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Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals

The current cut-off value for thalassaemia screening is based on a mean corpuscular haemoglobin (MCH) level of less than 27 pg. Samples with MCH < 27 pg require additional confirmatory testing, while samples with MCH > 27 pg are considered normal. However, there is a possibility of missing som...

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Main Authors: Mohd. Yusoff, Mohd. Fauzi, Mohd Tohit, Eusni Rahayu, Hashim, Hafizah, Seman, Zainina
Format: Article
Language:English
Published: Universiti Putra Malaysia 2021
Online Access:http://psasir.upm.edu.my/id/eprint/97154/3/2021120708140602_MJMHS_0524.pdf
http://psasir.upm.edu.my/id/eprint/97154/
https://medic.upm.edu.my/upload/dokumen/2021120708140602_MJMHS_0524.pdf
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spelling my.upm.eprints.971542022-09-14T01:29:32Z http://psasir.upm.edu.my/id/eprint/97154/ Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals Mohd. Yusoff, Mohd. Fauzi Mohd Tohit, Eusni Rahayu Hashim, Hafizah Seman, Zainina The current cut-off value for thalassaemia screening is based on a mean corpuscular haemoglobin (MCH) level of less than 27 pg. Samples with MCH < 27 pg require additional confirmatory testing, while samples with MCH > 27 pg are considered normal. However, there is a possibility of missing some types of haemoglobinopathy such as haemoglobin (Hb) E trait, by using this cut-off value. This study aimed to evaluate the dichloroindophenol (DCIP) test for Hb E carriers within healthy individuals. Methods: In total, 200 leftover blood samples with normal full blood count (FBC) results and MCH ≥ 27 pg were collected from students who were involved in the thalassaemia screening program. Blood samples were screened for Hb E by the DCIP test and subjected to haemoglobin analysis using capillary electrophoresis (CE) and gel electrophoresis. Results: Of the 200 leftover blood samples with normal FBC indices, 10 were positive for the DCIP test of which 8 had Hb E trait and 2 had Hb Constant Spring. The DCIP test showed 100% sensitivity and 98.96% specificity with a 100% negative predictive value (NPV) and an 80.0 % positive predictive value (PPV). Conclusion: The DCIP test was an excellent screening tool that, when combined with FBC parameters can produce better results, particularly in areas with a high Hb E incidence. Universiti Putra Malaysia 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/97154/3/2021120708140602_MJMHS_0524.pdf Mohd. Yusoff, Mohd. Fauzi and Mohd Tohit, Eusni Rahayu and Hashim, Hafizah and Seman, Zainina (2021) Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals. Malaysian Journal of Medicine and Health Sciences, 17 (suppl.10). pp. 10-13. ISSN 2636-9346 https://medic.upm.edu.my/upload/dokumen/2021120708140602_MJMHS_0524.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The current cut-off value for thalassaemia screening is based on a mean corpuscular haemoglobin (MCH) level of less than 27 pg. Samples with MCH < 27 pg require additional confirmatory testing, while samples with MCH > 27 pg are considered normal. However, there is a possibility of missing some types of haemoglobinopathy such as haemoglobin (Hb) E trait, by using this cut-off value. This study aimed to evaluate the dichloroindophenol (DCIP) test for Hb E carriers within healthy individuals. Methods: In total, 200 leftover blood samples with normal full blood count (FBC) results and MCH ≥ 27 pg were collected from students who were involved in the thalassaemia screening program. Blood samples were screened for Hb E by the DCIP test and subjected to haemoglobin analysis using capillary electrophoresis (CE) and gel electrophoresis. Results: Of the 200 leftover blood samples with normal FBC indices, 10 were positive for the DCIP test of which 8 had Hb E trait and 2 had Hb Constant Spring. The DCIP test showed 100% sensitivity and 98.96% specificity with a 100% negative predictive value (NPV) and an 80.0 % positive predictive value (PPV). Conclusion: The DCIP test was an excellent screening tool that, when combined with FBC parameters can produce better results, particularly in areas with a high Hb E incidence.
format Article
author Mohd. Yusoff, Mohd. Fauzi
Mohd Tohit, Eusni Rahayu
Hashim, Hafizah
Seman, Zainina
spellingShingle Mohd. Yusoff, Mohd. Fauzi
Mohd Tohit, Eusni Rahayu
Hashim, Hafizah
Seman, Zainina
Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals
author_facet Mohd. Yusoff, Mohd. Fauzi
Mohd Tohit, Eusni Rahayu
Hashim, Hafizah
Seman, Zainina
author_sort Mohd. Yusoff, Mohd. Fauzi
title Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals
title_short Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals
title_full Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals
title_fullStr Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals
title_full_unstemmed Evaluation of Dichlorophenolindophenol (DCIP) test for Haemoglobin E (Hb E) in normal red cell indices individuals
title_sort evaluation of dichlorophenolindophenol (dcip) test for haemoglobin e (hb e) in normal red cell indices individuals
publisher Universiti Putra Malaysia
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/97154/3/2021120708140602_MJMHS_0524.pdf
http://psasir.upm.edu.my/id/eprint/97154/
https://medic.upm.edu.my/upload/dokumen/2021120708140602_MJMHS_0524.pdf
_version_ 1744355307711102976
score 13.211869

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