Staff View: Antibiofilm activity of Oxytetracycline loaded calcium carbonate aragonite loaded nanoparticle against Corynebacterium Pseudotuberculosis

Antibiofilm activity of Oxytetracycline loaded calcium carbonate aragonite loaded nanoparticle against Corynebacterium Pseudotuberculosis

Background and Objective: Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis, a zoonotic bacterial disease of small ruminants. The disease is unresponsive to antibiotic treatment due to the intracellular nature of the bacteria, thick purulent exudates, antibiotic resi...

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Main Authors:	Banke, Idris Sherifat, Abdul Kadir, Arifah, Abdullah Jesse, Faez Firdaus, Ramanoon, Siti Zubaidah, Muhammad, Abdul Basit, Zakaria, Md Zuki Abu Bakar
Format:	Article
Published:	Asian Network for Scientific Information (ANSINET) 2021
Online Access:	http://psasir.upm.edu.my/id/eprint/95897/ https://scialert.net/abstract/?doi=ijp.2021.73.83
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spelling	my.upm.eprints.958972023-01-26T01:56:34Z http://psasir.upm.edu.my/id/eprint/95897/ Antibiofilm activity of Oxytetracycline loaded calcium carbonate aragonite loaded nanoparticle against Corynebacterium Pseudotuberculosis Banke, Idris Sherifat Abdul Kadir, Arifah Abdullah Jesse, Faez Firdaus Ramanoon, Siti Zubaidah Muhammad, Abdul Basit Zakaria, Md Zuki Abu Bakar Background and Objective: Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis, a zoonotic bacterial disease of small ruminants. The disease is unresponsive to antibiotic treatment due to the intracellular nature of the bacteria, thick purulent exudates, antibiotic resistance and formation of biofilms. The objective of this study was to evaluate the antibiofilm activity of oxytetracycline in free (OTC) and cockle shell loaded forms (OTC-CS-CaCO3NP) against C. pseudotuberculosis. Materials and Methods: The Minimum Inhibitory Concentration (MIC) and Minimum Biofilm Eradication Concentration (MBEC) of oxytetracycline in free and cockle shell loaded forms against C. pseudotuberculosis were determined by the microtiter plate assay method. Biofilm formation and inhibition assays were studied spectrophotometrically using the crystal violet assay method. TEM, SEM and fluorescent microscopy were used to assess the antibacterial and antibiofilm effect against C. pseudotuberculosis. Results: The MIC of OTC and OTC-CS-CaCO3NP against C. pseudotuberculosis were 125 and 500 μg L–1, respectively while the MBEC was 250 and >2000 μg L–1. TEM analysis showed OTC-CS-CaCO3NP disrupted cell membrane and formation of vacuoles in C. pseudotuberculosis. Conclusion: Microscopic analysis of the biofilms revealed OTC-CS-CaCO3NP caused significant morphological changes compared to OTC. These results suggest the potential applicability of OTC-CS-CaCO3NP for the treatment of caseous lymphadenitis. Asian Network for Scientific Information (ANSINET) 2021 Article PeerReviewed Banke, Idris Sherifat and Abdul Kadir, Arifah and Abdullah Jesse, Faez Firdaus and Ramanoon, Siti Zubaidah and Muhammad, Abdul Basit and Zakaria, Md Zuki Abu Bakar (2021) Antibiofilm activity of Oxytetracycline loaded calcium carbonate aragonite loaded nanoparticle against Corynebacterium Pseudotuberculosis. International Journal of Pharmacology, 17 (2). 73 - 83. ISSN 1811-7775; ESSN: 1812-5700 https://scialert.net/abstract/?doi=ijp.2021.73.83 10.3923/ijp.2021.73.83
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	Background and Objective: Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis, a zoonotic bacterial disease of small ruminants. The disease is unresponsive to antibiotic treatment due to the intracellular nature of the bacteria, thick purulent exudates, antibiotic resistance and formation of biofilms. The objective of this study was to evaluate the antibiofilm activity of oxytetracycline in free (OTC) and cockle shell loaded forms (OTC-CS-CaCO3NP) against C. pseudotuberculosis. Materials and Methods: The Minimum Inhibitory Concentration (MIC) and Minimum Biofilm Eradication Concentration (MBEC) of oxytetracycline in free and cockle shell loaded forms against C. pseudotuberculosis were determined by the microtiter plate assay method. Biofilm formation and inhibition assays were studied spectrophotometrically using the crystal violet assay method. TEM, SEM and fluorescent microscopy were used to assess the antibacterial and antibiofilm effect against C. pseudotuberculosis. Results: The MIC of OTC and OTC-CS-CaCO3NP against C. pseudotuberculosis were 125 and 500 μg L–1, respectively while the MBEC was 250 and >2000 μg L–1. TEM analysis showed OTC-CS-CaCO3NP disrupted cell membrane and formation of vacuoles in C. pseudotuberculosis. Conclusion: Microscopic analysis of the biofilms revealed OTC-CS-CaCO3NP caused significant morphological changes compared to OTC. These results suggest the potential applicability of OTC-CS-CaCO3NP for the treatment of caseous lymphadenitis.
format	Article
author	Banke, Idris Sherifat Abdul Kadir, Arifah Abdullah Jesse, Faez Firdaus Ramanoon, Siti Zubaidah Muhammad, Abdul Basit Zakaria, Md Zuki Abu Bakar
spellingShingle	Banke, Idris Sherifat Abdul Kadir, Arifah Abdullah Jesse, Faez Firdaus Ramanoon, Siti Zubaidah Muhammad, Abdul Basit Zakaria, Md Zuki Abu Bakar Antibiofilm activity of Oxytetracycline loaded calcium carbonate aragonite loaded nanoparticle against Corynebacterium Pseudotuberculosis
author_facet	Banke, Idris Sherifat Abdul Kadir, Arifah Abdullah Jesse, Faez Firdaus Ramanoon, Siti Zubaidah Muhammad, Abdul Basit Zakaria, Md Zuki Abu Bakar
author_sort	Banke, Idris Sherifat
title	Antibiofilm activity of Oxytetracycline loaded calcium carbonate aragonite loaded nanoparticle against Corynebacterium Pseudotuberculosis
title_short	Antibiofilm activity of Oxytetracycline loaded calcium carbonate aragonite loaded nanoparticle against Corynebacterium Pseudotuberculosis
title_full	Antibiofilm activity of Oxytetracycline loaded calcium carbonate aragonite loaded nanoparticle against Corynebacterium Pseudotuberculosis
title_fullStr	Antibiofilm activity of Oxytetracycline loaded calcium carbonate aragonite loaded nanoparticle against Corynebacterium Pseudotuberculosis
title_full_unstemmed	Antibiofilm activity of Oxytetracycline loaded calcium carbonate aragonite loaded nanoparticle against Corynebacterium Pseudotuberculosis
title_sort	antibiofilm activity of oxytetracycline loaded calcium carbonate aragonite loaded nanoparticle against corynebacterium pseudotuberculosis
publisher	Asian Network for Scientific Information (ANSINET)
publishDate	2021
url	http://psasir.upm.edu.my/id/eprint/95897/ https://scialert.net/abstract/?doi=ijp.2021.73.83
_version_	1756061499358445568
score	13.211869

Antibiofilm activity of Oxytetracycline loaded calcium carbonate aragonite loaded nanoparticle against Corynebacterium Pseudotuberculosis

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