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Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli

Chilli (Capsicum annuum L.) is one of the major vegetable and spice crops grown widely, yet it suffers great losses due to infection by various viruses, including Cucumber Mosaic Virus (CMV). The conventional breeding of chilli hybrids for improved disease resistance is a long process with proble...

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Main Author: Lim, Jee Hian
Format: Thesis
Language:English
English
Published: 2003
Online Access:http://psasir.upm.edu.my/id/eprint/9538/1/FSAS_2003_7_A.pdf
http://psasir.upm.edu.my/id/eprint/9538/
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spelling my.upm.eprints.95382012-05-21T01:48:51Z http://psasir.upm.edu.my/id/eprint/9538/ Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli Lim, Jee Hian Chilli (Capsicum annuum L.) is one of the major vegetable and spice crops grown widely, yet it suffers great losses due to infection by various viruses, including Cucumber Mosaic Virus (CMV). The conventional breeding of chilli hybrids for improved disease resistance is a long process with problems of interspecific incompatibility. The objectives of the study were to construct a transforming vector containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) and development of an Agrobacterium-transformation procedure which can offer solutions to these problems. This study was approached primarily from the molecular aspect which the construct of a CMV CP has been successfully cloned. The cloned fragments were 655 bp and exhibited more than 90% similarity to those published CMV CP gene sequences. SDS-PAGE analysis showed the size of the expressed coat protein to be similar to the predicted size of 30 kD based on the DNA sequences. Western blot analysis was also conducted to confirm the bacterial expressed coat proteins. All these verified that the cloned genes were translatable CMV CP genes. 2003-05 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/9538/1/FSAS_2003_7_A.pdf Lim, Jee Hian (2003) Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli. Masters thesis, Universiti Putra Malaysia. English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Chilli (Capsicum annuum L.) is one of the major vegetable and spice crops grown widely, yet it suffers great losses due to infection by various viruses, including Cucumber Mosaic Virus (CMV). The conventional breeding of chilli hybrids for improved disease resistance is a long process with problems of interspecific incompatibility. The objectives of the study were to construct a transforming vector containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) and development of an Agrobacterium-transformation procedure which can offer solutions to these problems. This study was approached primarily from the molecular aspect which the construct of a CMV CP has been successfully cloned. The cloned fragments were 655 bp and exhibited more than 90% similarity to those published CMV CP gene sequences. SDS-PAGE analysis showed the size of the expressed coat protein to be similar to the predicted size of 30 kD based on the DNA sequences. Western blot analysis was also conducted to confirm the bacterial expressed coat proteins. All these verified that the cloned genes were translatable CMV CP genes.
format Thesis
author Lim, Jee Hian
spellingShingle Lim, Jee Hian
Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli
author_facet Lim, Jee Hian
author_sort Lim, Jee Hian
title Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli
title_short Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli
title_full Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli
title_fullStr Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli
title_full_unstemmed Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli
title_sort construction of a transformation vector containing cucumber mosaic virus (cmv) coat protein (cp) gene and development of an agrobacteriumtransformation procedure for chilli
publishDate 2003
url http://psasir.upm.edu.my/id/eprint/9538/1/FSAS_2003_7_A.pdf
http://psasir.upm.edu.my/id/eprint/9538/
_version_ 1643824344001937408
score 13.211869

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