Cover Image

Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved

Colletotrichum gloeosporioides causes anthracnose disease in papaya fruit resulting in tremendous economic loss due to its latent infection. This study aimed to evaluate the biocontrol activity of antagonistic yeasts against C. gloeosporioides in papaya and determine the possible mechanism involved....

Full description

Saved in:
Bibliographic Details
Main Authors: Hassan, Hamizah, Tengku Muda Mohamed, Mahmud, Yusoff, Siti Fairuz, Mohd Hata, Erneeza, Tajidin, Nor Elliza
Format: Article
Published: MDPI AG 2021
Online Access:http://psasir.upm.edu.my/id/eprint/95030/
https://www.mdpi.com/2073-4395/11/4/760
https://www.mdpi.com/2073-4395/11/4/760
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.95030
record_format eprints
spelling my.upm.eprints.950302023-02-16T06:56:35Z http://psasir.upm.edu.my/id/eprint/95030/ Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved Hassan, Hamizah Tengku Muda Mohamed, Mahmud Yusoff, Siti Fairuz Mohd Hata, Erneeza Tajidin, Nor Elliza Colletotrichum gloeosporioides causes anthracnose disease in papaya fruit resulting in tremendous economic loss due to its latent infection. This study aimed to evaluate the biocontrol activity of antagonistic yeasts against C. gloeosporioides in papaya and determine the possible mechanism involved. One hundred and ten yeast strains were isolated from different parts of the papaya plant. Among them, only five strains, namely F001, F006, L003, FL013 and LP010, showed more than 55% radial growth inhibition of C. gloeosporioides. These five potent yeast strains were further evaluated in vitro and in vivo. The results indicated that strain F001 had the strongest biocontrol activity based on spore germination and fungal growth inhibition. In vivo, the strain F001 caused 66.7% and 25% reductions in disease incidence and severity, respectively. Based on molecular identification, the strain F001 was confirmed as Trichosporon asahii. Despite there was no significant induction of defense enzyme activities found on the treated fruits, SEM observation showed direct attachment of T. asahii with the fungal hyphae and interfere in their establishment to the fruit surface. Based on these findings, the antagonistic yeast T. asahii strain F001 may be used as a potential natural biological control agent against anthracnose disease in papaya fruit. MDPI AG 2021-04-13 Article PeerReviewed Hassan, Hamizah and Tengku Muda Mohamed, Mahmud and Yusoff, Siti Fairuz and Mohd Hata, Erneeza and Tajidin, Nor Elliza (2021) Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved. Agronomy, 11 (4). art. no. 760. pp. 1-17. ISSN 2073-4395 https://www.mdpi.com/2073-4395/11/4/760 https://www.mdpi.com/2073-4395/11/4/760
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Colletotrichum gloeosporioides causes anthracnose disease in papaya fruit resulting in tremendous economic loss due to its latent infection. This study aimed to evaluate the biocontrol activity of antagonistic yeasts against C. gloeosporioides in papaya and determine the possible mechanism involved. One hundred and ten yeast strains were isolated from different parts of the papaya plant. Among them, only five strains, namely F001, F006, L003, FL013 and LP010, showed more than 55% radial growth inhibition of C. gloeosporioides. These five potent yeast strains were further evaluated in vitro and in vivo. The results indicated that strain F001 had the strongest biocontrol activity based on spore germination and fungal growth inhibition. In vivo, the strain F001 caused 66.7% and 25% reductions in disease incidence and severity, respectively. Based on molecular identification, the strain F001 was confirmed as Trichosporon asahii. Despite there was no significant induction of defense enzyme activities found on the treated fruits, SEM observation showed direct attachment of T. asahii with the fungal hyphae and interfere in their establishment to the fruit surface. Based on these findings, the antagonistic yeast T. asahii strain F001 may be used as a potential natural biological control agent against anthracnose disease in papaya fruit.
format Article
author Hassan, Hamizah
Tengku Muda Mohamed, Mahmud
Yusoff, Siti Fairuz
Mohd Hata, Erneeza
Tajidin, Nor Elliza
spellingShingle Hassan, Hamizah
Tengku Muda Mohamed, Mahmud
Yusoff, Siti Fairuz
Mohd Hata, Erneeza
Tajidin, Nor Elliza
Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved
author_facet Hassan, Hamizah
Tengku Muda Mohamed, Mahmud
Yusoff, Siti Fairuz
Mohd Hata, Erneeza
Tajidin, Nor Elliza
author_sort Hassan, Hamizah
title Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved
title_short Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved
title_full Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved
title_fullStr Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved
title_full_unstemmed Selecting antagonistic yeast for postharvest biocontrol of Colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved
title_sort selecting antagonistic yeast for postharvest biocontrol of colletotrichum gloeosporioides in papaya fruit and possible mechanisms involved
publisher MDPI AG
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/95030/
https://www.mdpi.com/2073-4395/11/4/760
https://www.mdpi.com/2073-4395/11/4/760
_version_ 1758579838782799872
score 13.211869

Similar Items