Staff View: Human Dental Pulp Stem Cells (DPSCs) therapy in rescuing photoreceptors and establishing a sodium iodate-induced retinal degeneration rat model

Human Dental Pulp Stem Cells (DPSCs) therapy in rescuing photoreceptors and establishing a sodium iodate-induced retinal degeneration rat model

Background: Different methods have been used to inject stem cells into the eye for research. We previously explored the intravitreal route. Here, we investigate the efficacy of intravenous and subretinal-transplanted human dental pulp stem cells (DPSCs) in rescuing the photoreceptors of a sodium iod...

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Main Authors:	Lam, Chenshen, Alsaeedi, Hiba Amer, Koh, Avin Ee Hwan, Harun, Mohd Hairul Nizam, Ng, Angela Min Hwei, Mok, Pooi Ling, Chi, D Luu, Kong, Yong Then, Subbiah, Suresh Kumar, Bastion, Mae-Lynn Catherine
Format:	Article
Language:	English
Published:	Springer 2021
Online Access:	http://psasir.upm.edu.my/id/eprint/97565/1/ABSTRACT.pdf http://psasir.upm.edu.my/id/eprint/97565/ https://link.springer.com/article/10.1007/s13770-020-00312-1
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spelling	my.upm.eprints.975652022-07-27T01:46:04Z http://psasir.upm.edu.my/id/eprint/97565/ Human Dental Pulp Stem Cells (DPSCs) therapy in rescuing photoreceptors and establishing a sodium iodate-induced retinal degeneration rat model Lam, Chenshen Alsaeedi, Hiba Amer Koh, Avin Ee Hwan Harun, Mohd Hairul Nizam Ng, Angela Min Hwei Mok, Pooi Ling Chi, D Luu Kong, Yong Then Subbiah, Suresh Kumar Bastion, Mae-Lynn Catherine Background: Different methods have been used to inject stem cells into the eye for research. We previously explored the intravitreal route. Here, we investigate the efficacy of intravenous and subretinal-transplanted human dental pulp stem cells (DPSCs) in rescuing the photoreceptors of a sodium iodate-induced retinal degeneration model. Methods: Three groups of Sprague Dawley rats were used: intervention, vehicle group and negative control groups (n = 6 in each). Intravenous injection of 60 mg/kg sodium iodate (day 0) induced retinal degeneration. On day 4 post-injection of sodium iodate, the rats in the intervention group received intravenous DPSC and subretinal DPSC in the right eye; rats in the vehicle group received subretinal Hank's balance salt solution and intravenous normal saline; while negative control group received nothing. Electroretinogram (ERG) was performed to assess the retinal function at day 0 (baseline), day 4, day 11, day 18, day 26, and day 32. By the end of the study at day 32, the rats were euthanized, and both their enucleated eyes were sent for histology. Results: No significant difference in maximal ERG a-wave (p = 0.107) and b-wave, (p = 0.153) amplitude was seen amongst the experimental groups. However, photopic 30 Hz flicker amplitude of the study eye showed significant differences in the 3 groups (p = 0.032). Within the intervention group, there was an improvement in 30 Hz flicker ERG response of all 6 treated right eyes, which was injected with subretinal DPSC; while the 30 Hz flicker ERG of the non-treated left eyes remained flat. Histology showed improved outer nuclear layer thickness in intervention group; however, findings were not significant compared to the negative and vehicle groups. Conclusion: Combination of subretinal and intravenous injection of DPSCs may have potential to rescue cone function from a NaIO3-induced retinal injury model. Springer 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/97565/1/ABSTRACT.pdf Lam, Chenshen and Alsaeedi, Hiba Amer and Koh, Avin Ee Hwan and Harun, Mohd Hairul Nizam and Ng, Angela Min Hwei and Mok, Pooi Ling and Chi, D Luu and Kong, Yong Then and Subbiah, Suresh Kumar and Bastion, Mae-Lynn Catherine (2021) Human Dental Pulp Stem Cells (DPSCs) therapy in rescuing photoreceptors and establishing a sodium iodate-induced retinal degeneration rat model. Tissue Engineering and Regenerative Medicine, 18 (1). 143 - 154. ISSN 1932-7005 https://link.springer.com/article/10.1007/s13770-020-00312-1 10.1007/s13770-020-00312-1
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English
description	Background: Different methods have been used to inject stem cells into the eye for research. We previously explored the intravitreal route. Here, we investigate the efficacy of intravenous and subretinal-transplanted human dental pulp stem cells (DPSCs) in rescuing the photoreceptors of a sodium iodate-induced retinal degeneration model. Methods: Three groups of Sprague Dawley rats were used: intervention, vehicle group and negative control groups (n = 6 in each). Intravenous injection of 60 mg/kg sodium iodate (day 0) induced retinal degeneration. On day 4 post-injection of sodium iodate, the rats in the intervention group received intravenous DPSC and subretinal DPSC in the right eye; rats in the vehicle group received subretinal Hank's balance salt solution and intravenous normal saline; while negative control group received nothing. Electroretinogram (ERG) was performed to assess the retinal function at day 0 (baseline), day 4, day 11, day 18, day 26, and day 32. By the end of the study at day 32, the rats were euthanized, and both their enucleated eyes were sent for histology. Results: No significant difference in maximal ERG a-wave (p = 0.107) and b-wave, (p = 0.153) amplitude was seen amongst the experimental groups. However, photopic 30 Hz flicker amplitude of the study eye showed significant differences in the 3 groups (p = 0.032). Within the intervention group, there was an improvement in 30 Hz flicker ERG response of all 6 treated right eyes, which was injected with subretinal DPSC; while the 30 Hz flicker ERG of the non-treated left eyes remained flat. Histology showed improved outer nuclear layer thickness in intervention group; however, findings were not significant compared to the negative and vehicle groups. Conclusion: Combination of subretinal and intravenous injection of DPSCs may have potential to rescue cone function from a NaIO3-induced retinal injury model.
format	Article
author	Lam, Chenshen Alsaeedi, Hiba Amer Koh, Avin Ee Hwan Harun, Mohd Hairul Nizam Ng, Angela Min Hwei Mok, Pooi Ling Chi, D Luu Kong, Yong Then Subbiah, Suresh Kumar Bastion, Mae-Lynn Catherine
spellingShingle	Lam, Chenshen Alsaeedi, Hiba Amer Koh, Avin Ee Hwan Harun, Mohd Hairul Nizam Ng, Angela Min Hwei Mok, Pooi Ling Chi, D Luu Kong, Yong Then Subbiah, Suresh Kumar Bastion, Mae-Lynn Catherine Human Dental Pulp Stem Cells (DPSCs) therapy in rescuing photoreceptors and establishing a sodium iodate-induced retinal degeneration rat model
author_facet	Lam, Chenshen Alsaeedi, Hiba Amer Koh, Avin Ee Hwan Harun, Mohd Hairul Nizam Ng, Angela Min Hwei Mok, Pooi Ling Chi, D Luu Kong, Yong Then Subbiah, Suresh Kumar Bastion, Mae-Lynn Catherine
author_sort	Lam, Chenshen
title	Human Dental Pulp Stem Cells (DPSCs) therapy in rescuing photoreceptors and establishing a sodium iodate-induced retinal degeneration rat model
title_short	Human Dental Pulp Stem Cells (DPSCs) therapy in rescuing photoreceptors and establishing a sodium iodate-induced retinal degeneration rat model
title_full	Human Dental Pulp Stem Cells (DPSCs) therapy in rescuing photoreceptors and establishing a sodium iodate-induced retinal degeneration rat model
title_fullStr	Human Dental Pulp Stem Cells (DPSCs) therapy in rescuing photoreceptors and establishing a sodium iodate-induced retinal degeneration rat model
title_full_unstemmed	Human Dental Pulp Stem Cells (DPSCs) therapy in rescuing photoreceptors and establishing a sodium iodate-induced retinal degeneration rat model
title_sort	human dental pulp stem cells (dpscs) therapy in rescuing photoreceptors and establishing a sodium iodate-induced retinal degeneration rat model
publisher	Springer
publishDate	2021
url	http://psasir.upm.edu.my/id/eprint/97565/1/ABSTRACT.pdf http://psasir.upm.edu.my/id/eprint/97565/ https://link.springer.com/article/10.1007/s13770-020-00312-1
_version_	1739829912375656448
score	13.211869

Human Dental Pulp Stem Cells (DPSCs) therapy in rescuing photoreceptors and establishing a sodium iodate-induced retinal degeneration rat model

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