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Biochemical composition, morphology and antimicrobial susceptibility pattern of Corynebacterium pseudotuberculosis biofilm

Caseous lymphadenitis (CLA) is a ruminant disease caused by Corynebacterium pseudotuberculosis, a Gram-positive facultative intracellular pathogen. The present work was performed to investigate the biochemical composition, morphology and antimicrobial susceptibility pattern of C. pseudotuberculosis...

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Main Authors: Yaacob, Mohamad Fakhri, Murata, Aika, Mohamad Nor, Nurul Hidayah, Abdullah, Faez Firdaus Jesse, Raja Yahya, Mohd Fakharul Zaman
Format: Article
Published: Elsevier 2021
Online Access:http://psasir.upm.edu.my/id/eprint/96218/
https://www.sciencedirect.com/science/article/pii/S1018364720303293
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spelling my.upm.eprints.962182023-01-31T03:07:21Z http://psasir.upm.edu.my/id/eprint/96218/ Biochemical composition, morphology and antimicrobial susceptibility pattern of Corynebacterium pseudotuberculosis biofilm Yaacob, Mohamad Fakhri Murata, Aika Mohamad Nor, Nurul Hidayah Abdullah, Faez Firdaus Jesse Raja Yahya, Mohd Fakharul Zaman Caseous lymphadenitis (CLA) is a ruminant disease caused by Corynebacterium pseudotuberculosis, a Gram-positive facultative intracellular pathogen. The present work was performed to investigate the biochemical composition, morphology and antimicrobial susceptibility pattern of C. pseudotuberculosis biofilm using Raman spectroscopy, field emission scanning electron microscopy (FESEM) and microplate biofilm assay respectively. Results showed that the 24-h-old biofilm was characterized by Raman spectral peaks at 615 cm−1 (CCC symmetric bend phenyl ring), 668 cm−1 (Valine) and 825 cm−1 (Ring breath Tyr.) whilst the 48-h-old and 72-h-old biofilms were characterized by Raman spectral peaks at 1400 cm−1 (COOsingle bond sym.), 1450 cm−1 (COOsingle bond sym.), 1581 cm−1 (Ring breath Trp.), 1650 cm−1 (COOsingle bond asym.) and 1725 cm−1 (Cdouble bondO str.). Raman spectra also revealed the biochemical heterogeneity in C. pseudotuberculosis biofilm. FESEM images clearly showed the biofilm cells which were surrounded by the extracellular matrix. Treatment with nalidixic acid, streptomycin, tetracyclin, ethylenediaminetetraacetic acid (EDTA) and dimethyl sulfoxide (DMSO) significantly (p < 0.05) inhibited the viability of C. pseudotuberculosis biofilm. The present study suggests that the biochemical composition of C. pseudotuberculosis biofilm may vary across different developmental stages. Meanwhile, nalidixic acid, streptomycin, tetracyclin, EDTA and DMSO may be useful in the treatment of CLA. Elsevier 2021 Article PeerReviewed Yaacob, Mohamad Fakhri and Murata, Aika and Mohamad Nor, Nurul Hidayah and Abdullah, Faez Firdaus Jesse and Raja Yahya, Mohd Fakharul Zaman (2021) Biochemical composition, morphology and antimicrobial susceptibility pattern of Corynebacterium pseudotuberculosis biofilm. Journal of King Saud University - Science, 33 (1). art. no. 101225. pp. 1-8. ISSN 1018-3647 https://www.sciencedirect.com/science/article/pii/S1018364720303293 10.1016/j.jksus.2020.10.022
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Caseous lymphadenitis (CLA) is a ruminant disease caused by Corynebacterium pseudotuberculosis, a Gram-positive facultative intracellular pathogen. The present work was performed to investigate the biochemical composition, morphology and antimicrobial susceptibility pattern of C. pseudotuberculosis biofilm using Raman spectroscopy, field emission scanning electron microscopy (FESEM) and microplate biofilm assay respectively. Results showed that the 24-h-old biofilm was characterized by Raman spectral peaks at 615 cm−1 (CCC symmetric bend phenyl ring), 668 cm−1 (Valine) and 825 cm−1 (Ring breath Tyr.) whilst the 48-h-old and 72-h-old biofilms were characterized by Raman spectral peaks at 1400 cm−1 (COOsingle bond sym.), 1450 cm−1 (COOsingle bond sym.), 1581 cm−1 (Ring breath Trp.), 1650 cm−1 (COOsingle bond asym.) and 1725 cm−1 (Cdouble bondO str.). Raman spectra also revealed the biochemical heterogeneity in C. pseudotuberculosis biofilm. FESEM images clearly showed the biofilm cells which were surrounded by the extracellular matrix. Treatment with nalidixic acid, streptomycin, tetracyclin, ethylenediaminetetraacetic acid (EDTA) and dimethyl sulfoxide (DMSO) significantly (p < 0.05) inhibited the viability of C. pseudotuberculosis biofilm. The present study suggests that the biochemical composition of C. pseudotuberculosis biofilm may vary across different developmental stages. Meanwhile, nalidixic acid, streptomycin, tetracyclin, EDTA and DMSO may be useful in the treatment of CLA.
format Article
author Yaacob, Mohamad Fakhri
Murata, Aika
Mohamad Nor, Nurul Hidayah
Abdullah, Faez Firdaus Jesse
Raja Yahya, Mohd Fakharul Zaman
spellingShingle Yaacob, Mohamad Fakhri
Murata, Aika
Mohamad Nor, Nurul Hidayah
Abdullah, Faez Firdaus Jesse
Raja Yahya, Mohd Fakharul Zaman
Biochemical composition, morphology and antimicrobial susceptibility pattern of Corynebacterium pseudotuberculosis biofilm
author_facet Yaacob, Mohamad Fakhri
Murata, Aika
Mohamad Nor, Nurul Hidayah
Abdullah, Faez Firdaus Jesse
Raja Yahya, Mohd Fakharul Zaman
author_sort Yaacob, Mohamad Fakhri
title Biochemical composition, morphology and antimicrobial susceptibility pattern of Corynebacterium pseudotuberculosis biofilm
title_short Biochemical composition, morphology and antimicrobial susceptibility pattern of Corynebacterium pseudotuberculosis biofilm
title_full Biochemical composition, morphology and antimicrobial susceptibility pattern of Corynebacterium pseudotuberculosis biofilm
title_fullStr Biochemical composition, morphology and antimicrobial susceptibility pattern of Corynebacterium pseudotuberculosis biofilm
title_full_unstemmed Biochemical composition, morphology and antimicrobial susceptibility pattern of Corynebacterium pseudotuberculosis biofilm
title_sort biochemical composition, morphology and antimicrobial susceptibility pattern of corynebacterium pseudotuberculosis biofilm
publisher Elsevier
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/96218/
https://www.sciencedirect.com/science/article/pii/S1018364720303293
_version_ 1756685784404983808
score 13.211869

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