Staff View: Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor-based gold nanoparticles

Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor-based gold nanoparticles

The E6 region has higher protuberant probability annealing than consensus probe focusing on another region in the human papillomavirus (HPV) genome in terms of detection and screening method. Here, we designed the first multiple virus single-stranded deoxyribonucleic acid (ssDNA) for multiple detect...

Full description

Saved in:

Bibliographic Details
Main Authors:	Jaapar, F. Nadhirah, Parmin, Nor Azizah, A. Halim, N. Hamidah, Hashim, Uda, Gopinath, Subash Chandra Bose, Halim, F. Syakirah, A. Rahim, Ruslinda, Voon, C. H., M. N., Afnan Uda, Sharifah Nadzirah, Rejali, Zulida, Mohd Jamil, Amilia Afzan, Zakaria, Iffah Izzati
Format:	Article
Published:	Wiley 2021
Online Access:	http://psasir.upm.edu.my/id/eprint/96625/ https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bab.2260
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.upm.eprints.96625
record_format	eprints
spelling	my.upm.eprints.966252023-02-01T07:45:50Z http://psasir.upm.edu.my/id/eprint/96625/ Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor-based gold nanoparticles Jaapar, F. Nadhirah Parmin, Nor Azizah A. Halim, N. Hamidah Hashim, Uda Gopinath, Subash Chandra Bose Halim, F. Syakirah A. Rahim, Ruslinda Voon, C. H. M. N., Afnan Uda Sharifah Nadzirah Rejali, Zulida Mohd Jamil, Amilia Afzan Zakaria, Iffah Izzati The E6 region has higher protuberant probability annealing than consensus probe focusing on another region in the human papillomavirus (HPV) genome in terms of detection and screening method. Here, we designed the first multiple virus single-stranded deoxyribonucleic acid (ssDNA) for multiple detections in an early phase of screening for cervical cancer in the E6 region and became a fundamental evolution of detection electrochemical HPV biosensor. Gene profiling of the virus ssDNA sequences has been carried by high-end bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST), and Clustal OMEGA in a row. The output from bioinformatics tools resulted in 100% of similarities between our virus ssDNA probe and HPV complete genome in the databases. The cross-validation between HPV genome and our designed virus ssDNA provided high specificity and selectivity during screening methods compared with Pap smear. The DNA probe for HPV 18, 5' COOH-GAT CCA GAA GGT ACA GAC GGG GAG GGC ACG 3', while 5'COOH-GGG CGC TGT GCA GTG TGT TGG AGA CCC CGA3' as DNA probe for HPV 58 designed with 66.77% guanine (G) and cytosine (C) content for both. Our virus ssDNA probe for the HPV biosensor promises high sensitivity, specificity, selectivity, repeatability, low fluid consumption, and will be useful in mini-size diagnostic devices for cervical cancer detection. Wiley 2021 Article PeerReviewed Jaapar, F. Nadhirah and Parmin, Nor Azizah and A. Halim, N. Hamidah and Hashim, Uda and Gopinath, Subash Chandra Bose and Halim, F. Syakirah and A. Rahim, Ruslinda and Voon, C. H. and M. N., Afnan Uda and Sharifah Nadzirah and Rejali, Zulida and Mohd Jamil, Amilia Afzan and Zakaria, Iffah Izzati (2021) Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor-based gold nanoparticles. Biotechnology and Applied Biochemistry, 69 (5). pp. 1966-1983. ISSN 0885-4513; ESSN: 1470-8744 https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bab.2260 10.1002/bab.2260
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	The E6 region has higher protuberant probability annealing than consensus probe focusing on another region in the human papillomavirus (HPV) genome in terms of detection and screening method. Here, we designed the first multiple virus single-stranded deoxyribonucleic acid (ssDNA) for multiple detections in an early phase of screening for cervical cancer in the E6 region and became a fundamental evolution of detection electrochemical HPV biosensor. Gene profiling of the virus ssDNA sequences has been carried by high-end bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST), and Clustal OMEGA in a row. The output from bioinformatics tools resulted in 100% of similarities between our virus ssDNA probe and HPV complete genome in the databases. The cross-validation between HPV genome and our designed virus ssDNA provided high specificity and selectivity during screening methods compared with Pap smear. The DNA probe for HPV 18, 5' COOH-GAT CCA GAA GGT ACA GAC GGG GAG GGC ACG 3', while 5'COOH-GGG CGC TGT GCA GTG TGT TGG AGA CCC CGA3' as DNA probe for HPV 58 designed with 66.77% guanine (G) and cytosine (C) content for both. Our virus ssDNA probe for the HPV biosensor promises high sensitivity, specificity, selectivity, repeatability, low fluid consumption, and will be useful in mini-size diagnostic devices for cervical cancer detection.
format	Article
author	Jaapar, F. Nadhirah Parmin, Nor Azizah A. Halim, N. Hamidah Hashim, Uda Gopinath, Subash Chandra Bose Halim, F. Syakirah A. Rahim, Ruslinda Voon, C. H. M. N., Afnan Uda Sharifah Nadzirah Rejali, Zulida Mohd Jamil, Amilia Afzan Zakaria, Iffah Izzati
spellingShingle	Jaapar, F. Nadhirah Parmin, Nor Azizah A. Halim, N. Hamidah Hashim, Uda Gopinath, Subash Chandra Bose Halim, F. Syakirah A. Rahim, Ruslinda Voon, C. H. M. N., Afnan Uda Sharifah Nadzirah Rejali, Zulida Mohd Jamil, Amilia Afzan Zakaria, Iffah Izzati Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor-based gold nanoparticles
author_facet	Jaapar, F. Nadhirah Parmin, Nor Azizah A. Halim, N. Hamidah Hashim, Uda Gopinath, Subash Chandra Bose Halim, F. Syakirah A. Rahim, Ruslinda Voon, C. H. M. N., Afnan Uda Sharifah Nadzirah Rejali, Zulida Mohd Jamil, Amilia Afzan Zakaria, Iffah Izzati
author_sort	Jaapar, F. Nadhirah
title	Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor-based gold nanoparticles
title_short	Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor-based gold nanoparticles
title_full	Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor-based gold nanoparticles
title_fullStr	Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor-based gold nanoparticles
title_full_unstemmed	Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor-based gold nanoparticles
title_sort	designing dna probe from hpv 18 and 58 in the e6 region for sensing element in the development of genosensor-based gold nanoparticles
publisher	Wiley
publishDate	2021
url	http://psasir.upm.edu.my/id/eprint/96625/ https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bab.2260
_version_	1756685802057760768
score	13.211869

Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor-based gold nanoparticles

Similar Items