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Sensitivity of acanthamoeba cyst to antimicrobial agents

Introduction: Acanthamoeba is an ubiquitous free-living protozoa which causes serious ocular problems. Acanthamoeba keratitis is becoming more prevalent amongst contact lens wearers. The disease can cause loss of vision and blindness if not treated properly. The objective of this research is to stud...

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Main Authors:	Noradilah S.A., Mohamed Kamel A.G., Anisah N., Noraina A.R., Yusof S.
Format:	Article
Language:	English
Published:	2017
Subjects:	Acanthamoeba; Chlorhexidine; Polyhexamethylene biguanide; Sensitivity
Online Access:	http://ddms.usim.edu.my:80/jspui/handle/123456789/15341
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spelling	my.usim-153412017-10-30T05:03:07Z Sensitivity of acanthamoeba cyst to antimicrobial agents Noradilah S.A., Mohamed Kamel A.G. Anisah N., Noraina A.R. Yusof S. Acanthamoeba; Chlorhexidine; Polyhexamethylene biguanide; Sensitivity Introduction: Acanthamoeba is an ubiquitous free-living protozoa which causes serious ocular problems. Acanthamoeba keratitis is becoming more prevalent amongst contact lens wearers. The disease can cause loss of vision and blindness if not treated properly. The objective of this research is to study the sensitivity of six Acanthamoeba spp. isolates, of which three were from the clinical isolates (HKL 95, HTH 40 and HS 6) and the remaining three from environmental isolates (TTT 9, TL 3 and SMAL 8) to antimicrobial agents. Methods: The antimicrobial agents chosen for this purpose were polyhexamethylene biguanide (PHMB) and Chlorhexidine. Serial dilutions were perfomed forpolyhexamemylene biguanide and Chlorhexidine. Cyst suspensions from the chosen isolates were exposed to PHMB and Chlorhexidine respectively. After 48 hours incubation time at 30°C, each mixture was filtered and filtration membrane was put onto non-nutrient agar laid with Escherichia coli. The agar plates were incubated for three days at 30°C and examined daily until day 14 to detect the presence of Acanthamoeba trophozoites under the inverted microscope. The presence of trophozoites indicated me ineffectiveness of the antimicrobial agents. Results: Both of the antimicrobial agents tested were found to be effective against Acanthamoeba cysts from all die test strains. Polyhexamethylene biguanide gave a minimum cysticidal concentration (MCC) mean value of 2.848 ug/mL while Chlorhexidine showed MCC mean value at a concentration of 3.988 μg/mL. Contusion: It can be concluded that me Acanthamoeba cysts were sensitive to polyhexamethylene biguanide and Chlorhexidine. 2017-10-30T05:03:07Z 2017-10-30T05:03:07Z 2010 Article 16758544 http://ddms.usim.edu.my:80/jspui/handle/123456789/15341 en
institution	Universiti Sains Islam Malaysia
building	USIM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universit Sains Islam i Malaysia
content_source	USIM Institutional Repository
url_provider	http://ddms.usim.edu.my/
language	English
topic	Acanthamoeba; Chlorhexidine; Polyhexamethylene biguanide; Sensitivity
spellingShingle	Acanthamoeba; Chlorhexidine; Polyhexamethylene biguanide; Sensitivity Noradilah S.A., Mohamed Kamel A.G. Anisah N., Noraina A.R. Yusof S. Sensitivity of acanthamoeba cyst to antimicrobial agents
description	Introduction: Acanthamoeba is an ubiquitous free-living protozoa which causes serious ocular problems. Acanthamoeba keratitis is becoming more prevalent amongst contact lens wearers. The disease can cause loss of vision and blindness if not treated properly. The objective of this research is to study the sensitivity of six Acanthamoeba spp. isolates, of which three were from the clinical isolates (HKL 95, HTH 40 and HS 6) and the remaining three from environmental isolates (TTT 9, TL 3 and SMAL 8) to antimicrobial agents. Methods: The antimicrobial agents chosen for this purpose were polyhexamethylene biguanide (PHMB) and Chlorhexidine. Serial dilutions were perfomed forpolyhexamemylene biguanide and Chlorhexidine. Cyst suspensions from the chosen isolates were exposed to PHMB and Chlorhexidine respectively. After 48 hours incubation time at 30°C, each mixture was filtered and filtration membrane was put onto non-nutrient agar laid with Escherichia coli. The agar plates were incubated for three days at 30°C and examined daily until day 14 to detect the presence of Acanthamoeba trophozoites under the inverted microscope. The presence of trophozoites indicated me ineffectiveness of the antimicrobial agents. Results: Both of the antimicrobial agents tested were found to be effective against Acanthamoeba cysts from all die test strains. Polyhexamethylene biguanide gave a minimum cysticidal concentration (MCC) mean value of 2.848 ug/mL while Chlorhexidine showed MCC mean value at a concentration of 3.988 μg/mL. Contusion: It can be concluded that me Acanthamoeba cysts were sensitive to polyhexamethylene biguanide and Chlorhexidine.
format	Article
author	Noradilah S.A., Mohamed Kamel A.G. Anisah N., Noraina A.R. Yusof S.
author_facet	Noradilah S.A., Mohamed Kamel A.G. Anisah N., Noraina A.R. Yusof S.
author_sort	Noradilah S.A., Mohamed Kamel A.G.
title	Sensitivity of acanthamoeba cyst to antimicrobial agents
title_short	Sensitivity of acanthamoeba cyst to antimicrobial agents
title_full	Sensitivity of acanthamoeba cyst to antimicrobial agents
title_fullStr	Sensitivity of acanthamoeba cyst to antimicrobial agents
title_full_unstemmed	Sensitivity of acanthamoeba cyst to antimicrobial agents
title_sort	sensitivity of acanthamoeba cyst to antimicrobial agents
publishDate	2017
url	http://ddms.usim.edu.my:80/jspui/handle/123456789/15341
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score	13.222552

Sensitivity of acanthamoeba cyst to antimicrobial agents

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