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Isolation, characterization and antibacterial activity of Lactic Acid Bacteria from commercial honey marketed in Malaysia

Thesis (M.Sc)-- Faculty of Science and Technology, Universiti Sains Islam Malaysia, 2012

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Bibliographic Details
Main Author: Mohamed Mustafa Aween
Format: Thesis
Language:English
Published: Universiti Sains Islam Malaysia 2018
Subjects:
Lactic acid bacteria
Honey
Online Access:http://ddms.usim.edu.my:80/jspui/handle/123456789/16017
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spelling my.usim-160172018-05-18T07:11:19Z Isolation, characterization and antibacterial activity of Lactic Acid Bacteria from commercial honey marketed in Malaysia Mohamed Mustafa Aween Lactic acid bacteria Honey Thesis (M.Sc)-- Faculty of Science and Technology, Universiti Sains Islam Malaysia, 2012 A total of 32 lactic acid bacteria (LAB) were isolated from 13 honey samples commercially marketed in Malaysia, and six strains identified as (Lactobacilus acidophilus) by API CHL50 were evaluated for their antibacterial activities against multiple antibiotic resistant (Staphylococcus epidermis), (Bacilus subtilis), (Salmonella typhimurium), (Escherichia coli), (Klebsiella pneumoniae), (Shigella sonnei), (Serretia marcescens) and (Enterobacter aerogenes). Using the overlay agar method the inhibition zone varies with target bacteria which were (S. aureus) 25 to 32 mm, (Staphycoloccus epidermis) 14 to 22 mm, (Bacillus subtilis) 12 to19 mm, (Salmonella typhimurium) 23 to 30 mm, (E. coli) 7 to 18 mm, (Klebsiella pneumoniae) 13.5 to 21.5 mm, (S. sonnei) 9 to 22 mm, (Serretia marcescens) 7.5 to 18 mm, and (E. aerogenes) 10 to 18 mm after 24 h incubation at 30 degree celcius. The effects of temperature, protolithic enzymes and pH on the antibacterial activity of the supernatants were evaluated. The supernatant was stable at 90 degree celcius and 121 degree celcius for 1 hour. Treatment with proteinase K and RNase II maintained the antimicrobial activity of all the supernatants ecxept H006-A from Libyan honey and H010-G from New Zealand honey. All the supernatants showed antimicrobial activities against target bacteria at pH 3 and pH 5 but not pH 6 except against (Enterobacter aerogenes) within 48 h incubation at 30 degree celcius. (Staphylococcus aureus) was not inhibited by H006-A isolated from Libyan honey and H008-d isolated from Malaysian honey at pH 5, compared to supernatants from other (L. acidophilus) isolates. Result from PCR identified LAB isolates as uncultured bacteria. Eight primers were used for RAPD and primer P69 highly discriminated the six isolates. The results from RAPD suggested that the strains of (Lactobacillus acidophilus were different from each other depending on the source of honey. 2018-04-23T02:33:25Z 2018-04-23T02:33:25Z 2012 Thesis http://ddms.usim.edu.my:80/jspui/handle/123456789/16017 en Full text Universiti Sains Islam Malaysia
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language English
topic Lactic acid bacteria
Honey
spellingShingle Lactic acid bacteria
Honey
Mohamed Mustafa Aween
Isolation, characterization and antibacterial activity of Lactic Acid Bacteria from commercial honey marketed in Malaysia
description Thesis (M.Sc)-- Faculty of Science and Technology, Universiti Sains Islam Malaysia, 2012
format Thesis
author Mohamed Mustafa Aween
author_facet Mohamed Mustafa Aween
author_sort Mohamed Mustafa Aween
title Isolation, characterization and antibacterial activity of Lactic Acid Bacteria from commercial honey marketed in Malaysia
title_short Isolation, characterization and antibacterial activity of Lactic Acid Bacteria from commercial honey marketed in Malaysia
title_full Isolation, characterization and antibacterial activity of Lactic Acid Bacteria from commercial honey marketed in Malaysia
title_fullStr Isolation, characterization and antibacterial activity of Lactic Acid Bacteria from commercial honey marketed in Malaysia
title_full_unstemmed Isolation, characterization and antibacterial activity of Lactic Acid Bacteria from commercial honey marketed in Malaysia
title_sort isolation, characterization and antibacterial activity of lactic acid bacteria from commercial honey marketed in malaysia
publisher Universiti Sains Islam Malaysia
publishDate 2018
url http://ddms.usim.edu.my:80/jspui/handle/123456789/16017
_version_ 1645154076772532224
score 13.222552

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