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A recommendation for a pre-standardized marine microalgal dry 3 weight determination protocol for laboratory scale culture using 4 ammonium formate as a washing agent

Microalgal biomass is one of the crucial criteria in microalgal studies. Many reported methods, even the well-established protocol on microalgal dry weight (DW) determination, vary greatly, and reliable comparative assessment amongst published results could be problematic. This study aimed to determ...

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Main Authors: Khaw, Yam Sim, Tan, Hui Teng, Sopawong, Arissara, Shaharuddin, Noor Azmi, Omar, Abdul Rahman, Md. Yusoff, Fatimah
Format: Article
Published: Multidisciplinary Digital Publishing Institute 2021
Online Access:http://psasir.upm.edu.my/id/eprint/95997/
https://www.mdpi.com/2079-7737/10/8/799
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spelling my.upm.eprints.959972023-03-09T03:48:20Z http://psasir.upm.edu.my/id/eprint/95997/ A recommendation for a pre-standardized marine microalgal dry 3 weight determination protocol for laboratory scale culture using 4 ammonium formate as a washing agent Khaw, Yam Sim Tan, Hui Teng Sopawong, Arissara Shaharuddin, Noor Azmi Omar, Abdul Rahman Md. Yusoff, Fatimah Microalgal biomass is one of the crucial criteria in microalgal studies. Many reported methods, even the well-established protocol on microalgal dry weight (DW) determination, vary greatly, and reliable comparative assessment amongst published results could be problematic. This study aimed to determine the best condition of critical parameters in marine microalgal DW determination for laboratory-scale culture using four different marine microalgal species. These parameters included the washing process, grades of glass microfiber filter (GMF), GMF pretreatment conditions, washing agent (ammonium formate) concentrations, culture: washing agent ratios (v:v) and washing cycles. GMF grade GF/A with precombustion at 450 °C provided the most satisfactory DW and the highest ash-free dry weight (AFDW)/DW ratio. Furthermore, 0.05 M ammonium formate with 1:2 culture: washing agent ratio and a minimum of two washing cycles appeared to be the best settings of microalgal DW determination. The present treatment increased the AFDW/DW ratio of the four respective microalgae by a minimum of 19%. The findings of this study could serve as a pivotal reference in developing a standardized protocol of marine microalgal DW determination to obtain veracious and reliable marine microalgal DW. Multidisciplinary Digital Publishing Institute 2021 Article PeerReviewed Khaw, Yam Sim and Tan, Hui Teng and Sopawong, Arissara and Shaharuddin, Noor Azmi and Omar, Abdul Rahman and Md. Yusoff, Fatimah (2021) A recommendation for a pre-standardized marine microalgal dry 3 weight determination protocol for laboratory scale culture using 4 ammonium formate as a washing agent. Biology-Basel, 10 (8). art. no. 799. pp. 1-22. ISSN 2079-7737 https://www.mdpi.com/2079-7737/10/8/799 10.3390/biology10080799
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Microalgal biomass is one of the crucial criteria in microalgal studies. Many reported methods, even the well-established protocol on microalgal dry weight (DW) determination, vary greatly, and reliable comparative assessment amongst published results could be problematic. This study aimed to determine the best condition of critical parameters in marine microalgal DW determination for laboratory-scale culture using four different marine microalgal species. These parameters included the washing process, grades of glass microfiber filter (GMF), GMF pretreatment conditions, washing agent (ammonium formate) concentrations, culture: washing agent ratios (v:v) and washing cycles. GMF grade GF/A with precombustion at 450 °C provided the most satisfactory DW and the highest ash-free dry weight (AFDW)/DW ratio. Furthermore, 0.05 M ammonium formate with 1:2 culture: washing agent ratio and a minimum of two washing cycles appeared to be the best settings of microalgal DW determination. The present treatment increased the AFDW/DW ratio of the four respective microalgae by a minimum of 19%. The findings of this study could serve as a pivotal reference in developing a standardized protocol of marine microalgal DW determination to obtain veracious and reliable marine microalgal DW.
format Article
author Khaw, Yam Sim
Tan, Hui Teng
Sopawong, Arissara
Shaharuddin, Noor Azmi
Omar, Abdul Rahman
Md. Yusoff, Fatimah
spellingShingle Khaw, Yam Sim
Tan, Hui Teng
Sopawong, Arissara
Shaharuddin, Noor Azmi
Omar, Abdul Rahman
Md. Yusoff, Fatimah
A recommendation for a pre-standardized marine microalgal dry 3 weight determination protocol for laboratory scale culture using 4 ammonium formate as a washing agent
author_facet Khaw, Yam Sim
Tan, Hui Teng
Sopawong, Arissara
Shaharuddin, Noor Azmi
Omar, Abdul Rahman
Md. Yusoff, Fatimah
author_sort Khaw, Yam Sim
title A recommendation for a pre-standardized marine microalgal dry 3 weight determination protocol for laboratory scale culture using 4 ammonium formate as a washing agent
title_short A recommendation for a pre-standardized marine microalgal dry 3 weight determination protocol for laboratory scale culture using 4 ammonium formate as a washing agent
title_full A recommendation for a pre-standardized marine microalgal dry 3 weight determination protocol for laboratory scale culture using 4 ammonium formate as a washing agent
title_fullStr A recommendation for a pre-standardized marine microalgal dry 3 weight determination protocol for laboratory scale culture using 4 ammonium formate as a washing agent
title_full_unstemmed A recommendation for a pre-standardized marine microalgal dry 3 weight determination protocol for laboratory scale culture using 4 ammonium formate as a washing agent
title_sort recommendation for a pre-standardized marine microalgal dry 3 weight determination protocol for laboratory scale culture using 4 ammonium formate as a washing agent
publisher Multidisciplinary Digital Publishing Institute
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/95997/
https://www.mdpi.com/2079-7737/10/8/799
_version_ 1761620375103340544
score 13.211869

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