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Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia

The emergence of the Fusarium wilt of banana (Panama disease) caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (Fusarium odoratissimum) which caused loss to the global banana production is known to infect majority banana cultivar. A total of 38 isolates of Fusarium spp. related to b...

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Main Author: Ujat, Anysia Hedy
Format: Thesis
Language:English
Published: 2021
Subjects:
Fusarium wilt of banana
Bananas - Diseases and pests
Online Access:http://psasir.upm.edu.my/id/eprint/98594/1/FP%202021%2048%20UPMIR.pdf
http://psasir.upm.edu.my/id/eprint/98594/
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spelling my.upm.eprints.985942022-09-06T07:03:20Z http://psasir.upm.edu.my/id/eprint/98594/ Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia Ujat, Anysia Hedy The emergence of the Fusarium wilt of banana (Panama disease) caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (Fusarium odoratissimum) which caused loss to the global banana production is known to infect majority banana cultivar. A total of 38 isolates of Fusarium spp. related to banana Fusarium wilt obtained from the Biological Control Laboratory, Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia were cultured on different media for observation of culture characteristics. Based on the culture morphology, isolates are grouped into four different group and a representative isolate were chosen for each group, namely Prk 4-1, Ns 5-2, Phg 3 and Swk 4. Micromorphology shows no significant difference in the macro- and microconidia size. To further identify the isolates, molecular analysis was conducted by using the sequences of Histone H3 gene and TEF-1α gene. TR4 specific primers were used to confirm the presence of Fusarium odoratissimum (TR4). The phylogenetic tree using Histone H3 sequences showed all isolates grouped into Fusarium oxysporum species complex while phylogenetic tree using TEF-1α sequences revealed that the samples were composed of 27 isolates of Fusarium odoratissimum (TR4), two isolate Fusarium grosmichelii (R1), six unknown Fusarium oxysporum species complexes isolates and three Fusarium fujikuroi isolates. By using TR4 specific primers, all 27 samples were confirmed to be Fusarium odoratissimum (TR4). To study the susceptibility of local banana cultivar, pathogenicity test was conducted on 3 months old banana plantlets consisting of five different local banana cultivars of three different genome types: Musa acuminata AAA (Pisang Berangan and Pisang Serendah), Musa paradisiaca AAB (Pisang Nangka and Pisang Raja) and Musa balbisiana BBB (Pisang Nipah). Observation was recorded for 30 days, and foliar symptoms showed different severity of disease progression. However, internal symptoms showed that all of the inoculated plantlets were severely infected. Cooking banana (AAB and BBB) of local cultivar were also found to be susceptible to Fusarium odoratissimum (TR4). This study shows that there are not only Fusarium odoratissimum (TR4) presence in Malaysia but also Fusarium grosmichelii (R1) and a possibility of an unknown strain of Fusarium oxysporum. Pathogenicity test reveals that local banana cultivar in Malaysia are susceptible to Fusarium wilt disease regardless of the genotype of the banana plants. These findings will aid in the substantive documentation of Fusarium spp. in the local banana plantation scenario. 2021-02 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/98594/1/FP%202021%2048%20UPMIR.pdf Ujat, Anysia Hedy (2021) Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia. Masters thesis, Universiti Putra Malaysia. Fusarium wilt of banana Bananas - Diseases and pests
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
topic Fusarium wilt of banana
Bananas - Diseases and pests
spellingShingle Fusarium wilt of banana
Bananas - Diseases and pests
Ujat, Anysia Hedy
Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia
description The emergence of the Fusarium wilt of banana (Panama disease) caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (Fusarium odoratissimum) which caused loss to the global banana production is known to infect majority banana cultivar. A total of 38 isolates of Fusarium spp. related to banana Fusarium wilt obtained from the Biological Control Laboratory, Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia were cultured on different media for observation of culture characteristics. Based on the culture morphology, isolates are grouped into four different group and a representative isolate were chosen for each group, namely Prk 4-1, Ns 5-2, Phg 3 and Swk 4. Micromorphology shows no significant difference in the macro- and microconidia size. To further identify the isolates, molecular analysis was conducted by using the sequences of Histone H3 gene and TEF-1α gene. TR4 specific primers were used to confirm the presence of Fusarium odoratissimum (TR4). The phylogenetic tree using Histone H3 sequences showed all isolates grouped into Fusarium oxysporum species complex while phylogenetic tree using TEF-1α sequences revealed that the samples were composed of 27 isolates of Fusarium odoratissimum (TR4), two isolate Fusarium grosmichelii (R1), six unknown Fusarium oxysporum species complexes isolates and three Fusarium fujikuroi isolates. By using TR4 specific primers, all 27 samples were confirmed to be Fusarium odoratissimum (TR4). To study the susceptibility of local banana cultivar, pathogenicity test was conducted on 3 months old banana plantlets consisting of five different local banana cultivars of three different genome types: Musa acuminata AAA (Pisang Berangan and Pisang Serendah), Musa paradisiaca AAB (Pisang Nangka and Pisang Raja) and Musa balbisiana BBB (Pisang Nipah). Observation was recorded for 30 days, and foliar symptoms showed different severity of disease progression. However, internal symptoms showed that all of the inoculated plantlets were severely infected. Cooking banana (AAB and BBB) of local cultivar were also found to be susceptible to Fusarium odoratissimum (TR4). This study shows that there are not only Fusarium odoratissimum (TR4) presence in Malaysia but also Fusarium grosmichelii (R1) and a possibility of an unknown strain of Fusarium oxysporum. Pathogenicity test reveals that local banana cultivar in Malaysia are susceptible to Fusarium wilt disease regardless of the genotype of the banana plants. These findings will aid in the substantive documentation of Fusarium spp. in the local banana plantation scenario.
format Thesis
author Ujat, Anysia Hedy
author_facet Ujat, Anysia Hedy
author_sort Ujat, Anysia Hedy
title Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia
title_short Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia
title_full Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia
title_fullStr Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia
title_full_unstemmed Characterization and pathogenicity of Fusarium species complex associated with Fusarium wilt of banana in Malaysia
title_sort characterization and pathogenicity of fusarium species complex associated with fusarium wilt of banana in malaysia
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/98594/1/FP%202021%2048%20UPMIR.pdf
http://psasir.upm.edu.my/id/eprint/98594/
_version_ 1744355334058672128
score 13.211869

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