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A comparison between using Peripheral Whole Blood and Buccal Swab For Red Cell Genotyping amongst Multiply-Transfused Thalassaemia patients

Blood has always been the main sample in determining the different blood group types. However, in multiply-transfused patients, accurate red cell antigen typing by serology is a constant problem due to the exposure to donor‟s blood in patient‟s circulation making results unreliable. To overcome thes...

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Main Author: Nadila Haryani Osman
Format: Thesis
Language:en_US
Published: Universiti Sains Islam malaysia 2016
Subjects:
Thalassemia
Online Access:http://ddms.usim.edu.my:80/jspui/handle/123456789/12502
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spelling my.usim-125022019-04-11T04:22:28Z A comparison between using Peripheral Whole Blood and Buccal Swab For Red Cell Genotyping amongst Multiply-Transfused Thalassaemia patients Nadila Haryani Osman Thalassemia Blood has always been the main sample in determining the different blood group types. However, in multiply-transfused patients, accurate red cell antigen typing by serology is a constant problem due to the exposure to donor‟s blood in patient‟s circulation making results unreliable. To overcome these problems, another source and technique for blood typing is needed. The main aim of this study was to investigate the genotype of red cells for RH (C, c, E, e), KEL (Kell, Celano), Kidd (JKA, JKB) and Duffy (FYA, FYB) using buccal swab and peripheral whole blood amongst multiply-transfused thalassaemia patients. Sixty-three of multiply-transfused thalassaemia patients from the Thalassaemia Clinic of Ampang Hospital and Universiti Kebangsaan Malaysia Medical Centre participated in this study. Paired samples consisting of peripheral whole blood and buccal swab samples were collected prior to the scheduled blood transfusion and on day 7 after the transfusion. Blood samples were subjected to serological phenotyping by tube method and DNA genotyping while buccal swab was subjected to DNA genotyping only. Blood genotyping was performed using TaqMan® Single Nucleotide Polymorphism Real Time Polymerase Chain Reaction (SNP RT-PCR) assays for RHEe, RHCc, Kidd, KEL and Duffy blood group systems. Complete data was available in 33 patients only. Discrepancies were found between the phenotype and genotype results for all blood groups tested in both pre- and post-transfusion samples. However, a full concordance of genotyping result between pre- and post-transfusion samples was observed. When comparing the genotyping results between different sampling methods, blood and buccal swab samples showed concordant results. Accurate red blood cell antigen profiling is important for patients requiring multiple transfusions. The SNP RT-PCR platform is a reliable alternative to the conventional method. Buccal samples offer a simple and inexpensive alternative collection method that may be used for accurate blood group genotyping when blood samples are unavailable. 2016-10-25T07:21:35Z 2016-10-25T07:21:35Z 2016-05 Thesis http://ddms.usim.edu.my:80/jspui/handle/123456789/12502 en_US Universiti Sains Islam malaysia
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language en_US
topic Thalassemia
spellingShingle Thalassemia
Nadila Haryani Osman
A comparison between using Peripheral Whole Blood and Buccal Swab For Red Cell Genotyping amongst Multiply-Transfused Thalassaemia patients
description Blood has always been the main sample in determining the different blood group types. However, in multiply-transfused patients, accurate red cell antigen typing by serology is a constant problem due to the exposure to donor‟s blood in patient‟s circulation making results unreliable. To overcome these problems, another source and technique for blood typing is needed. The main aim of this study was to investigate the genotype of red cells for RH (C, c, E, e), KEL (Kell, Celano), Kidd (JKA, JKB) and Duffy (FYA, FYB) using buccal swab and peripheral whole blood amongst multiply-transfused thalassaemia patients. Sixty-three of multiply-transfused thalassaemia patients from the Thalassaemia Clinic of Ampang Hospital and Universiti Kebangsaan Malaysia Medical Centre participated in this study. Paired samples consisting of peripheral whole blood and buccal swab samples were collected prior to the scheduled blood transfusion and on day 7 after the transfusion. Blood samples were subjected to serological phenotyping by tube method and DNA genotyping while buccal swab was subjected to DNA genotyping only. Blood genotyping was performed using TaqMan® Single Nucleotide Polymorphism Real Time Polymerase Chain Reaction (SNP RT-PCR) assays for RHEe, RHCc, Kidd, KEL and Duffy blood group systems. Complete data was available in 33 patients only. Discrepancies were found between the phenotype and genotype results for all blood groups tested in both pre- and post-transfusion samples. However, a full concordance of genotyping result between pre- and post-transfusion samples was observed. When comparing the genotyping results between different sampling methods, blood and buccal swab samples showed concordant results. Accurate red blood cell antigen profiling is important for patients requiring multiple transfusions. The SNP RT-PCR platform is a reliable alternative to the conventional method. Buccal samples offer a simple and inexpensive alternative collection method that may be used for accurate blood group genotyping when blood samples are unavailable.
format Thesis
author Nadila Haryani Osman
author_facet Nadila Haryani Osman
author_sort Nadila Haryani Osman
title A comparison between using Peripheral Whole Blood and Buccal Swab For Red Cell Genotyping amongst Multiply-Transfused Thalassaemia patients
title_short A comparison between using Peripheral Whole Blood and Buccal Swab For Red Cell Genotyping amongst Multiply-Transfused Thalassaemia patients
title_full A comparison between using Peripheral Whole Blood and Buccal Swab For Red Cell Genotyping amongst Multiply-Transfused Thalassaemia patients
title_fullStr A comparison between using Peripheral Whole Blood and Buccal Swab For Red Cell Genotyping amongst Multiply-Transfused Thalassaemia patients
title_full_unstemmed A comparison between using Peripheral Whole Blood and Buccal Swab For Red Cell Genotyping amongst Multiply-Transfused Thalassaemia patients
title_sort comparison between using peripheral whole blood and buccal swab for red cell genotyping amongst multiply-transfused thalassaemia patients
publisher Universiti Sains Islam malaysia
publishDate 2016
url http://ddms.usim.edu.my:80/jspui/handle/123456789/12502
_version_ 1645153185514389504
score 13.222552

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