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Co-inoculation of aflatoxigenic and non-aflatoxigenic strains of aspergillus flavus to assess the efficacy of non-aflatoxigenic strains in growth inhibition and aflatoxin b1 reduction

The pre-harvest biocontrol approach currently used includes laboratory inoculations using non-aflatoxigenic strains of Aspergillus flavus. This strategy effectively suppresses the indigenous aflatoxigenic strains and reduces aflatoxin accumulation in sweetcorn. The current in vitro study’s main obje...

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Main Authors: Khan, Rahim, Mohamad Ghazali, Farinazleen, Mahyudin, Nor Ainy, Putra Samsudin, Nik Iskandar
Format: Article
Published: Multidisciplinary Digital Publishing Institute 2021
Online Access:http://psasir.upm.edu.my/id/eprint/96428/
https://www.mdpi.com/2077-0472/11/3/198
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spelling my.upm.eprints.964282023-02-10T01:53:47Z http://psasir.upm.edu.my/id/eprint/96428/ Co-inoculation of aflatoxigenic and non-aflatoxigenic strains of aspergillus flavus to assess the efficacy of non-aflatoxigenic strains in growth inhibition and aflatoxin b1 reduction Khan, Rahim Mohamad Ghazali, Farinazleen Mahyudin, Nor Ainy Putra Samsudin, Nik Iskandar The pre-harvest biocontrol approach currently used includes laboratory inoculations using non-aflatoxigenic strains of Aspergillus flavus. This strategy effectively suppresses the indigenous aflatoxigenic strains and reduces aflatoxin accumulation in sweetcorn. The current in vitro study’s main objective is to determine the diametric growth rates of both Aflatoxin (AF)+ and AF− strains and improve the understanding of competitive relationships among these strains in sweetcorn (Zea mays). Sweetcorn kernels inoculated with AF+ strains only, AF− strains only, and co-inoculated with AF+ + AF− strains were investigated for aflatoxin concentrations. The diametric growth results revealed that growth rates of AF− strains at 25 and 30 °C were much greater than AF+ strains, which was in line with previous studies. The in vitro findings showed that the AKR5− and AKL34− biocontrol strains effectively inhibited the colony propagation and subsequent AFB1 contamination (up to 79%) of AF+ strains. On the other hand, the AKR1− and AKL35− were least effective in reducing AFB1 contents only by 58% and 60%, respectively. There was a significant difference (p < 0.05) in the reduction of AFB1 contents achieved by AF− strains of A. flavus. The findings of the present study indicated the reduction in AFB1 with population expressions of AF+ strains by the AF− strains and supports the notion of competitive exclusion through vigorous development and propagation of the non-aflatoxigenic fungi. Multidisciplinary Digital Publishing Institute 2021 Article PeerReviewed Khan, Rahim and Mohamad Ghazali, Farinazleen and Mahyudin, Nor Ainy and Putra Samsudin, Nik Iskandar (2021) Co-inoculation of aflatoxigenic and non-aflatoxigenic strains of aspergillus flavus to assess the efficacy of non-aflatoxigenic strains in growth inhibition and aflatoxin b1 reduction. Agriculture-Basel, 11 (3). art. no. 198. pp. 1-11. ISSN 2077--0472 https://www.mdpi.com/2077-0472/11/3/198 10.3390/agriculture11030198
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description The pre-harvest biocontrol approach currently used includes laboratory inoculations using non-aflatoxigenic strains of Aspergillus flavus. This strategy effectively suppresses the indigenous aflatoxigenic strains and reduces aflatoxin accumulation in sweetcorn. The current in vitro study’s main objective is to determine the diametric growth rates of both Aflatoxin (AF)+ and AF− strains and improve the understanding of competitive relationships among these strains in sweetcorn (Zea mays). Sweetcorn kernels inoculated with AF+ strains only, AF− strains only, and co-inoculated with AF+ + AF− strains were investigated for aflatoxin concentrations. The diametric growth results revealed that growth rates of AF− strains at 25 and 30 °C were much greater than AF+ strains, which was in line with previous studies. The in vitro findings showed that the AKR5− and AKL34− biocontrol strains effectively inhibited the colony propagation and subsequent AFB1 contamination (up to 79%) of AF+ strains. On the other hand, the AKR1− and AKL35− were least effective in reducing AFB1 contents only by 58% and 60%, respectively. There was a significant difference (p < 0.05) in the reduction of AFB1 contents achieved by AF− strains of A. flavus. The findings of the present study indicated the reduction in AFB1 with population expressions of AF+ strains by the AF− strains and supports the notion of competitive exclusion through vigorous development and propagation of the non-aflatoxigenic fungi.
format Article
author Khan, Rahim
Mohamad Ghazali, Farinazleen
Mahyudin, Nor Ainy
Putra Samsudin, Nik Iskandar
spellingShingle Khan, Rahim
Mohamad Ghazali, Farinazleen
Mahyudin, Nor Ainy
Putra Samsudin, Nik Iskandar
Co-inoculation of aflatoxigenic and non-aflatoxigenic strains of aspergillus flavus to assess the efficacy of non-aflatoxigenic strains in growth inhibition and aflatoxin b1 reduction
author_facet Khan, Rahim
Mohamad Ghazali, Farinazleen
Mahyudin, Nor Ainy
Putra Samsudin, Nik Iskandar
author_sort Khan, Rahim
title Co-inoculation of aflatoxigenic and non-aflatoxigenic strains of aspergillus flavus to assess the efficacy of non-aflatoxigenic strains in growth inhibition and aflatoxin b1 reduction
title_short Co-inoculation of aflatoxigenic and non-aflatoxigenic strains of aspergillus flavus to assess the efficacy of non-aflatoxigenic strains in growth inhibition and aflatoxin b1 reduction
title_full Co-inoculation of aflatoxigenic and non-aflatoxigenic strains of aspergillus flavus to assess the efficacy of non-aflatoxigenic strains in growth inhibition and aflatoxin b1 reduction
title_fullStr Co-inoculation of aflatoxigenic and non-aflatoxigenic strains of aspergillus flavus to assess the efficacy of non-aflatoxigenic strains in growth inhibition and aflatoxin b1 reduction
title_full_unstemmed Co-inoculation of aflatoxigenic and non-aflatoxigenic strains of aspergillus flavus to assess the efficacy of non-aflatoxigenic strains in growth inhibition and aflatoxin b1 reduction
title_sort co-inoculation of aflatoxigenic and non-aflatoxigenic strains of aspergillus flavus to assess the efficacy of non-aflatoxigenic strains in growth inhibition and aflatoxin b1 reduction
publisher Multidisciplinary Digital Publishing Institute
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/96428/
https://www.mdpi.com/2077-0472/11/3/198
_version_ 1758579862246785024
score 13.211869

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