Cover Image

Supplementation of postbiotic RI11 improves antioxidant enzymes activity, upregulated gut barrier genes and reduced cytokine, acute phase proteins and heat shock protein70 gene expression levels in heat-stressed broilers

The aim of this work was to evaluate the impacts of feeding different levels of postbiotic RI11 on antioxidant enzyme activity, physiological stress indicators, and cytokine and gut barrier gene expression in broilers under heat stress. A total of 252 male broilers Cobb 500 were allocated in cages i...

Full description

Saved in:
Bibliographic Details
Main Authors: Humam, Ali Merzza, Loh, Teck Chwen, Foo, Hooi Ling, Izuddin, Wan Ibrahim, Zulkifli, Idrus, Samsudin, Anjas Asmara, Mustapha, Noordin Mohamed
Format: Article
Published: Elsevier BV 2021
Online Access:http://psasir.upm.edu.my/id/eprint/95214/
https://www.sciencedirect.com/science/article/pii/S0032579120309597
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.95214
record_format eprints
spelling my.upm.eprints.952142023-02-03T08:37:10Z http://psasir.upm.edu.my/id/eprint/95214/ Supplementation of postbiotic RI11 improves antioxidant enzymes activity, upregulated gut barrier genes and reduced cytokine, acute phase proteins and heat shock protein70 gene expression levels in heat-stressed broilers Humam, Ali Merzza Loh, Teck Chwen Foo, Hooi Ling Izuddin, Wan Ibrahim Zulkifli, Idrus Samsudin, Anjas Asmara Mustapha, Noordin Mohamed The aim of this work was to evaluate the impacts of feeding different levels of postbiotic RI11 on antioxidant enzyme activity, physiological stress indicators, and cytokine and gut barrier gene expression in broilers under heat stress. A total of 252 male broilers Cobb 500 were allocated in cages in environmentally controlled chambers. All the broilers received the same basal diet from 1 to 21 d. On day 22, the broilers were weighed and grouped into 7 treatment groups and exhibited to cyclic high temperature at 36 ± 1°C for 3 h per day until the end of the experiment. From day 22 to 42, broilers were fed with one of the 7 following diets: negative control, basal diet (0.0% RI11) (NC group); positive control, NC diet + 0.02% (w/w) oxytetracycline (OTC group); antioxidant control, NC diet + 0.02% (w/w) ascorbic acid. The other 4 other groups were as follows: NC diet + 0.2% cell-free supernatant (postbiotic RI11) (v/w), NC diet + 0.4% cell-free supernatant (postbiotic RI11) (v/w), NC diet + 0.6% cell-free supernatant (postbiotic RI11) (v/w), and NC diet + 0.8% cell-free supernatant (postbiotic RI11) (v/w). Supplementation of different levels (0.4, 0.6, and 0.8%) of postbiotic RI11 increased plasma glutathione peroxidase, catalase, and glutathione enzyme activity. Postbiotic RI11 groups particularly at levels of 0.4 and 0.6% upregulated the mRNA expression of IL-10 and downregulated the IL-8, tumor necrosis factor alpha, heat shock protein 70, and alpha-1-acid glycoprotein levels compared with the NC and OTC groups. Feeding postbiotic RI11, particularly at the level of 0.6%, upregulated ileum zonula occludens-1 and mucin 2 mRNA expressions. However, no difference was observed in ileum claudin 1, ceruloplasmin, IL-6, IL-2, and interferon expression, but downregulation of occludin expression was observed as compared with the NC group. Supplementation of postbiotic RI11 at different levels quadratically increased plasma glutathione peroxidase, catalase and glutathione, IL-10, mucin 2, and zonula occludens-1 mRNA expression and reduced plasma IL-8, tumor necrosis factor alpha, alpha-1-acid glycoprotein, and heat shock protein 70 mRNA expression. The results suggested that postbiotics produced from Lactiplantibacillus plantarum RI11 especially at the level of 0.6% (v/w) could be used as an alternative to antibiotics and natural sources of antioxidants in poultry feeding. Elsevier BV 2021 Article PeerReviewed Humam, Ali Merzza and Loh, Teck Chwen and Foo, Hooi Ling and Izuddin, Wan Ibrahim and Zulkifli, Idrus and Samsudin, Anjas Asmara and Mustapha, Noordin Mohamed (2021) Supplementation of postbiotic RI11 improves antioxidant enzymes activity, upregulated gut barrier genes and reduced cytokine, acute phase proteins and heat shock protein70 gene expression levels in heat-stressed broilers. Poultry Science, 100 (3). pp. 1-24. ISSN 0032-5791; ESSN: 1525-3171 https://www.sciencedirect.com/science/article/pii/S0032579120309597 10.1016/j.psj.2020.12.011
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description The aim of this work was to evaluate the impacts of feeding different levels of postbiotic RI11 on antioxidant enzyme activity, physiological stress indicators, and cytokine and gut barrier gene expression in broilers under heat stress. A total of 252 male broilers Cobb 500 were allocated in cages in environmentally controlled chambers. All the broilers received the same basal diet from 1 to 21 d. On day 22, the broilers were weighed and grouped into 7 treatment groups and exhibited to cyclic high temperature at 36 ± 1°C for 3 h per day until the end of the experiment. From day 22 to 42, broilers were fed with one of the 7 following diets: negative control, basal diet (0.0% RI11) (NC group); positive control, NC diet + 0.02% (w/w) oxytetracycline (OTC group); antioxidant control, NC diet + 0.02% (w/w) ascorbic acid. The other 4 other groups were as follows: NC diet + 0.2% cell-free supernatant (postbiotic RI11) (v/w), NC diet + 0.4% cell-free supernatant (postbiotic RI11) (v/w), NC diet + 0.6% cell-free supernatant (postbiotic RI11) (v/w), and NC diet + 0.8% cell-free supernatant (postbiotic RI11) (v/w). Supplementation of different levels (0.4, 0.6, and 0.8%) of postbiotic RI11 increased plasma glutathione peroxidase, catalase, and glutathione enzyme activity. Postbiotic RI11 groups particularly at levels of 0.4 and 0.6% upregulated the mRNA expression of IL-10 and downregulated the IL-8, tumor necrosis factor alpha, heat shock protein 70, and alpha-1-acid glycoprotein levels compared with the NC and OTC groups. Feeding postbiotic RI11, particularly at the level of 0.6%, upregulated ileum zonula occludens-1 and mucin 2 mRNA expressions. However, no difference was observed in ileum claudin 1, ceruloplasmin, IL-6, IL-2, and interferon expression, but downregulation of occludin expression was observed as compared with the NC group. Supplementation of postbiotic RI11 at different levels quadratically increased plasma glutathione peroxidase, catalase and glutathione, IL-10, mucin 2, and zonula occludens-1 mRNA expression and reduced plasma IL-8, tumor necrosis factor alpha, alpha-1-acid glycoprotein, and heat shock protein 70 mRNA expression. The results suggested that postbiotics produced from Lactiplantibacillus plantarum RI11 especially at the level of 0.6% (v/w) could be used as an alternative to antibiotics and natural sources of antioxidants in poultry feeding.
format Article
author Humam, Ali Merzza
Loh, Teck Chwen
Foo, Hooi Ling
Izuddin, Wan Ibrahim
Zulkifli, Idrus
Samsudin, Anjas Asmara
Mustapha, Noordin Mohamed
spellingShingle Humam, Ali Merzza
Loh, Teck Chwen
Foo, Hooi Ling
Izuddin, Wan Ibrahim
Zulkifli, Idrus
Samsudin, Anjas Asmara
Mustapha, Noordin Mohamed
Supplementation of postbiotic RI11 improves antioxidant enzymes activity, upregulated gut barrier genes and reduced cytokine, acute phase proteins and heat shock protein70 gene expression levels in heat-stressed broilers
author_facet Humam, Ali Merzza
Loh, Teck Chwen
Foo, Hooi Ling
Izuddin, Wan Ibrahim
Zulkifli, Idrus
Samsudin, Anjas Asmara
Mustapha, Noordin Mohamed
author_sort Humam, Ali Merzza
title Supplementation of postbiotic RI11 improves antioxidant enzymes activity, upregulated gut barrier genes and reduced cytokine, acute phase proteins and heat shock protein70 gene expression levels in heat-stressed broilers
title_short Supplementation of postbiotic RI11 improves antioxidant enzymes activity, upregulated gut barrier genes and reduced cytokine, acute phase proteins and heat shock protein70 gene expression levels in heat-stressed broilers
title_full Supplementation of postbiotic RI11 improves antioxidant enzymes activity, upregulated gut barrier genes and reduced cytokine, acute phase proteins and heat shock protein70 gene expression levels in heat-stressed broilers
title_fullStr Supplementation of postbiotic RI11 improves antioxidant enzymes activity, upregulated gut barrier genes and reduced cytokine, acute phase proteins and heat shock protein70 gene expression levels in heat-stressed broilers
title_full_unstemmed Supplementation of postbiotic RI11 improves antioxidant enzymes activity, upregulated gut barrier genes and reduced cytokine, acute phase proteins and heat shock protein70 gene expression levels in heat-stressed broilers
title_sort supplementation of postbiotic ri11 improves antioxidant enzymes activity, upregulated gut barrier genes and reduced cytokine, acute phase proteins and heat shock protein70 gene expression levels in heat-stressed broilers
publisher Elsevier BV
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/95214/
https://www.sciencedirect.com/science/article/pii/S0032579120309597
_version_ 1758579844239589376
score 13.211869

Similar Items