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Asp-376-Glu substitution endows target-site resistance to AHAS inhibitors in Limnocharis flava, an invasive weed in tropical rice fields

Limnocharis flava (L.) Buchenau is a problematic weed in rice fields and water canals of Southeast Asia, and in Malaysia this invasive aquatic weed species has evolved multiple resistance to synthetic auxin herbicide and acetohydroxyacid synthase (AHAS) inhibitors. In this study, it was revealed tha...

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Main Authors: Zakaria, Norazua, Ruzmi, Rabiatuladawiyah, Moosa, Salmah, Asib, Norhayu, Zulperi, Dzarifah, Ismail, Siti Izera, Ahmad Hamdani, Muhammad Saiful
Format: Article
Published: Springer 2021
Online Access:http://psasir.upm.edu.my/id/eprint/96056/
https://link.springer.com/article/10.1007/s12298-021-00987-3
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spelling my.upm.eprints.960562023-03-09T02:43:18Z http://psasir.upm.edu.my/id/eprint/96056/ Asp-376-Glu substitution endows target-site resistance to AHAS inhibitors in Limnocharis flava, an invasive weed in tropical rice fields Zakaria, Norazua Ruzmi, Rabiatuladawiyah Moosa, Salmah Asib, Norhayu Zulperi, Dzarifah Ismail, Siti Izera Ahmad Hamdani, Muhammad Saiful Limnocharis flava (L.) Buchenau is a problematic weed in rice fields and water canals of Southeast Asia, and in Malaysia this invasive aquatic weed species has evolved multiple resistance to synthetic auxin herbicide and acetohydroxyacid synthase (AHAS) inhibitors. In this study, it was revealed that, a single nucleotide polymorphism (SNP) at amino acid position 376, where C was substituted to G at the third base of the same codon (GAC to GAG), resulting in Aspartate (Asp) substitution by Glutamate (Glu) was the contributing resistance mechanism in the L. flava population to AHAS inhibitors. In vitro assay further proved that, all the L. flava individuals carrying AHAS resistance mutation exhibited decreased-sensitivity to AHAS inhibitors at the enzyme level. In the bensulfuron-methyl whole-plant bioassay, high resistance indices (RI) of 328- and 437-fold were recorded in the absence and presence of malathion (the P450 inhibitor), respectively. Similarly, translocation and absorption of bensulfuron-methyl in both resistant and susceptible L. flava populations showed no remarkable differences, hence eliminated the possible co-existence of non-target-site resistance mechanism in the resistant L. flava. This study has confirmed another new case of a target-site resistant weed species to AHAS-inhibitors. Springer 2021 Article PeerReviewed Zakaria, Norazua and Ruzmi, Rabiatuladawiyah and Moosa, Salmah and Asib, Norhayu and Zulperi, Dzarifah and Ismail, Siti Izera and Ahmad Hamdani, Muhammad Saiful (2021) Asp-376-Glu substitution endows target-site resistance to AHAS inhibitors in Limnocharis flava, an invasive weed in tropical rice fields. Physiology and Molecular Biology of Plants, 274 (5). pp. 969-983. ISSN 0971-5894; ESSN: 0974-0430 https://link.springer.com/article/10.1007/s12298-021-00987-3 10.1007/s12298-021-00987-3
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Limnocharis flava (L.) Buchenau is a problematic weed in rice fields and water canals of Southeast Asia, and in Malaysia this invasive aquatic weed species has evolved multiple resistance to synthetic auxin herbicide and acetohydroxyacid synthase (AHAS) inhibitors. In this study, it was revealed that, a single nucleotide polymorphism (SNP) at amino acid position 376, where C was substituted to G at the third base of the same codon (GAC to GAG), resulting in Aspartate (Asp) substitution by Glutamate (Glu) was the contributing resistance mechanism in the L. flava population to AHAS inhibitors. In vitro assay further proved that, all the L. flava individuals carrying AHAS resistance mutation exhibited decreased-sensitivity to AHAS inhibitors at the enzyme level. In the bensulfuron-methyl whole-plant bioassay, high resistance indices (RI) of 328- and 437-fold were recorded in the absence and presence of malathion (the P450 inhibitor), respectively. Similarly, translocation and absorption of bensulfuron-methyl in both resistant and susceptible L. flava populations showed no remarkable differences, hence eliminated the possible co-existence of non-target-site resistance mechanism in the resistant L. flava. This study has confirmed another new case of a target-site resistant weed species to AHAS-inhibitors.
format Article
author Zakaria, Norazua
Ruzmi, Rabiatuladawiyah
Moosa, Salmah
Asib, Norhayu
Zulperi, Dzarifah
Ismail, Siti Izera
Ahmad Hamdani, Muhammad Saiful
spellingShingle Zakaria, Norazua
Ruzmi, Rabiatuladawiyah
Moosa, Salmah
Asib, Norhayu
Zulperi, Dzarifah
Ismail, Siti Izera
Ahmad Hamdani, Muhammad Saiful
Asp-376-Glu substitution endows target-site resistance to AHAS inhibitors in Limnocharis flava, an invasive weed in tropical rice fields
author_facet Zakaria, Norazua
Ruzmi, Rabiatuladawiyah
Moosa, Salmah
Asib, Norhayu
Zulperi, Dzarifah
Ismail, Siti Izera
Ahmad Hamdani, Muhammad Saiful
author_sort Zakaria, Norazua
title Asp-376-Glu substitution endows target-site resistance to AHAS inhibitors in Limnocharis flava, an invasive weed in tropical rice fields
title_short Asp-376-Glu substitution endows target-site resistance to AHAS inhibitors in Limnocharis flava, an invasive weed in tropical rice fields
title_full Asp-376-Glu substitution endows target-site resistance to AHAS inhibitors in Limnocharis flava, an invasive weed in tropical rice fields
title_fullStr Asp-376-Glu substitution endows target-site resistance to AHAS inhibitors in Limnocharis flava, an invasive weed in tropical rice fields
title_full_unstemmed Asp-376-Glu substitution endows target-site resistance to AHAS inhibitors in Limnocharis flava, an invasive weed in tropical rice fields
title_sort asp-376-glu substitution endows target-site resistance to ahas inhibitors in limnocharis flava, an invasive weed in tropical rice fields
publisher Springer
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/96056/
https://link.springer.com/article/10.1007/s12298-021-00987-3
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score 13.211869

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