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A synthetic curcuminoid analogue, 2,6-Bis-4-(Hydroxyl-3-Methoxybenzylidine)-Cyclohexanone (BHMC) ameliorates acute airway inflammation of allergic asthma in ovalbumin-sensitized mice

2,6-Bis-(4-hydroxyl-3-methoxybenzylidine) cyclohexanone (BHMC), a synthetic curcuminoid analogue, has been shown to exhibit anti-inflammatory properties in cellular models of inflammation and improve the survival of mice from lethal sepsis. We further evaluated the therapeutic effect of BHMC on acut...

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Main Authors: Tham, Chau Ling, Sin, Yee Yeoh, Ong, Chun Hao, Harith, Hanis Hazeera, Israf Ali, Daud Ahmad
Format: Article
Published: Hindawi Publishing Corporation 2021
Online Access:http://psasir.upm.edu.my/id/eprint/96134/
https://www.hindawi.com/journals/mi/2021/9725903/
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spelling my.upm.eprints.961342023-02-22T07:09:25Z http://psasir.upm.edu.my/id/eprint/96134/ A synthetic curcuminoid analogue, 2,6-Bis-4-(Hydroxyl-3-Methoxybenzylidine)-Cyclohexanone (BHMC) ameliorates acute airway inflammation of allergic asthma in ovalbumin-sensitized mice Tham, Chau Ling Sin, Yee Yeoh Ong, Chun Hao Harith, Hanis Hazeera Israf Ali, Daud Ahmad 2,6-Bis-(4-hydroxyl-3-methoxybenzylidine) cyclohexanone (BHMC), a synthetic curcuminoid analogue, has been shown to exhibit anti-inflammatory properties in cellular models of inflammation and improve the survival of mice from lethal sepsis. We further evaluated the therapeutic effect of BHMC on acute airway inflammation in a mouse model of allergic asthma. Mice were sensitized and challenged with ovalbumin (OVA), followed by intraperitoneal administration of 0.1, 1, and 10 mg/kg of BHMC. Bronchoalveolar lavage fluid, blood, and lung samples were collected, and the respiratory function was measured. OVA sensitization and challenge increased airway hyperresponsiveness (AHR) and pulmonary inflammation. All three doses of BHMC (0.1-10 mg/kg) significantly reduced the number of eosinophils, lymphocytes, macrophages, and neutrophils, as well as the levels of Th2 cytokines (IL-4, IL-5 and IL-13) in bronchoalveolar lavage fluid (BALF) as compared to OVA-challenged mice. However, serum level of IgE was not affected. All three doses of BHMC (0.1-10 mg/kg) were effective in suppressing the infiltration of inflammatory cells at the peribronchial and perivascular regions, with the greatest effect observed at 1 mg/kg which was comparable to dexamethasone. Goblet cell hyperplasia was inhibited by 1 and 10 mg/kg of BHMC, while the lowest dose (0.1 mg/kg) had no significant inhibitory effect. These findings demonstrate that BHMC, a synthetic nonsteroidal small molecule, ameliorates acute airway inflammation associated with allergic asthma, primarily by suppressing the release of inflammatory mediators and goblet cell hyperplasia to a lesser extent in acute airway inflammation of allergic asthma. Hindawi Publishing Corporation 2021 Article PeerReviewed Tham, Chau Ling and Sin, Yee Yeoh and Ong, Chun Hao and Harith, Hanis Hazeera and Israf Ali, Daud Ahmad (2021) A synthetic curcuminoid analogue, 2,6-Bis-4-(Hydroxyl-3-Methoxybenzylidine)-Cyclohexanone (BHMC) ameliorates acute airway inflammation of allergic asthma in ovalbumin-sensitized mice. Mediators of Inflammation, 2021. pp. 1-10. ISSN 0962-9351; ESSN: 1466-1861 https://www.hindawi.com/journals/mi/2021/9725903/ /10.1155/2021/9725903
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description 2,6-Bis-(4-hydroxyl-3-methoxybenzylidine) cyclohexanone (BHMC), a synthetic curcuminoid analogue, has been shown to exhibit anti-inflammatory properties in cellular models of inflammation and improve the survival of mice from lethal sepsis. We further evaluated the therapeutic effect of BHMC on acute airway inflammation in a mouse model of allergic asthma. Mice were sensitized and challenged with ovalbumin (OVA), followed by intraperitoneal administration of 0.1, 1, and 10 mg/kg of BHMC. Bronchoalveolar lavage fluid, blood, and lung samples were collected, and the respiratory function was measured. OVA sensitization and challenge increased airway hyperresponsiveness (AHR) and pulmonary inflammation. All three doses of BHMC (0.1-10 mg/kg) significantly reduced the number of eosinophils, lymphocytes, macrophages, and neutrophils, as well as the levels of Th2 cytokines (IL-4, IL-5 and IL-13) in bronchoalveolar lavage fluid (BALF) as compared to OVA-challenged mice. However, serum level of IgE was not affected. All three doses of BHMC (0.1-10 mg/kg) were effective in suppressing the infiltration of inflammatory cells at the peribronchial and perivascular regions, with the greatest effect observed at 1 mg/kg which was comparable to dexamethasone. Goblet cell hyperplasia was inhibited by 1 and 10 mg/kg of BHMC, while the lowest dose (0.1 mg/kg) had no significant inhibitory effect. These findings demonstrate that BHMC, a synthetic nonsteroidal small molecule, ameliorates acute airway inflammation associated with allergic asthma, primarily by suppressing the release of inflammatory mediators and goblet cell hyperplasia to a lesser extent in acute airway inflammation of allergic asthma.
format Article
author Tham, Chau Ling
Sin, Yee Yeoh
Ong, Chun Hao
Harith, Hanis Hazeera
Israf Ali, Daud Ahmad
spellingShingle Tham, Chau Ling
Sin, Yee Yeoh
Ong, Chun Hao
Harith, Hanis Hazeera
Israf Ali, Daud Ahmad
A synthetic curcuminoid analogue, 2,6-Bis-4-(Hydroxyl-3-Methoxybenzylidine)-Cyclohexanone (BHMC) ameliorates acute airway inflammation of allergic asthma in ovalbumin-sensitized mice
author_facet Tham, Chau Ling
Sin, Yee Yeoh
Ong, Chun Hao
Harith, Hanis Hazeera
Israf Ali, Daud Ahmad
author_sort Tham, Chau Ling
title A synthetic curcuminoid analogue, 2,6-Bis-4-(Hydroxyl-3-Methoxybenzylidine)-Cyclohexanone (BHMC) ameliorates acute airway inflammation of allergic asthma in ovalbumin-sensitized mice
title_short A synthetic curcuminoid analogue, 2,6-Bis-4-(Hydroxyl-3-Methoxybenzylidine)-Cyclohexanone (BHMC) ameliorates acute airway inflammation of allergic asthma in ovalbumin-sensitized mice
title_full A synthetic curcuminoid analogue, 2,6-Bis-4-(Hydroxyl-3-Methoxybenzylidine)-Cyclohexanone (BHMC) ameliorates acute airway inflammation of allergic asthma in ovalbumin-sensitized mice
title_fullStr A synthetic curcuminoid analogue, 2,6-Bis-4-(Hydroxyl-3-Methoxybenzylidine)-Cyclohexanone (BHMC) ameliorates acute airway inflammation of allergic asthma in ovalbumin-sensitized mice
title_full_unstemmed A synthetic curcuminoid analogue, 2,6-Bis-4-(Hydroxyl-3-Methoxybenzylidine)-Cyclohexanone (BHMC) ameliorates acute airway inflammation of allergic asthma in ovalbumin-sensitized mice
title_sort synthetic curcuminoid analogue, 2,6-bis-4-(hydroxyl-3-methoxybenzylidine)-cyclohexanone (bhmc) ameliorates acute airway inflammation of allergic asthma in ovalbumin-sensitized mice
publisher Hindawi Publishing Corporation
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/96134/
https://www.hindawi.com/journals/mi/2021/9725903/
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score 13.211869

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