) of lactic acid bacteria (LAB) isolates and determine the effect of pH, enzymes and heat treatment on the antifungal activity against Candida species. Methodology and results: A total of 25 strains of LAB were isolated from honey samples from Malaysia, Libya, Saudi Arabia, and Yemen. Four from twenty-five LAB isolates showed antifungal activity against Candida spp. and were identified as Lactobacillus plantarum (HS), L. curvatus (HH), Pediococcus acid lactic (HC), and P. pentosaceus (HM) using 16S rDNA sequence. The CFS of these isolates were evaluated for their antifungal activity using microtiter plate assay. The antifungal activity showed significant inhibitory activity against all Candida spp. especially growth of C. glabrata ATCC 2001 was significant (p < 0.001) completely inhibited by CFS of HH and HM at pH 3. Similarly, growth of C. glabrata ATCC2001 was significantly inhibited (p < 0.001) when treated with previously heated CFS of L. curvatus HH and P. pentosaceus HM at 90 °C and 121 °C. While, the growth of C. krusei ATCC 6258 was completely inhibited by CFS of L. curvatus HH at 121 °C. Treatment the CFS of LAB isolates with proteinase K and RNase II increased the antifungal activity against C. krusei and C. glabrata, whereas the activity of CFS produced by P. acidilactici was lost when treated with RNase II, especially against C. krusei. Conclusion, significance and impact of study: This study demonstrated that treated supernatant of LAB isolates with heating, adjusted pH and enzymes can be used to inhibit the growth of pathogenic Candida spp.
format Article
author Bulgasem Youns Bulgasem, Zaiton Hassan
Nur Huda-Faujan, Ramadan Hadia Ali Omar
Mohd Nizam Lani, Mohamed Idris Alshelmani
author_facet Bulgasem Youns Bulgasem, Zaiton Hassan
Nur Huda-Faujan, Ramadan Hadia Ali Omar
Mohd Nizam Lani, Mohamed Idris Alshelmani
author_sort Bulgasem Youns Bulgasem, Zaiton Hassan
title Effect of pH, heat treatment and enzymes on the antifungal activity of lactic acid bacteria against Candida species
title_short Effect of pH, heat treatment and enzymes on the antifungal activity of lactic acid bacteria against Candida species
title_full Effect of pH, heat treatment and enzymes on the antifungal activity of lactic acid bacteria against Candida species
title_fullStr Effect of pH, heat treatment and enzymes on the antifungal activity of lactic acid bacteria against Candida species
title_full_unstemmed Effect of pH, heat treatment and enzymes on the antifungal activity of lactic acid bacteria against Candida species
title_sort effect of ph, heat treatment and enzymes on the antifungal activity of lactic acid bacteria against candida species
publisher Malaysian Society for Microbiology
publishDate 2018
url http://ddms.usim.edu.my:80/jspui/handle/123456789/16045
_version_ 1645154081782628352
score 13.211869

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