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The effect of gamma irradiation on the chemical structure and surface characteristics of dipalmitoylphosphatidylcholine (DPPC) [Kesan sinaran gama ke atas struktur kimia dan ciri permukaan dipalmitoilfosfatidilkolina (DPPC)]

Dipalmitoylphosphatidylcholine (DPPC) is one of the most abundant lipids in the human body and carries out important physiological functions such as liver protection and fighting infections. This study was conducted to investigate the effect of ionising radiation on the chemical structure and surfac...

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Main Authors: Mohd H.M.K., Ahmad A.F., Mohamed F., Kamaruzaman N.S., Ling N.W., Jin P.R.,, Yasir M.S., Isa M.H.M., Ramli S., Radiman S., Rahman I.A.
Format: Article
Language:English
Published: 2017
Subjects:
Dipalmitoylphosphatidylcholine (DPPC); Gamma radiation; High Performance Liquid-Chromatography (HPLC); Langmuir monolayer; Mass-Spectrometer (MS)
Online Access:http://ddms.usim.edu.my:80/jspui/handle/123456789/15493
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spelling my.usim-154932018-04-27T03:04:16Z The effect of gamma irradiation on the chemical structure and surface characteristics of dipalmitoylphosphatidylcholine (DPPC) [Kesan sinaran gama ke atas struktur kimia dan ciri permukaan dipalmitoilfosfatidilkolina (DPPC)] Mohd H.M.K., Ahmad A.F. Mohamed F., Kamaruzaman N.S. Ling N.W., Jin P.R., Yasir M.S., Isa M.H.M. Ramli S., Radiman S. Rahman I.A. Dipalmitoylphosphatidylcholine (DPPC); Gamma radiation; High Performance Liquid-Chromatography (HPLC); Langmuir monolayer; Mass-Spectrometer (MS) Dipalmitoylphosphatidylcholine (DPPC) is one of the most abundant lipids in the human body and carries out important physiological functions such as liver protection and fighting infections. This study was conducted to investigate the effect of ionising radiation on the chemical structure and surface characteristic of DPPC. Synthetic DPPC was dissolved in chloroform (1mg/ml) and irradiated with Cobalt-60 (dose range of 50 - 200 Gy). The change in surface characteristics due to gamma irradiation was determined by means of monolayer compression isotherms using a Langmuir trough. From the change in the isotherm features, the threshold dose that affected the surface characteristics of the DPPC monolayer was determined to be 60 Gy. With increasing dose value, the plateau feature in the DPPC isotherm became shorter and was shifted to higher surface pressures. Analysis using High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) suggested that gamma irradiation of DPPC destroyed the chemical structure of DPPC and produced two main radiolytic products, namely lisophosphatidylcholine, LPC (~495.3142 g/mol) and phosphatidic acid, PA (~718.916 g/mol) with an average percentage of LPC and PA of 23% and 74%, respectively. 2017-12-04T09:06:40Z 2017-12-04T09:06:40Z 2013 Article 13942506 http://ddms.usim.edu.my:80/jspui/handle/123456789/15493 en
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language English
topic Dipalmitoylphosphatidylcholine (DPPC); Gamma radiation; High Performance Liquid-Chromatography (HPLC); Langmuir monolayer; Mass-Spectrometer (MS)
spellingShingle Dipalmitoylphosphatidylcholine (DPPC); Gamma radiation; High Performance Liquid-Chromatography (HPLC); Langmuir monolayer; Mass-Spectrometer (MS)
Mohd H.M.K., Ahmad A.F.
Mohamed F., Kamaruzaman N.S.
Ling N.W., Jin P.R.,
Yasir M.S., Isa M.H.M.
Ramli S., Radiman S.
Rahman I.A.
The effect of gamma irradiation on the chemical structure and surface characteristics of dipalmitoylphosphatidylcholine (DPPC) [Kesan sinaran gama ke atas struktur kimia dan ciri permukaan dipalmitoilfosfatidilkolina (DPPC)]
description Dipalmitoylphosphatidylcholine (DPPC) is one of the most abundant lipids in the human body and carries out important physiological functions such as liver protection and fighting infections. This study was conducted to investigate the effect of ionising radiation on the chemical structure and surface characteristic of DPPC. Synthetic DPPC was dissolved in chloroform (1mg/ml) and irradiated with Cobalt-60 (dose range of 50 - 200 Gy). The change in surface characteristics due to gamma irradiation was determined by means of monolayer compression isotherms using a Langmuir trough. From the change in the isotherm features, the threshold dose that affected the surface characteristics of the DPPC monolayer was determined to be 60 Gy. With increasing dose value, the plateau feature in the DPPC isotherm became shorter and was shifted to higher surface pressures. Analysis using High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) suggested that gamma irradiation of DPPC destroyed the chemical structure of DPPC and produced two main radiolytic products, namely lisophosphatidylcholine, LPC (~495.3142 g/mol) and phosphatidic acid, PA (~718.916 g/mol) with an average percentage of LPC and PA of 23% and 74%, respectively.
format Article
author Mohd H.M.K., Ahmad A.F.
Mohamed F., Kamaruzaman N.S.
Ling N.W., Jin P.R.,
Yasir M.S., Isa M.H.M.
Ramli S., Radiman S.
Rahman I.A.
author_facet Mohd H.M.K., Ahmad A.F.
Mohamed F., Kamaruzaman N.S.
Ling N.W., Jin P.R.,
Yasir M.S., Isa M.H.M.
Ramli S., Radiman S.
Rahman I.A.
author_sort Mohd H.M.K., Ahmad A.F.
title The effect of gamma irradiation on the chemical structure and surface characteristics of dipalmitoylphosphatidylcholine (DPPC) [Kesan sinaran gama ke atas struktur kimia dan ciri permukaan dipalmitoilfosfatidilkolina (DPPC)]
title_short The effect of gamma irradiation on the chemical structure and surface characteristics of dipalmitoylphosphatidylcholine (DPPC) [Kesan sinaran gama ke atas struktur kimia dan ciri permukaan dipalmitoilfosfatidilkolina (DPPC)]
title_full The effect of gamma irradiation on the chemical structure and surface characteristics of dipalmitoylphosphatidylcholine (DPPC) [Kesan sinaran gama ke atas struktur kimia dan ciri permukaan dipalmitoilfosfatidilkolina (DPPC)]
title_fullStr The effect of gamma irradiation on the chemical structure and surface characteristics of dipalmitoylphosphatidylcholine (DPPC) [Kesan sinaran gama ke atas struktur kimia dan ciri permukaan dipalmitoilfosfatidilkolina (DPPC)]
title_full_unstemmed The effect of gamma irradiation on the chemical structure and surface characteristics of dipalmitoylphosphatidylcholine (DPPC) [Kesan sinaran gama ke atas struktur kimia dan ciri permukaan dipalmitoilfosfatidilkolina (DPPC)]
title_sort effect of gamma irradiation on the chemical structure and surface characteristics of dipalmitoylphosphatidylcholine (dppc) [kesan sinaran gama ke atas struktur kimia dan ciri permukaan dipalmitoilfosfatidilkolina (dppc)]
publishDate 2017
url http://ddms.usim.edu.my:80/jspui/handle/123456789/15493
_version_ 1645153931713576960
score 13.222552

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