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Antibacterial potential of biosynthesized zinc oxide nanoparticles against poultry-associated foodborne pathogens: an in vitro study

Since the emergence of multidrug-resistant bacteria in the poultry industry is currently a serious threat, there is an urgent need to develop a more efficient and alternative antibacterial substance. Zinc oxide nanoparticles (ZnO NPs) have exhibited antibacterial efficacy against a wide range of mic...

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Main Authors: Mohd Yusof, Hidayat, Abdul Rahman, Nor’Aini, Mohamad, Rosfarizan, Zaidan, Uswatun Hasanah, Samsudin, Anjas Asmara @ Ab Hadi
Format: Article
Published: Multidisciplinary Digital Publishing Institute 2021
Online Access:http://psasir.upm.edu.my/id/eprint/95894/
https://www.mdpi.com/2076-2615/11/7/2093
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spelling my.upm.eprints.958942023-01-26T01:56:00Z http://psasir.upm.edu.my/id/eprint/95894/ Antibacterial potential of biosynthesized zinc oxide nanoparticles against poultry-associated foodborne pathogens: an in vitro study Mohd Yusof, Hidayat Abdul Rahman, Nor’Aini Mohamad, Rosfarizan Zaidan, Uswatun Hasanah Samsudin, Anjas Asmara @ Ab Hadi Since the emergence of multidrug-resistant bacteria in the poultry industry is currently a serious threat, there is an urgent need to develop a more efficient and alternative antibacterial substance. Zinc oxide nanoparticles (ZnO NPs) have exhibited antibacterial efficacy against a wide range of microorganisms. Although the in vitro antibacterial activity of ZnO NPs has been studied, little is known about the antibacterial mechanisms of ZnO NPs against poultry-associated foodborne pathogens. In the present study, ZnO NPs were successfully synthesized using Lactobacillus plantarum TA4, characterized, and their antibacterial potential against common avian pathogens (Salmonella spp., Escherichia coli, and Staphylococcus aureus) was investigated. Confirmation of ZnO NPs by UV-Visual spectroscopy showed an absorption band center at 360 nm. Morphologically, the synthesized ZnO NPs were oval with an average particle size of 29.7 nm. Based on the dissolution study of Zn2+, ZnO NPs released more ions than their bulk counterparts. Results from the agar well diffusion assay indicated that ZnO NPs effectively inhibited the growth of the three poultry-associated foodborne pathogens. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were assessed using various concentrations of ZnO NPs, which resulted in excellent antibacterial activity as compared to their bulkier counterparts. S. aureus was more susceptible to ZnO NPs compared to the other tested bacteria. Furthermore, the ZnO NPs demonstrated substantial biofilm inhibition and eradication. The formation of reactive oxygen species (ROS) and cellular material leakage was quantified to determine the underlying antibacterial mechanisms, whereas a scanning electron microscope (SEM) was used to examine the morphological changes of tested bacteria treated with ZnO NPs. The findings suggested that ROS-induced oxidative stress caused membrane damage and bacterial cell death. Overall, the results demonstrated that ZnO NPs could be developed as an alternative antibiotic in poultry production and revealed new possibilities in combating pathogenic microorganisms. Multidisciplinary Digital Publishing Institute 2021 Article PeerReviewed Mohd Yusof, Hidayat and Abdul Rahman, Nor’Aini and Mohamad, Rosfarizan and Zaidan, Uswatun Hasanah and Samsudin, Anjas Asmara @ Ab Hadi (2021) Antibacterial potential of biosynthesized zinc oxide nanoparticles against poultry-associated foodborne pathogens: an in vitro study. Animals, 11 (7). art. no. 2093. pp. 1-26. ISSN 2076-2615 https://www.mdpi.com/2076-2615/11/7/2093 10.3390/ani11072093
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Since the emergence of multidrug-resistant bacteria in the poultry industry is currently a serious threat, there is an urgent need to develop a more efficient and alternative antibacterial substance. Zinc oxide nanoparticles (ZnO NPs) have exhibited antibacterial efficacy against a wide range of microorganisms. Although the in vitro antibacterial activity of ZnO NPs has been studied, little is known about the antibacterial mechanisms of ZnO NPs against poultry-associated foodborne pathogens. In the present study, ZnO NPs were successfully synthesized using Lactobacillus plantarum TA4, characterized, and their antibacterial potential against common avian pathogens (Salmonella spp., Escherichia coli, and Staphylococcus aureus) was investigated. Confirmation of ZnO NPs by UV-Visual spectroscopy showed an absorption band center at 360 nm. Morphologically, the synthesized ZnO NPs were oval with an average particle size of 29.7 nm. Based on the dissolution study of Zn2+, ZnO NPs released more ions than their bulk counterparts. Results from the agar well diffusion assay indicated that ZnO NPs effectively inhibited the growth of the three poultry-associated foodborne pathogens. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were assessed using various concentrations of ZnO NPs, which resulted in excellent antibacterial activity as compared to their bulkier counterparts. S. aureus was more susceptible to ZnO NPs compared to the other tested bacteria. Furthermore, the ZnO NPs demonstrated substantial biofilm inhibition and eradication. The formation of reactive oxygen species (ROS) and cellular material leakage was quantified to determine the underlying antibacterial mechanisms, whereas a scanning electron microscope (SEM) was used to examine the morphological changes of tested bacteria treated with ZnO NPs. The findings suggested that ROS-induced oxidative stress caused membrane damage and bacterial cell death. Overall, the results demonstrated that ZnO NPs could be developed as an alternative antibiotic in poultry production and revealed new possibilities in combating pathogenic microorganisms.
format Article
author Mohd Yusof, Hidayat
Abdul Rahman, Nor’Aini
Mohamad, Rosfarizan
Zaidan, Uswatun Hasanah
Samsudin, Anjas Asmara @ Ab Hadi
spellingShingle Mohd Yusof, Hidayat
Abdul Rahman, Nor’Aini
Mohamad, Rosfarizan
Zaidan, Uswatun Hasanah
Samsudin, Anjas Asmara @ Ab Hadi
Antibacterial potential of biosynthesized zinc oxide nanoparticles against poultry-associated foodborne pathogens: an in vitro study
author_facet Mohd Yusof, Hidayat
Abdul Rahman, Nor’Aini
Mohamad, Rosfarizan
Zaidan, Uswatun Hasanah
Samsudin, Anjas Asmara @ Ab Hadi
author_sort Mohd Yusof, Hidayat
title Antibacterial potential of biosynthesized zinc oxide nanoparticles against poultry-associated foodborne pathogens: an in vitro study
title_short Antibacterial potential of biosynthesized zinc oxide nanoparticles against poultry-associated foodborne pathogens: an in vitro study
title_full Antibacterial potential of biosynthesized zinc oxide nanoparticles against poultry-associated foodborne pathogens: an in vitro study
title_fullStr Antibacterial potential of biosynthesized zinc oxide nanoparticles against poultry-associated foodborne pathogens: an in vitro study
title_full_unstemmed Antibacterial potential of biosynthesized zinc oxide nanoparticles against poultry-associated foodborne pathogens: an in vitro study
title_sort antibacterial potential of biosynthesized zinc oxide nanoparticles against poultry-associated foodborne pathogens: an in vitro study
publisher Multidisciplinary Digital Publishing Institute
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/95894/
https://www.mdpi.com/2076-2615/11/7/2093
_version_ 1756061499062747136
score 13.211869

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