Cover Image

Detection of pathogenic Leptospira in rats and phylogenetic analysis using outer membrane lipoprotein Lipl32 gene at two major public markets

Introduction: Limited studies have been documented on the presence of pathogenic Leptospira in public markets serving the community in sub-districts of Selangor. The aim of this study was to detect the presence of pathogenic Leptospira in rats using a gene encoding an outer membrane lipoprotein LipL...

Full description

Saved in:
Bibliographic Details
Main Authors: Wan, Steffi Julan, Sulaiman, Nurshahira, Zulkifli, Nurul Farhana, Neela, Vasantha Kumari, Hui, Yee Chee, Masri, Siti Norbaya, Mohamad Ikbal, Nurul Huda, Bhassu, Subha, Omar, Hasmahzaiti, M. Jamil Al-Obaidi, Mazen, Mohd Desa, Mohd Nasir
Format: Article
Language:English
Published: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2022
Online Access:http://psasir.upm.edu.my/id/eprint/98126/1/2022031418312901_MJMHS_0093.pdf
http://psasir.upm.edu.my/id/eprint/98126/
https://medic.upm.edu.my/upload/dokumen/2022031418312901_MJMHS_0093.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.98126
record_format eprints
spelling my.upm.eprints.981262022-08-11T08:28:23Z http://psasir.upm.edu.my/id/eprint/98126/ Detection of pathogenic Leptospira in rats and phylogenetic analysis using outer membrane lipoprotein Lipl32 gene at two major public markets Wan, Steffi Julan Sulaiman, Nurshahira Zulkifli, Nurul Farhana Neela, Vasantha Kumari Hui, Yee Chee Masri, Siti Norbaya Mohamad Ikbal, Nurul Huda Bhassu, Subha Omar, Hasmahzaiti M. Jamil Al-Obaidi, Mazen Mohd Desa, Mohd Nasir Introduction: Limited studies have been documented on the presence of pathogenic Leptospira in public markets serving the community in sub-districts of Selangor. The aim of this study was to detect the presence of pathogenic Leptospira in rats using a gene encoding an outer membrane lipoprotein LipL32. Methods: Polymerase chain reaction (PCR) was performed using LipL32 primers on sixty kidney samples of rats trapped at two locations of study; Pasar Borong Selangor in Seri Kembangan and Pasar Basah Bandar Baru Bangi in Bangi. Results: Out of 60 samples analysed, 36.7% were positive for the presence of LipL32. All positive samples highly matched (>94%) nucleotide sequence for LipL32 of pathogenic Leptospira and related to the pathogens through phylogenetic analysis. Conclusion: The detection of LipL32 indicates the potential presence of pathogenic Leptospira species at public markets. Although only 60 rats were successfully trapped, the rats are mobile and might further transmit the pathogenic organisms to other areas. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2022-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/98126/1/2022031418312901_MJMHS_0093.pdf Wan, Steffi Julan and Sulaiman, Nurshahira and Zulkifli, Nurul Farhana and Neela, Vasantha Kumari and Hui, Yee Chee and Masri, Siti Norbaya and Mohamad Ikbal, Nurul Huda and Bhassu, Subha and Omar, Hasmahzaiti and M. Jamil Al-Obaidi, Mazen and Mohd Desa, Mohd Nasir (2022) Detection of pathogenic Leptospira in rats and phylogenetic analysis using outer membrane lipoprotein Lipl32 gene at two major public markets. Malaysian Journal of Medicine and Health Sciences, 18 (2). pp. 1-5. ISSN 1675-8544; ESSN: 2636-9346 https://medic.upm.edu.my/upload/dokumen/2022031418312901_MJMHS_0093.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Introduction: Limited studies have been documented on the presence of pathogenic Leptospira in public markets serving the community in sub-districts of Selangor. The aim of this study was to detect the presence of pathogenic Leptospira in rats using a gene encoding an outer membrane lipoprotein LipL32. Methods: Polymerase chain reaction (PCR) was performed using LipL32 primers on sixty kidney samples of rats trapped at two locations of study; Pasar Borong Selangor in Seri Kembangan and Pasar Basah Bandar Baru Bangi in Bangi. Results: Out of 60 samples analysed, 36.7% were positive for the presence of LipL32. All positive samples highly matched (>94%) nucleotide sequence for LipL32 of pathogenic Leptospira and related to the pathogens through phylogenetic analysis. Conclusion: The detection of LipL32 indicates the potential presence of pathogenic Leptospira species at public markets. Although only 60 rats were successfully trapped, the rats are mobile and might further transmit the pathogenic organisms to other areas.
format Article
author Wan, Steffi Julan
Sulaiman, Nurshahira
Zulkifli, Nurul Farhana
Neela, Vasantha Kumari
Hui, Yee Chee
Masri, Siti Norbaya
Mohamad Ikbal, Nurul Huda
Bhassu, Subha
Omar, Hasmahzaiti
M. Jamil Al-Obaidi, Mazen
Mohd Desa, Mohd Nasir
spellingShingle Wan, Steffi Julan
Sulaiman, Nurshahira
Zulkifli, Nurul Farhana
Neela, Vasantha Kumari
Hui, Yee Chee
Masri, Siti Norbaya
Mohamad Ikbal, Nurul Huda
Bhassu, Subha
Omar, Hasmahzaiti
M. Jamil Al-Obaidi, Mazen
Mohd Desa, Mohd Nasir
Detection of pathogenic Leptospira in rats and phylogenetic analysis using outer membrane lipoprotein Lipl32 gene at two major public markets
author_facet Wan, Steffi Julan
Sulaiman, Nurshahira
Zulkifli, Nurul Farhana
Neela, Vasantha Kumari
Hui, Yee Chee
Masri, Siti Norbaya
Mohamad Ikbal, Nurul Huda
Bhassu, Subha
Omar, Hasmahzaiti
M. Jamil Al-Obaidi, Mazen
Mohd Desa, Mohd Nasir
author_sort Wan, Steffi Julan
title Detection of pathogenic Leptospira in rats and phylogenetic analysis using outer membrane lipoprotein Lipl32 gene at two major public markets
title_short Detection of pathogenic Leptospira in rats and phylogenetic analysis using outer membrane lipoprotein Lipl32 gene at two major public markets
title_full Detection of pathogenic Leptospira in rats and phylogenetic analysis using outer membrane lipoprotein Lipl32 gene at two major public markets
title_fullStr Detection of pathogenic Leptospira in rats and phylogenetic analysis using outer membrane lipoprotein Lipl32 gene at two major public markets
title_full_unstemmed Detection of pathogenic Leptospira in rats and phylogenetic analysis using outer membrane lipoprotein Lipl32 gene at two major public markets
title_sort detection of pathogenic leptospira in rats and phylogenetic analysis using outer membrane lipoprotein lipl32 gene at two major public markets
publisher Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
publishDate 2022
url http://psasir.upm.edu.my/id/eprint/98126/1/2022031418312901_MJMHS_0093.pdf
http://psasir.upm.edu.my/id/eprint/98126/
https://medic.upm.edu.my/upload/dokumen/2022031418312901_MJMHS_0093.pdf
_version_ 1743108555748147200
score 13.211869

Similar Items