Staff View: Identification of Leptospira in water by Fe-Pd-doped polyaniline nanocomposite thin film

Identification of Leptospira in water by Fe-Pd-doped polyaniline nanocomposite thin film

Leptospirosis disease was caused by rat urine which contains the genus Leptospira bacteria. In this study, the fabrication of Pd-Fe-doped polyaniline nanocomposite thin films for the determination of the genus Leptospira bacteria thin films has been investigated. Pd-Fe-doped polyaniline nanocomposit...

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Main Authors:	Abdullah, Huda, Mustaza, Seri Mastura, Bejo, Siti Khairani, Yahya, Iskandar, Kamal, Noorfazila, Othman, Mohd Hafiz Dzarfan
Format:	Article
Language:	English
Published:	SAGE Publications 2021
Online Access:	http://psasir.upm.edu.my/id/eprint/97600/1/ABSTRACT.pdf http://psasir.upm.edu.my/id/eprint/97600/ https://journals.sagepub.com/doi/full/10.1177/18479804211011389
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spelling	my.upm.eprints.976002022-07-25T03:05:36Z http://psasir.upm.edu.my/id/eprint/97600/ Identification of Leptospira in water by Fe-Pd-doped polyaniline nanocomposite thin film Abdullah, Huda Mustaza, Seri Mastura Bejo, Siti Khairani Yahya, Iskandar Kamal, Noorfazila Othman, Mohd Hafiz Dzarfan Leptospirosis disease was caused by rat urine which contains the genus Leptospira bacteria. In this study, the fabrication of Pd-Fe-doped polyaniline nanocomposite thin films for the determination of the genus Leptospira bacteria thin films has been investigated. Pd-Fe-doped polyaniline nanocomposite thin films were fabricated by sol–gel spin coating method. The electrode sensors were immersed in the Leptospira solution. The resulting materials were investigated using field-emission scanning electron microscopy, atomic force microscopy, transmission electron microscopy, and current–voltage measurement. The atomic force microscopy images show the specific morphology films’ structure for Leptospira detection, whereas the field-emission scanning electron microscopy image shows the irregularity of clump nanoparticles in thin film surfaces. Transmission electron microscopy result shows that metal alloy (Fe-Pd) embedded in the polymer matrix. Current–voltage measurement with and without incubation of the thin film into Leptospira solution was done to show the relationship between concentration bacteria versus current. The result shows that polyaniline-Fe0.4-Pd0.6 nanocomposite thin film has higher sensitivity in detecting Leptospira, where it has performed with the highest percentage of the sensitivity of 16.9%. Besides that, selectivity tests were conducted to distinguish the existence of Leptospira, Pseudomonas aeruginosa, and Staphylococcus aureus bacteria. These results confirm the potentials of polyaniline metal alloys’ nanocomposite thin films to be used for Leptospira bacteria detection in water. SAGE Publications 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/97600/1/ABSTRACT.pdf Abdullah, Huda and Mustaza, Seri Mastura and Bejo, Siti Khairani and Yahya, Iskandar and Kamal, Noorfazila and Othman, Mohd Hafiz Dzarfan (2021) Identification of Leptospira in water by Fe-Pd-doped polyaniline nanocomposite thin film. Nanomaterials and Nanotechnology, 11. pp. 1-7. ISSN 1847-9804 https://journals.sagepub.com/doi/full/10.1177/18479804211011389 10.1177/18479804211011389
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English
description	Leptospirosis disease was caused by rat urine which contains the genus Leptospira bacteria. In this study, the fabrication of Pd-Fe-doped polyaniline nanocomposite thin films for the determination of the genus Leptospira bacteria thin films has been investigated. Pd-Fe-doped polyaniline nanocomposite thin films were fabricated by sol–gel spin coating method. The electrode sensors were immersed in the Leptospira solution. The resulting materials were investigated using field-emission scanning electron microscopy, atomic force microscopy, transmission electron microscopy, and current–voltage measurement. The atomic force microscopy images show the specific morphology films’ structure for Leptospira detection, whereas the field-emission scanning electron microscopy image shows the irregularity of clump nanoparticles in thin film surfaces. Transmission electron microscopy result shows that metal alloy (Fe-Pd) embedded in the polymer matrix. Current–voltage measurement with and without incubation of the thin film into Leptospira solution was done to show the relationship between concentration bacteria versus current. The result shows that polyaniline-Fe0.4-Pd0.6 nanocomposite thin film has higher sensitivity in detecting Leptospira, where it has performed with the highest percentage of the sensitivity of 16.9%. Besides that, selectivity tests were conducted to distinguish the existence of Leptospira, Pseudomonas aeruginosa, and Staphylococcus aureus bacteria. These results confirm the potentials of polyaniline metal alloys’ nanocomposite thin films to be used for Leptospira bacteria detection in water.
format	Article
author	Abdullah, Huda Mustaza, Seri Mastura Bejo, Siti Khairani Yahya, Iskandar Kamal, Noorfazila Othman, Mohd Hafiz Dzarfan
spellingShingle	Abdullah, Huda Mustaza, Seri Mastura Bejo, Siti Khairani Yahya, Iskandar Kamal, Noorfazila Othman, Mohd Hafiz Dzarfan Identification of Leptospira in water by Fe-Pd-doped polyaniline nanocomposite thin film
author_facet	Abdullah, Huda Mustaza, Seri Mastura Bejo, Siti Khairani Yahya, Iskandar Kamal, Noorfazila Othman, Mohd Hafiz Dzarfan
author_sort	Abdullah, Huda
title	Identification of Leptospira in water by Fe-Pd-doped polyaniline nanocomposite thin film
title_short	Identification of Leptospira in water by Fe-Pd-doped polyaniline nanocomposite thin film
title_full	Identification of Leptospira in water by Fe-Pd-doped polyaniline nanocomposite thin film
title_fullStr	Identification of Leptospira in water by Fe-Pd-doped polyaniline nanocomposite thin film
title_full_unstemmed	Identification of Leptospira in water by Fe-Pd-doped polyaniline nanocomposite thin film
title_sort	identification of leptospira in water by fe-pd-doped polyaniline nanocomposite thin film
publisher	SAGE Publications
publishDate	2021
url	http://psasir.upm.edu.my/id/eprint/97600/1/ABSTRACT.pdf http://psasir.upm.edu.my/id/eprint/97600/ https://journals.sagepub.com/doi/full/10.1177/18479804211011389
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score	13.211869

Identification of Leptospira in water by Fe-Pd-doped polyaniline nanocomposite thin film

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