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Potency Of Honey As Antibacterial Agent Against Multiple Antibiotic Resistant Pathogens Evaluated By Different Methods

Honey is rich with complex natural components which could be useful as antibacterial agents or as preservative. Honey contains high concentration of sugars, low amount of water, high osmolality and often dark colour which influence its antibacterial activity. Disc diffusion, well method, micro dil...

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Main Authors: Mohamed Mustafa Aween, Zaiton Hassan, Nur Huda-Faujani, Mohamed Muftah Emdakim, Belal Jamal Muhialdin
Format: Article
Language:English
Published: Science Publications 2018
Subjects:
Honey, Antibacterial Activity Assay, Multiple Antibiotic Resistant (MAR), Pathogenic Bacteria
Online Access:http://ddms.usim.edu.my:80/jspui/handle/123456789/16035
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spelling my.usim-160352018-04-26T07:14:41Z Potency Of Honey As Antibacterial Agent Against Multiple Antibiotic Resistant Pathogens Evaluated By Different Methods Mohamed Mustafa Aween, Zaiton Hassan Nur Huda-Faujani, Mohamed Muftah Emdakim Belal Jamal Muhialdin Honey, Antibacterial Activity Assay, Multiple Antibiotic Resistant (MAR), Pathogenic Bacteria Honey is rich with complex natural components which could be useful as antibacterial agents or as preservative. Honey contains high concentration of sugars, low amount of water, high osmolality and often dark colour which influence its antibacterial activity. Disc diffusion, well method, micro dilution assay are methods commonly used to determine the antibacterial activity of honey. In this study, microtiter and microbial plate count were included to ascertain the potency of honey as antibacterial agent against multiple antibiotic resistant pathogenic bacteria (Staphylococcus aureus, Salmonella Typhimurium, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa) with concentration of 0.2 g mL−1 . Results found that well diffusion method tends to give higher inhibitory zone than disc diffusion method but there was no correlation among the bacteria was observed except for S. Typhimurium, E. coli (R = 0.310, 0.505 and 0.316, respectively). Nan photometer assay and microtiter plates assay showed comparable results with moderately strong correlation (R2 = 0.681 and 0.767, respectively) for S. aureus and S. typhimurium, but poor correlation was found for E. coli, B. subtilis and P. aeruginosa (R2 = 0.441, 0.308 and 0.383, respectively). Determining the number of survivors by plating on agar after nanophotometer assay or microtiter plate assay had confirmed the effectiveness of honey as antimicrobial agent against target bacteria; which confirmed that honey has the potency to inhibit pathogens even at low concentration. 2018-04-26T07:14:41Z 2018-04-26T07:14:41Z 2014 Article 1546-9239 http://ddms.usim.edu.my:80/jspui/handle/123456789/16035 en Science Publications
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language English
topic Honey, Antibacterial Activity Assay, Multiple Antibiotic Resistant (MAR), Pathogenic Bacteria
spellingShingle Honey, Antibacterial Activity Assay, Multiple Antibiotic Resistant (MAR), Pathogenic Bacteria
Mohamed Mustafa Aween, Zaiton Hassan
Nur Huda-Faujani, Mohamed Muftah Emdakim
Belal Jamal Muhialdin
Potency Of Honey As Antibacterial Agent Against Multiple Antibiotic Resistant Pathogens Evaluated By Different Methods
description Honey is rich with complex natural components which could be useful as antibacterial agents or as preservative. Honey contains high concentration of sugars, low amount of water, high osmolality and often dark colour which influence its antibacterial activity. Disc diffusion, well method, micro dilution assay are methods commonly used to determine the antibacterial activity of honey. In this study, microtiter and microbial plate count were included to ascertain the potency of honey as antibacterial agent against multiple antibiotic resistant pathogenic bacteria (Staphylococcus aureus, Salmonella Typhimurium, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa) with concentration of 0.2 g mL−1 . Results found that well diffusion method tends to give higher inhibitory zone than disc diffusion method but there was no correlation among the bacteria was observed except for S. Typhimurium, E. coli (R = 0.310, 0.505 and 0.316, respectively). Nan photometer assay and microtiter plates assay showed comparable results with moderately strong correlation (R2 = 0.681 and 0.767, respectively) for S. aureus and S. typhimurium, but poor correlation was found for E. coli, B. subtilis and P. aeruginosa (R2 = 0.441, 0.308 and 0.383, respectively). Determining the number of survivors by plating on agar after nanophotometer assay or microtiter plate assay had confirmed the effectiveness of honey as antimicrobial agent against target bacteria; which confirmed that honey has the potency to inhibit pathogens even at low concentration.
format Article
author Mohamed Mustafa Aween, Zaiton Hassan
Nur Huda-Faujani, Mohamed Muftah Emdakim
Belal Jamal Muhialdin
author_facet Mohamed Mustafa Aween, Zaiton Hassan
Nur Huda-Faujani, Mohamed Muftah Emdakim
Belal Jamal Muhialdin
author_sort Mohamed Mustafa Aween, Zaiton Hassan
title Potency Of Honey As Antibacterial Agent Against Multiple Antibiotic Resistant Pathogens Evaluated By Different Methods
title_short Potency Of Honey As Antibacterial Agent Against Multiple Antibiotic Resistant Pathogens Evaluated By Different Methods
title_full Potency Of Honey As Antibacterial Agent Against Multiple Antibiotic Resistant Pathogens Evaluated By Different Methods
title_fullStr Potency Of Honey As Antibacterial Agent Against Multiple Antibiotic Resistant Pathogens Evaluated By Different Methods
title_full_unstemmed Potency Of Honey As Antibacterial Agent Against Multiple Antibiotic Resistant Pathogens Evaluated By Different Methods
title_sort potency of honey as antibacterial agent against multiple antibiotic resistant pathogens evaluated by different methods
publisher Science Publications
publishDate 2018
url http://ddms.usim.edu.my:80/jspui/handle/123456789/16035
_version_ 1645154080023117824
score 13.222552

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