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Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches

Southern highbush blueberry (interspecific hybrids of Vaccinium corymbosum L.) is cultivated near wild V. corymbosum as well as closely related species in Florida, USA. The expansion of blueberry cultivation into new areas in Florida and deployment of new cultivars containing viruses can potentially...

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Main Authors: Saad, Norsazilawati, Olmstead, James W., Varsani, Arvind, Polston, Jane Elizabeth, Jones, Jeffery, Folimonova, Svetlana Y., Harmon, Philip F.
Format: Article
Language:English
Published: Multidisciplinary Digital Publishing Institute 2021
Online Access:http://psasir.upm.edu.my/id/eprint/96754/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/96754/
https://www.mdpi.com/1999-4915/13/6/1165
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spelling my.upm.eprints.967542022-12-01T07:30:50Z http://psasir.upm.edu.my/id/eprint/96754/ Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches Saad, Norsazilawati Olmstead, James W. Varsani, Arvind Polston, Jane Elizabeth Jones, Jeffery Folimonova, Svetlana Y. Harmon, Philip F. Southern highbush blueberry (interspecific hybrids of Vaccinium corymbosum L.) is cultivated near wild V. corymbosum as well as closely related species in Florida, USA. The expansion of blueberry cultivation into new areas in Florida and deployment of new cultivars containing viruses can potentially increase the diversity of viruses in wild and cultivated V. corymbosum. In this study, viral diversity in wild and cultivated blueberries (V. corymbosum) is described using a metagenomic approach. RNA viromes from V. corymbosum plants collected from six locations (two cultivated and four wild) in North Central Florida were generated by high throughput sequencing (HTS) and analyzed using a bioinformatic analysis pipeline. De novo assembled contigs obtained from viromes of both commercial and wild sites produced sequences with similarities to plant virus species from a diverse range of families (Amalgaviridae, Caulimoviridae, Endornaviridae, Ophioviridae, Phenuiviridae, and Virgaviridae). In addition, this study has enabled the identification of blueberry latent virus (BlLV) and blueberry mosaic associated ophiovirus (BlMaV) for the first time in Florida, as well as a tentative novel tepovirus (blueberry virus T) (BlVT) in blueberry. To the best of our knowledge, this is the first study that compares viral diversity in wild and cultivated blueberry using a metagenomic approach. Multidisciplinary Digital Publishing Institute 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/96754/1/ABSTRACT.pdf Saad, Norsazilawati and Olmstead, James W. and Varsani, Arvind and Polston, Jane Elizabeth and Jones, Jeffery and Folimonova, Svetlana Y. and Harmon, Philip F. (2021) Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches. Viruses-Basel, 13 (6). pp. 1-16. ISSN 1999-4915 https://www.mdpi.com/1999-4915/13/6/1165 10.3390/v13061165
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Southern highbush blueberry (interspecific hybrids of Vaccinium corymbosum L.) is cultivated near wild V. corymbosum as well as closely related species in Florida, USA. The expansion of blueberry cultivation into new areas in Florida and deployment of new cultivars containing viruses can potentially increase the diversity of viruses in wild and cultivated V. corymbosum. In this study, viral diversity in wild and cultivated blueberries (V. corymbosum) is described using a metagenomic approach. RNA viromes from V. corymbosum plants collected from six locations (two cultivated and four wild) in North Central Florida were generated by high throughput sequencing (HTS) and analyzed using a bioinformatic analysis pipeline. De novo assembled contigs obtained from viromes of both commercial and wild sites produced sequences with similarities to plant virus species from a diverse range of families (Amalgaviridae, Caulimoviridae, Endornaviridae, Ophioviridae, Phenuiviridae, and Virgaviridae). In addition, this study has enabled the identification of blueberry latent virus (BlLV) and blueberry mosaic associated ophiovirus (BlMaV) for the first time in Florida, as well as a tentative novel tepovirus (blueberry virus T) (BlVT) in blueberry. To the best of our knowledge, this is the first study that compares viral diversity in wild and cultivated blueberry using a metagenomic approach.
format Article
author Saad, Norsazilawati
Olmstead, James W.
Varsani, Arvind
Polston, Jane Elizabeth
Jones, Jeffery
Folimonova, Svetlana Y.
Harmon, Philip F.
spellingShingle Saad, Norsazilawati
Olmstead, James W.
Varsani, Arvind
Polston, Jane Elizabeth
Jones, Jeffery
Folimonova, Svetlana Y.
Harmon, Philip F.
Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches
author_facet Saad, Norsazilawati
Olmstead, James W.
Varsani, Arvind
Polston, Jane Elizabeth
Jones, Jeffery
Folimonova, Svetlana Y.
Harmon, Philip F.
author_sort Saad, Norsazilawati
title Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches
title_short Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches
title_full Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches
title_fullStr Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches
title_full_unstemmed Discovery of known and novel viruses in wild and cultivated blueberry in Florida through viral metagenomic approaches
title_sort discovery of known and novel viruses in wild and cultivated blueberry in florida through viral metagenomic approaches
publisher Multidisciplinary Digital Publishing Institute
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/96754/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/96754/
https://www.mdpi.com/1999-4915/13/6/1165
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score 13.211869

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