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Extraction and reimmobilization of used commercial lipase from industrial waste

In industrial application, immobilized lipase are typically not reused and served as industrial waste after a certain process is completed. The capacity on the reusability of the spent lipase is not well studied. This current study embarks on reusing the remaining lipase from the spent immobilized e...

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Main Authors:	Mokhtar, Nur Fathiah, Raja Abd Rahman, Raja Noor Zaliha, Sani, Fatimah, Mohamad Ali, Mohd Shukuri
Format:	Article
Language:	English
Published:	Elsevier 2021
Online Access:	http://psasir.upm.edu.my/id/eprint/97241/1/ABSTRACT.pdf http://psasir.upm.edu.my/id/eprint/97241/ https://www.sciencedirect.com/science/article/pii/S0141813021002695?via%3Dihub
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spelling	my.upm.eprints.972412022-09-12T08:47:45Z http://psasir.upm.edu.my/id/eprint/97241/ Extraction and reimmobilization of used commercial lipase from industrial waste Mokhtar, Nur Fathiah Raja Abd Rahman, Raja Noor Zaliha Sani, Fatimah Mohamad Ali, Mohd Shukuri In industrial application, immobilized lipase are typically not reused and served as industrial waste after a certain process is completed. The capacity on the reusability of the spent lipase is not well studied. This current study embarks on reusing the remaining lipase from the spent immobilized enzyme. Active lipases were recovered using a simple reverse micellar extraction (RME). RME is the extraction process of targeted biomolecules using an organic solvent and a surfactant. This method was the first attempt reported on the recovery of the lipase from the used immobilized lipase. RME of the spent lipase was done using the nonionic Triton X-100 surfactant and toluene. Various parameters were optimized to maximize the lipase recovery from the used immobilized lipase. The optimum forward extraction condition was 0.075 M KCl, and backward conditions were at 0.15 M Triton X-100/toluene (pH 6, 2 M KCl) with recovery of 66%. The extracted lipase was immobilized via simple adsorption into the ethanol pretreated carrier. The optimum conditions of immobilization resulted in 96% of the extracted lipase was reimmobilized. The reimmobilized lipase was incubated for 20 h in pH 6 buffer at 50 °C of water bath shaker. The reimmobilized lipase still had 27% residual activity after 18 h of incubation, which higher thermal stability compared to the free lipase. In conclusion, the free lipase was successfully extracted from the spent immobilized lipase and reimmobilized into the new support. It exhibited high thermal stability, and the reusability of the spent lipase will promote continued use of industrial lipase and reduce the cost of the manufacturing process. Elsevier 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/97241/1/ABSTRACT.pdf Mokhtar, Nur Fathiah and Raja Abd Rahman, Raja Noor Zaliha and Sani, Fatimah and Mohamad Ali, Mohd Shukuri (2021) Extraction and reimmobilization of used commercial lipase from industrial waste. International Journal of Biological Macromolecules, 176. 413 - 423. ISSN 0141-8130; ESSN: 1879-0003 https://www.sciencedirect.com/science/article/pii/S0141813021002695?via%3Dihub 10.1016/j.ijbiomac.2021.02.001
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English
description	In industrial application, immobilized lipase are typically not reused and served as industrial waste after a certain process is completed. The capacity on the reusability of the spent lipase is not well studied. This current study embarks on reusing the remaining lipase from the spent immobilized enzyme. Active lipases were recovered using a simple reverse micellar extraction (RME). RME is the extraction process of targeted biomolecules using an organic solvent and a surfactant. This method was the first attempt reported on the recovery of the lipase from the used immobilized lipase. RME of the spent lipase was done using the nonionic Triton X-100 surfactant and toluene. Various parameters were optimized to maximize the lipase recovery from the used immobilized lipase. The optimum forward extraction condition was 0.075 M KCl, and backward conditions were at 0.15 M Triton X-100/toluene (pH 6, 2 M KCl) with recovery of 66%. The extracted lipase was immobilized via simple adsorption into the ethanol pretreated carrier. The optimum conditions of immobilization resulted in 96% of the extracted lipase was reimmobilized. The reimmobilized lipase was incubated for 20 h in pH 6 buffer at 50 °C of water bath shaker. The reimmobilized lipase still had 27% residual activity after 18 h of incubation, which higher thermal stability compared to the free lipase. In conclusion, the free lipase was successfully extracted from the spent immobilized lipase and reimmobilized into the new support. It exhibited high thermal stability, and the reusability of the spent lipase will promote continued use of industrial lipase and reduce the cost of the manufacturing process.
format	Article
author	Mokhtar, Nur Fathiah Raja Abd Rahman, Raja Noor Zaliha Sani, Fatimah Mohamad Ali, Mohd Shukuri
spellingShingle	Mokhtar, Nur Fathiah Raja Abd Rahman, Raja Noor Zaliha Sani, Fatimah Mohamad Ali, Mohd Shukuri Extraction and reimmobilization of used commercial lipase from industrial waste
author_facet	Mokhtar, Nur Fathiah Raja Abd Rahman, Raja Noor Zaliha Sani, Fatimah Mohamad Ali, Mohd Shukuri
author_sort	Mokhtar, Nur Fathiah
title	Extraction and reimmobilization of used commercial lipase from industrial waste
title_short	Extraction and reimmobilization of used commercial lipase from industrial waste
title_full	Extraction and reimmobilization of used commercial lipase from industrial waste
title_fullStr	Extraction and reimmobilization of used commercial lipase from industrial waste
title_full_unstemmed	Extraction and reimmobilization of used commercial lipase from industrial waste
title_sort	extraction and reimmobilization of used commercial lipase from industrial waste
publisher	Elsevier
publishDate	2021
url	http://psasir.upm.edu.my/id/eprint/97241/1/ABSTRACT.pdf http://psasir.upm.edu.my/id/eprint/97241/ https://www.sciencedirect.com/science/article/pii/S0141813021002695?via%3Dihub
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score	13.211869

Extraction and reimmobilization of used commercial lipase from industrial waste

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