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Evaluation of effect of ethanol extraction of Graptophyllum pictum on Zebrafish (Danio rerio) embryo model through toxicity assay assessment

Graptophyllum pictum is a medicinal plant that helps to cure different forms of disease due to the availability of beneficial phytochemicals such as flavonoids, steroids, alkaloids, saponins and glycosides. The purpose of this research was to evaluate the toxicity effect of G. pictum extract on zebr...

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Main Authors: Zainal Abidin, Azrina, Visepomaran, Subashini, Balan, Santhra Segaran, Bahari, Hasnah
Format: Article
Language:English
Published: ClinMed International Library 2021
Online Access:http://psasir.upm.edu.my/id/eprint/97155/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/97155/
https://clinmedjournals.org/articles/ijtra/international-journal-of-toxicology-and-risk-assessment-ijtra-7-040.php?jid=ijtra
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spelling my.upm.eprints.971552022-09-14T01:30:21Z http://psasir.upm.edu.my/id/eprint/97155/ Evaluation of effect of ethanol extraction of Graptophyllum pictum on Zebrafish (Danio rerio) embryo model through toxicity assay assessment Zainal Abidin, Azrina Visepomaran, Subashini Balan, Santhra Segaran Bahari, Hasnah Graptophyllum pictum is a medicinal plant that helps to cure different forms of disease due to the availability of beneficial phytochemicals such as flavonoids, steroids, alkaloids, saponins and glycosides. The purpose of this research was to evaluate the toxicity effect of G. pictum extract on zebrafish embryos (Danio rerio) at different concentrations. G. pictum was extracted using the ethanol method. A toxicity test was done by exposing the Danio rerio embryo to the G. pictum extraction at different concentrations (0.244-1000 μg/ml) for 24, 48, 72 and 96 hours. The survival rate, hatching rate, heartbeat rate, scoliosis rate and melanin pigmentation were observed. Data were analyzed using SPSS version 27. The value of LC50 was calculated. Result showed that the LC50, value of G. pictum is 7.662 μg/ml. No hatching was observed at higher concentrations (31.25-1000 μg/ml) while the hatch ability of Danio rerio embryos was observed at lower concentrations (0.244-1.953 μg/ml). Scoliosis of zebrafish larvae was not present at all concentrations. The heartbeat of the zebrafish larvae treated with G. pictum extract was within a normal range from 120-180 beats per minute at a lower concentration. Melanin pigmentation was detected at 48, 72 and 96 hours post-fertilization and is normally absent at 24 hours post-fertilization. As a conclusion, G. pictum extract stills exhibit a mild toxicity effect in higher concentrations when it was evaluated on zebrafish embryos. ClinMed International Library 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/97155/1/ABSTRACT.pdf Zainal Abidin, Azrina and Visepomaran, Subashini and Balan, Santhra Segaran and Bahari, Hasnah (2021) Evaluation of effect of ethanol extraction of Graptophyllum pictum on Zebrafish (Danio rerio) embryo model through toxicity assay assessment. Journal of Toxicology and Risk Assessment, 7 (1). pp. 1-8. ISSN 2572-4061 https://clinmedjournals.org/articles/ijtra/international-journal-of-toxicology-and-risk-assessment-ijtra-7-040.php?jid=ijtra 10.23937/2572-4061.1510040
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Graptophyllum pictum is a medicinal plant that helps to cure different forms of disease due to the availability of beneficial phytochemicals such as flavonoids, steroids, alkaloids, saponins and glycosides. The purpose of this research was to evaluate the toxicity effect of G. pictum extract on zebrafish embryos (Danio rerio) at different concentrations. G. pictum was extracted using the ethanol method. A toxicity test was done by exposing the Danio rerio embryo to the G. pictum extraction at different concentrations (0.244-1000 μg/ml) for 24, 48, 72 and 96 hours. The survival rate, hatching rate, heartbeat rate, scoliosis rate and melanin pigmentation were observed. Data were analyzed using SPSS version 27. The value of LC50 was calculated. Result showed that the LC50, value of G. pictum is 7.662 μg/ml. No hatching was observed at higher concentrations (31.25-1000 μg/ml) while the hatch ability of Danio rerio embryos was observed at lower concentrations (0.244-1.953 μg/ml). Scoliosis of zebrafish larvae was not present at all concentrations. The heartbeat of the zebrafish larvae treated with G. pictum extract was within a normal range from 120-180 beats per minute at a lower concentration. Melanin pigmentation was detected at 48, 72 and 96 hours post-fertilization and is normally absent at 24 hours post-fertilization. As a conclusion, G. pictum extract stills exhibit a mild toxicity effect in higher concentrations when it was evaluated on zebrafish embryos.
format Article
author Zainal Abidin, Azrina
Visepomaran, Subashini
Balan, Santhra Segaran
Bahari, Hasnah
spellingShingle Zainal Abidin, Azrina
Visepomaran, Subashini
Balan, Santhra Segaran
Bahari, Hasnah
Evaluation of effect of ethanol extraction of Graptophyllum pictum on Zebrafish (Danio rerio) embryo model through toxicity assay assessment
author_facet Zainal Abidin, Azrina
Visepomaran, Subashini
Balan, Santhra Segaran
Bahari, Hasnah
author_sort Zainal Abidin, Azrina
title Evaluation of effect of ethanol extraction of Graptophyllum pictum on Zebrafish (Danio rerio) embryo model through toxicity assay assessment
title_short Evaluation of effect of ethanol extraction of Graptophyllum pictum on Zebrafish (Danio rerio) embryo model through toxicity assay assessment
title_full Evaluation of effect of ethanol extraction of Graptophyllum pictum on Zebrafish (Danio rerio) embryo model through toxicity assay assessment
title_fullStr Evaluation of effect of ethanol extraction of Graptophyllum pictum on Zebrafish (Danio rerio) embryo model through toxicity assay assessment
title_full_unstemmed Evaluation of effect of ethanol extraction of Graptophyllum pictum on Zebrafish (Danio rerio) embryo model through toxicity assay assessment
title_sort evaluation of effect of ethanol extraction of graptophyllum pictum on zebrafish (danio rerio) embryo model through toxicity assay assessment
publisher ClinMed International Library
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/97155/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/97155/
https://clinmedjournals.org/articles/ijtra/international-journal-of-toxicology-and-risk-assessment-ijtra-7-040.php?jid=ijtra
_version_ 1744355307853709312
score 13.211869

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