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The effect of storage at -20°C and -80°C on HbA1c measurements in whole blood samples analysed using Bio-Rad D-10

Introduction: Glycated haemoglobin (HbA1c) is a test commonly measured in many clinical laboratories to monitor and diagnose diabetes mellitus (DM). The laboratory in Hospital Tengku Ampuan Afzan (HTAA) receives HbA1c samples internally and from several other hospitals and clinics in the state of Pa...

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Main Authors: T. Ibrahim, T. Zaharah, Samsudin, Intan Nureslyna, Nasuruddin, Alia Nasriana, Thambiah, Subashini C.
Format: Article
Language:English
Published: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2022
Online Access:http://psasir.upm.edu.my/id/eprint/99300/1/2022121911551208_MJMHS_0449.pdf
http://psasir.upm.edu.my/id/eprint/99300/
https://medic.upm.edu.my/upload/dokumen/2022121911551208_MJMHS_0449.pdf
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spelling my.upm.eprints.993002023-03-06T07:44:53Z http://psasir.upm.edu.my/id/eprint/99300/ The effect of storage at -20°C and -80°C on HbA1c measurements in whole blood samples analysed using Bio-Rad D-10 T. Ibrahim, T. Zaharah Samsudin, Intan Nureslyna Nasuruddin, Alia Nasriana Thambiah, Subashini C. Introduction: Glycated haemoglobin (HbA1c) is a test commonly measured in many clinical laboratories to monitor and diagnose diabetes mellitus (DM). The laboratory in Hospital Tengku Ampuan Afzan (HTAA) receives HbA1c samples internally and from several other hospitals and clinics in the state of Pahang, at which, before transportation and analysis, the samples are often stored. This study determined the stability of HbA1c samples following different storage temperatures and duration for up to 30 days. Method: Whole blood samples for HbA1c analysis were col- lected from 222 healthy blood donors and type 2 DM (T2DM) patients. Each sample was prepared into four aliquots, which were then stored at temperatures -20°C and -80°C. HbA1c analyses were performed at baseline, days 15 and 30 using ion-exchange high-performance liquid chromatography (HPLC) assay technique on Bio-Rad D-10 analy- ser. HbA1c levels following sample storage were compared to the levels at baseline. Results: The baseline HbA1c (mean±SD) was 6.6±2.2%. At -20°C of storage, the HbA1c levels decreased with a mean difference of 0.1% and 0.3% on days 15 and 30, respectively. Storage at -80°C resulted in a mean difference of 0.1% on day 15 whilst no change was noted on day 30. The number of samples that showed clinically significant HbA1c change (defined as a change of >0.5%) was less for the samples stored at -80°C (4.5%) as compared to -20°C (23%). Conclusion: HbA1c concentration is affected by temperature and duration of sample storage. In instances where prolonged storage of HbA1c samples is expected, storage at -80°C is preferable. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2022-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/99300/1/2022121911551208_MJMHS_0449.pdf T. Ibrahim, T. Zaharah and Samsudin, Intan Nureslyna and Nasuruddin, Alia Nasriana and Thambiah, Subashini C. (2022) The effect of storage at -20°C and -80°C on HbA1c measurements in whole blood samples analysed using Bio-Rad D-10. Malaysian Journal of Medicine and Health Sciences, 18 (supp.21). pp. 54-58. ISSN 2636-9346 https://medic.upm.edu.my/upload/dokumen/2022121911551208_MJMHS_0449.pdf 10.47836/mjmhs18.s21.9
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Introduction: Glycated haemoglobin (HbA1c) is a test commonly measured in many clinical laboratories to monitor and diagnose diabetes mellitus (DM). The laboratory in Hospital Tengku Ampuan Afzan (HTAA) receives HbA1c samples internally and from several other hospitals and clinics in the state of Pahang, at which, before transportation and analysis, the samples are often stored. This study determined the stability of HbA1c samples following different storage temperatures and duration for up to 30 days. Method: Whole blood samples for HbA1c analysis were col- lected from 222 healthy blood donors and type 2 DM (T2DM) patients. Each sample was prepared into four aliquots, which were then stored at temperatures -20°C and -80°C. HbA1c analyses were performed at baseline, days 15 and 30 using ion-exchange high-performance liquid chromatography (HPLC) assay technique on Bio-Rad D-10 analy- ser. HbA1c levels following sample storage were compared to the levels at baseline. Results: The baseline HbA1c (mean±SD) was 6.6±2.2%. At -20°C of storage, the HbA1c levels decreased with a mean difference of 0.1% and 0.3% on days 15 and 30, respectively. Storage at -80°C resulted in a mean difference of 0.1% on day 15 whilst no change was noted on day 30. The number of samples that showed clinically significant HbA1c change (defined as a change of >0.5%) was less for the samples stored at -80°C (4.5%) as compared to -20°C (23%). Conclusion: HbA1c concentration is affected by temperature and duration of sample storage. In instances where prolonged storage of HbA1c samples is expected, storage at -80°C is preferable.
format Article
author T. Ibrahim, T. Zaharah
Samsudin, Intan Nureslyna
Nasuruddin, Alia Nasriana
Thambiah, Subashini C.
spellingShingle T. Ibrahim, T. Zaharah
Samsudin, Intan Nureslyna
Nasuruddin, Alia Nasriana
Thambiah, Subashini C.
The effect of storage at -20°C and -80°C on HbA1c measurements in whole blood samples analysed using Bio-Rad D-10
author_facet T. Ibrahim, T. Zaharah
Samsudin, Intan Nureslyna
Nasuruddin, Alia Nasriana
Thambiah, Subashini C.
author_sort T. Ibrahim, T. Zaharah
title The effect of storage at -20°C and -80°C on HbA1c measurements in whole blood samples analysed using Bio-Rad D-10
title_short The effect of storage at -20°C and -80°C on HbA1c measurements in whole blood samples analysed using Bio-Rad D-10
title_full The effect of storage at -20°C and -80°C on HbA1c measurements in whole blood samples analysed using Bio-Rad D-10
title_fullStr The effect of storage at -20°C and -80°C on HbA1c measurements in whole blood samples analysed using Bio-Rad D-10
title_full_unstemmed The effect of storage at -20°C and -80°C on HbA1c measurements in whole blood samples analysed using Bio-Rad D-10
title_sort effect of storage at -20°c and -80°c on hba1c measurements in whole blood samples analysed using bio-rad d-10
publisher Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
publishDate 2022
url http://psasir.upm.edu.my/id/eprint/99300/1/2022121911551208_MJMHS_0449.pdf
http://psasir.upm.edu.my/id/eprint/99300/
https://medic.upm.edu.my/upload/dokumen/2022121911551208_MJMHS_0449.pdf
_version_ 1759690995893534720
score 13.211869

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