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Enzymatically synthesised fructooligosaccharides from sugarcane syrup modulate the composition and short-chain fatty acid production of the human intestinal microbiota

Fructooligosaccharides can be produced by direct enzymatic conversion from sucrose-rich sugarcane syrup (SS) consisting of 58.93% sucrose yielding 21.28 g FOS/100 g sucrose. This study evaluated the prebiotic effect of unpurified/purified SS containing FOS for the modulation of the human intestinal...

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Main Authors: Azhari, Siti Hajar, Abd Rahim, Muhamad Hafiz, Sarbini, Shahrul Razid, Muhialdin, Belal J., Olusegun, Lasekan, Saari, Nazamid
Format: Article
Language:English
Published: Elsevier 2021
Online Access:http://psasir.upm.edu.my/id/eprint/97121/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/97121/
https://www.sciencedirect.com/science/article/abs/pii/S0963996921005767
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spelling my.upm.eprints.971212022-10-15T15:42:58Z http://psasir.upm.edu.my/id/eprint/97121/ Enzymatically synthesised fructooligosaccharides from sugarcane syrup modulate the composition and short-chain fatty acid production of the human intestinal microbiota Azhari, Siti Hajar Abd Rahim, Muhamad Hafiz Sarbini, Shahrul Razid Muhialdin, Belal J. Olusegun, Lasekan Saari, Nazamid Fructooligosaccharides can be produced by direct enzymatic conversion from sucrose-rich sugarcane syrup (SS) consisting of 58.93% sucrose yielding 21.28 g FOS/100 g sucrose. This study evaluated the prebiotic effect of unpurified/purified SS containing FOS for the modulation of the human intestinal microbial composition and short-chain fatty acid production. The unpurified and purified FOS substrates, which were a mixture of 1-kestose, nystose and 1F-fructosylnystose, were supplemented into human faecal culture using a pH-controlled batch fermentation system and significantly increased the Bifidobacterium counts after 5 h fermentation, while Bacteroides/Prevotella counts were highest throughout 24 h fermentation. Meanwhile, Lactobacillus/Enterococcus exhibited a slight increase after 5 h fermentation before reaching a plateau afterwards. The steady Bacteroides/Prevotella growth and increased Bifidobacterium population promoted an increase in the production of short-chain fatty acids acetate (58 ± 2.70 mM), propionate (9.19 ± 5.94 mM) and butyrate (7.15 ± 2.28 mM). These results provide evidence that representative gut microbiota could utilise the enzymatically synthesised FOS to generate short-chain fatty acids as metabolites in pH-controlled conditions, thus FOS from SS are a potential prebiotic ingredient for foods and health drinks. Elsevier 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/97121/1/ABSTRACT.pdf Azhari, Siti Hajar and Abd Rahim, Muhamad Hafiz and Sarbini, Shahrul Razid and Muhialdin, Belal J. and Olusegun, Lasekan and Saari, Nazamid (2021) Enzymatically synthesised fructooligosaccharides from sugarcane syrup modulate the composition and short-chain fatty acid production of the human intestinal microbiota. Food Research International, 149. art. no. 110677. pp. 1-9. ISSN 0963-9969 https://www.sciencedirect.com/science/article/abs/pii/S0963996921005767 10.1016/j.foodres.2021.110677
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Fructooligosaccharides can be produced by direct enzymatic conversion from sucrose-rich sugarcane syrup (SS) consisting of 58.93% sucrose yielding 21.28 g FOS/100 g sucrose. This study evaluated the prebiotic effect of unpurified/purified SS containing FOS for the modulation of the human intestinal microbial composition and short-chain fatty acid production. The unpurified and purified FOS substrates, which were a mixture of 1-kestose, nystose and 1F-fructosylnystose, were supplemented into human faecal culture using a pH-controlled batch fermentation system and significantly increased the Bifidobacterium counts after 5 h fermentation, while Bacteroides/Prevotella counts were highest throughout 24 h fermentation. Meanwhile, Lactobacillus/Enterococcus exhibited a slight increase after 5 h fermentation before reaching a plateau afterwards. The steady Bacteroides/Prevotella growth and increased Bifidobacterium population promoted an increase in the production of short-chain fatty acids acetate (58 ± 2.70 mM), propionate (9.19 ± 5.94 mM) and butyrate (7.15 ± 2.28 mM). These results provide evidence that representative gut microbiota could utilise the enzymatically synthesised FOS to generate short-chain fatty acids as metabolites in pH-controlled conditions, thus FOS from SS are a potential prebiotic ingredient for foods and health drinks.
format Article
author Azhari, Siti Hajar
Abd Rahim, Muhamad Hafiz
Sarbini, Shahrul Razid
Muhialdin, Belal J.
Olusegun, Lasekan
Saari, Nazamid
spellingShingle Azhari, Siti Hajar
Abd Rahim, Muhamad Hafiz
Sarbini, Shahrul Razid
Muhialdin, Belal J.
Olusegun, Lasekan
Saari, Nazamid
Enzymatically synthesised fructooligosaccharides from sugarcane syrup modulate the composition and short-chain fatty acid production of the human intestinal microbiota
author_facet Azhari, Siti Hajar
Abd Rahim, Muhamad Hafiz
Sarbini, Shahrul Razid
Muhialdin, Belal J.
Olusegun, Lasekan
Saari, Nazamid
author_sort Azhari, Siti Hajar
title Enzymatically synthesised fructooligosaccharides from sugarcane syrup modulate the composition and short-chain fatty acid production of the human intestinal microbiota
title_short Enzymatically synthesised fructooligosaccharides from sugarcane syrup modulate the composition and short-chain fatty acid production of the human intestinal microbiota
title_full Enzymatically synthesised fructooligosaccharides from sugarcane syrup modulate the composition and short-chain fatty acid production of the human intestinal microbiota
title_fullStr Enzymatically synthesised fructooligosaccharides from sugarcane syrup modulate the composition and short-chain fatty acid production of the human intestinal microbiota
title_full_unstemmed Enzymatically synthesised fructooligosaccharides from sugarcane syrup modulate the composition and short-chain fatty acid production of the human intestinal microbiota
title_sort enzymatically synthesised fructooligosaccharides from sugarcane syrup modulate the composition and short-chain fatty acid production of the human intestinal microbiota
publisher Elsevier
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/97121/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/97121/
https://www.sciencedirect.com/science/article/abs/pii/S0963996921005767
_version_ 1748182298430275584
score 13.211869

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