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Antifungal Activity of Lactic Acid Bacteria Isolated from Soil Rhizosphere on Fusarium Species Infected Chilli Seeds

Lactic acid bacteria (LAB) are known to inhibit the growth of fungi and LAB cells or its can be used as biocontrol against plant pathogens. The aim of this study was to evaluate the efficacy of selected LAB strains isolated from rhizospheric soil against Fusarium spp. These fungi were isolated from...

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Main Authors: Akaram Husaina, Zaiton Hassan, Nur Huda-Faujan, Mohd Nizam Lani
Format: Article
Language:English
Published: Global Society of Scientific Research and Researchers 2018
Subjects:
Antifungal activity; chilli seeds germination; Fusarium spp; Lactic Acid Bacteria; Rhizospheric soil.
Online Access:http://ddms.usim.edu.my:80/jspui/handle/123456789/16043
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spelling my.usim-160432018-04-27T04:50:01Z Antifungal Activity of Lactic Acid Bacteria Isolated from Soil Rhizosphere on Fusarium Species Infected Chilli Seeds Akaram Husaina, Zaiton Hassan Nur Huda-Faujan, Mohd Nizam Lani Antifungal activity; chilli seeds germination; Fusarium spp; Lactic Acid Bacteria; Rhizospheric soil. Lactic acid bacteria (LAB) are known to inhibit the growth of fungi and LAB cells or its can be used as biocontrol against plant pathogens. The aim of this study was to evaluate the efficacy of selected LAB strains isolated from rhizospheric soil against Fusarium spp. These fungi were isolated from infected different plant parts and identified as F. oxysporum strain KAML01, Fusarium sp. CID124, Fusarium sp. fus 124 and F. proliferatum with 99 % similarity using Internal Transcribed Spacer (ITS) analysis. Five LAB strains were showed strong antifungal activity against the Fusarium spp. were evaluated using dual overlay method. Isolates MSS1 and FCF11 were 100% similarity with Lactobacillus plantarum and isolate MSS5 99% was similarity with Pediococcus pentoceous using 16S rDNA sequence analysis. Inhibition of Fusarium sp. CID124 fungal biomass in the de Man, Rogosa and Sharpe Broth (MRSB) was significantly greater (P ≤ 0.05) by cells of P. pentosaceus (97.43%) compared to inhibition of Fusarium sp. CID124 fungal biomass by Cell Free Supernatant (CFS) of Lb. Acidophilus ATCC314 (91.8%) using biomass dry method in Malt Extract Broth (MEB) medium. Fusarium spp. infected to seeds of three cultivars, Cili Kulai, Red spicy-0639 and GAAC-SEED-5005 and then, treated with CFS-LAB. All treated seeds with CFS-LAB showed good germination in the range of 82.6 to 93.3% compared to seed germination 53.3 to 70.0% of chili seeds infected with Fusarium spp. The percentage germination of chilli seeds was strongly suppressed when infected with Fusarium sp. CID124. This infection had significant (P≤0.05) reduction of seed germination to 53.3 % compared to other Fusarium spp. Finally, the pathogenicity effect of Fusarium sp. CID124-CS was highly virulent on seeds germination of chilli. 2018-04-27T04:50:01Z 2018-04-27T04:50:01Z 2017 Article 2313-4410 http://ddms.usim.edu.my:80/jspui/handle/123456789/16043 en Global Society of Scientific Research and Researchers
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language English
topic Antifungal activity; chilli seeds germination; Fusarium spp; Lactic Acid Bacteria; Rhizospheric soil.
spellingShingle Antifungal activity; chilli seeds germination; Fusarium spp; Lactic Acid Bacteria; Rhizospheric soil.
Akaram Husaina, Zaiton Hassan
Nur Huda-Faujan, Mohd Nizam Lani
Antifungal Activity of Lactic Acid Bacteria Isolated from Soil Rhizosphere on Fusarium Species Infected Chilli Seeds
description Lactic acid bacteria (LAB) are known to inhibit the growth of fungi and LAB cells or its can be used as biocontrol against plant pathogens. The aim of this study was to evaluate the efficacy of selected LAB strains isolated from rhizospheric soil against Fusarium spp. These fungi were isolated from infected different plant parts and identified as F. oxysporum strain KAML01, Fusarium sp. CID124, Fusarium sp. fus 124 and F. proliferatum with 99 % similarity using Internal Transcribed Spacer (ITS) analysis. Five LAB strains were showed strong antifungal activity against the Fusarium spp. were evaluated using dual overlay method. Isolates MSS1 and FCF11 were 100% similarity with Lactobacillus plantarum and isolate MSS5 99% was similarity with Pediococcus pentoceous using 16S rDNA sequence analysis. Inhibition of Fusarium sp. CID124 fungal biomass in the de Man, Rogosa and Sharpe Broth (MRSB) was significantly greater (P ≤ 0.05) by cells of P. pentosaceus (97.43%) compared to inhibition of Fusarium sp. CID124 fungal biomass by Cell Free Supernatant (CFS) of Lb. Acidophilus ATCC314 (91.8%) using biomass dry method in Malt Extract Broth (MEB) medium. Fusarium spp. infected to seeds of three cultivars, Cili Kulai, Red spicy-0639 and GAAC-SEED-5005 and then, treated with CFS-LAB. All treated seeds with CFS-LAB showed good germination in the range of 82.6 to 93.3% compared to seed germination 53.3 to 70.0% of chili seeds infected with Fusarium spp. The percentage germination of chilli seeds was strongly suppressed when infected with Fusarium sp. CID124. This infection had significant (P≤0.05) reduction of seed germination to 53.3 % compared to other Fusarium spp. Finally, the pathogenicity effect of Fusarium sp. CID124-CS was highly virulent on seeds germination of chilli.
format Article
author Akaram Husaina, Zaiton Hassan
Nur Huda-Faujan, Mohd Nizam Lani
author_facet Akaram Husaina, Zaiton Hassan
Nur Huda-Faujan, Mohd Nizam Lani
author_sort Akaram Husaina, Zaiton Hassan
title Antifungal Activity of Lactic Acid Bacteria Isolated from Soil Rhizosphere on Fusarium Species Infected Chilli Seeds
title_short Antifungal Activity of Lactic Acid Bacteria Isolated from Soil Rhizosphere on Fusarium Species Infected Chilli Seeds
title_full Antifungal Activity of Lactic Acid Bacteria Isolated from Soil Rhizosphere on Fusarium Species Infected Chilli Seeds
title_fullStr Antifungal Activity of Lactic Acid Bacteria Isolated from Soil Rhizosphere on Fusarium Species Infected Chilli Seeds
title_full_unstemmed Antifungal Activity of Lactic Acid Bacteria Isolated from Soil Rhizosphere on Fusarium Species Infected Chilli Seeds
title_sort antifungal activity of lactic acid bacteria isolated from soil rhizosphere on fusarium species infected chilli seeds
publisher Global Society of Scientific Research and Researchers
publishDate 2018
url http://ddms.usim.edu.my:80/jspui/handle/123456789/16043
_version_ 1645154081487978496
score 13.222552

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