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The potential use of sialic acid from edible bird’s nest to attenuate mitochondrial dysfunction by in vitro study

Edible bird’s nest (EBN) is one of the expensive functional foods in herbal medicine. One of the major glyconutrients in EBN is sialic acid, which has a beneficial effect on neurological and intellectual capability in mammals. The aims of this research were to study the effects of sialic acid from E...

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Main Authors: Abd Rashed, Aswir, Ahmad, Hafandi, Abdul Khalid, Siti Khadijah, Rathi, Devi-Nair Gunasegavan
Format: Article
Language:English
Published: Frontiers Research Foundation 2021
Online Access:http://psasir.upm.edu.my/id/eprint/95564/1/The%20potential%20use%20of%20sialic%20acid%20from%20edible%20bird%E2%80%99s%20nest%20to%20attenuate%20mitochondrial%20dysfunction%20by%20in%20vitro%20study.pdf
http://psasir.upm.edu.my/id/eprint/95564/
https://www.frontiersin.org/articles/10.3389/fphar.2021.633303/full
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spelling my.upm.eprints.955642022-08-02T07:13:13Z http://psasir.upm.edu.my/id/eprint/95564/ The potential use of sialic acid from edible bird’s nest to attenuate mitochondrial dysfunction by in vitro study Abd Rashed, Aswir Ahmad, Hafandi Abdul Khalid, Siti Khadijah Rathi, Devi-Nair Gunasegavan Edible bird’s nest (EBN) is one of the expensive functional foods in herbal medicine. One of the major glyconutrients in EBN is sialic acid, which has a beneficial effect on neurological and intellectual capability in mammals. The aims of this research were to study the effects of sialic acid from EBN on cell viability and to determine its effect on mitochondria membrane potential (MtMP) in Caco-2, SK-N-MC, SH-SY5Y, and PC-12 cell lines. Fourteen samples of raw EBN were collected from four different states in Malaysia. The confluency of the epithelial monolayers measurement of the tight junction for all the cell lines was determined using transepithelial electrical resistance (TEER), and the sialic acid uptake study in cell lines was determined by using ultra-high performance liquid chromatography (UHPLC). The MTT assay was conducted for cell viability study. The MtMP in cell lines was determined using the Mito Probe JC-1 Assay by flow cytometer analysis. We have recorded a statistically significant difference between the uptake of sialic acid from EBN and the standard solution. A higher amount of sialic acid was absorbed by the cells from extract of EBN compared to the standard solution. The amounts of sialic acid uptake in Caco-2, SK-N-MC, SH-SY5Y, and PC-12 cell lines were (0.019 ± 0.001), (0.034 ± 0.006), (0.021 ± 0.002), and (0.025 ± 0.000) µmol/L, respectively. The MTT results indicated that the concentration of sialic acid increased the cell viability and showed no cytotoxicity effects on cell lines when they were exposed to the sialic acid extract and sialic acid standard at all the tested concentrations. The number of active mitochondria was found to be significantly higher in SH-SY5Y cell lines with a 195% increase when treated with sialic acid from EBN. Although many researchers around the globe use SHSY5Y and SK-N-MC for Alzheimer’s disease (AD) study, based on our finding, SH-SY5Y was found to be the most suitable cell line for AD study by in vitro works where it has a known relationship with mitochondrial dysfunction. Frontiers Research Foundation 2021-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/95564/1/The%20potential%20use%20of%20sialic%20acid%20from%20edible%20bird%E2%80%99s%20nest%20to%20attenuate%20mitochondrial%20dysfunction%20by%20in%20vitro%20study.pdf Abd Rashed, Aswir and Ahmad, Hafandi and Abdul Khalid, Siti Khadijah and Rathi, Devi-Nair Gunasegavan (2021) The potential use of sialic acid from edible bird’s nest to attenuate mitochondrial dysfunction by in vitro study. Frontiers in Pharmacology, 12. art. no. 633303. pp. 1-12. ISSN 1663-9812 https://www.frontiersin.org/articles/10.3389/fphar.2021.633303/full 10.3389/fphar.2021.633303
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Edible bird’s nest (EBN) is one of the expensive functional foods in herbal medicine. One of the major glyconutrients in EBN is sialic acid, which has a beneficial effect on neurological and intellectual capability in mammals. The aims of this research were to study the effects of sialic acid from EBN on cell viability and to determine its effect on mitochondria membrane potential (MtMP) in Caco-2, SK-N-MC, SH-SY5Y, and PC-12 cell lines. Fourteen samples of raw EBN were collected from four different states in Malaysia. The confluency of the epithelial monolayers measurement of the tight junction for all the cell lines was determined using transepithelial electrical resistance (TEER), and the sialic acid uptake study in cell lines was determined by using ultra-high performance liquid chromatography (UHPLC). The MTT assay was conducted for cell viability study. The MtMP in cell lines was determined using the Mito Probe JC-1 Assay by flow cytometer analysis. We have recorded a statistically significant difference between the uptake of sialic acid from EBN and the standard solution. A higher amount of sialic acid was absorbed by the cells from extract of EBN compared to the standard solution. The amounts of sialic acid uptake in Caco-2, SK-N-MC, SH-SY5Y, and PC-12 cell lines were (0.019 ± 0.001), (0.034 ± 0.006), (0.021 ± 0.002), and (0.025 ± 0.000) µmol/L, respectively. The MTT results indicated that the concentration of sialic acid increased the cell viability and showed no cytotoxicity effects on cell lines when they were exposed to the sialic acid extract and sialic acid standard at all the tested concentrations. The number of active mitochondria was found to be significantly higher in SH-SY5Y cell lines with a 195% increase when treated with sialic acid from EBN. Although many researchers around the globe use SHSY5Y and SK-N-MC for Alzheimer’s disease (AD) study, based on our finding, SH-SY5Y was found to be the most suitable cell line for AD study by in vitro works where it has a known relationship with mitochondrial dysfunction.
format Article
author Abd Rashed, Aswir
Ahmad, Hafandi
Abdul Khalid, Siti Khadijah
Rathi, Devi-Nair Gunasegavan
spellingShingle Abd Rashed, Aswir
Ahmad, Hafandi
Abdul Khalid, Siti Khadijah
Rathi, Devi-Nair Gunasegavan
The potential use of sialic acid from edible bird’s nest to attenuate mitochondrial dysfunction by in vitro study
author_facet Abd Rashed, Aswir
Ahmad, Hafandi
Abdul Khalid, Siti Khadijah
Rathi, Devi-Nair Gunasegavan
author_sort Abd Rashed, Aswir
title The potential use of sialic acid from edible bird’s nest to attenuate mitochondrial dysfunction by in vitro study
title_short The potential use of sialic acid from edible bird’s nest to attenuate mitochondrial dysfunction by in vitro study
title_full The potential use of sialic acid from edible bird’s nest to attenuate mitochondrial dysfunction by in vitro study
title_fullStr The potential use of sialic acid from edible bird’s nest to attenuate mitochondrial dysfunction by in vitro study
title_full_unstemmed The potential use of sialic acid from edible bird’s nest to attenuate mitochondrial dysfunction by in vitro study
title_sort potential use of sialic acid from edible bird’s nest to attenuate mitochondrial dysfunction by in vitro study
publisher Frontiers Research Foundation
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/95564/1/The%20potential%20use%20of%20sialic%20acid%20from%20edible%20bird%E2%80%99s%20nest%20to%20attenuate%20mitochondrial%20dysfunction%20by%20in%20vitro%20study.pdf
http://psasir.upm.edu.my/id/eprint/95564/
https://www.frontiersin.org/articles/10.3389/fphar.2021.633303/full
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score 13.211869

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