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Pathological changes, distribution and detection of Brucella melitensis in foetuses of experimentally- infected does

Background: Brucellosis of goats is caused by Brucella melitensis. It is a re-emerging zoonotic disease in many countries due to transmission from domestic animals and wildlife such as ibex, deer and wild buffaloes. Objective: To describe the pathological changes, identification and distribution of...

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Main Authors: Mazlan, Mazlina, Bejo, Siti Khairani, Hamzah, Hazilawati, Nasruddin, Nurrul Shaqinah, Salleh, Annas, Saad, Mohd Zamri
Format: Article
Published: Taylor & Francis 2021
Online Access:http://psasir.upm.edu.my/id/eprint/94499/
https://www.tandfonline.com/doi/full/10.1080/01652176.2020.1867328
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spelling my.upm.eprints.944992022-11-29T07:33:25Z http://psasir.upm.edu.my/id/eprint/94499/ Pathological changes, distribution and detection of Brucella melitensis in foetuses of experimentally- infected does Mazlan, Mazlina Bejo, Siti Khairani Hamzah, Hazilawati Nasruddin, Nurrul Shaqinah Salleh, Annas Saad, Mohd Zamri Background: Brucellosis of goats is caused by Brucella melitensis. It is a re-emerging zoonotic disease in many countries due to transmission from domestic animals and wildlife such as ibex, deer and wild buffaloes. Objective: To describe the pathological changes, identification and distribution of B. melitensis in foetuses of experimentally infected does. Methods: Twelve female goats of approximately 90 days pregnant were divided into 4 groups. Group 1 was exposed intra-conjunctival to 100 mL of sterile PBS while goats of Groups 2, 3 and 4 were similarly exposed to 100 mL of an inoculum containing 109 CFU/mL of live B. melitensis. Goats of these groups were killed at 15, 30 and 60 days post-inoculation, respectively. Foetal fluid and tissues were collected for bacterial identification (using direct bacterial culture, PCR and immuno-peroxidase staining) and histopathological examination. Results: Bilateral intra-conjunctival exposure of pregnant does resulted in in-utero infection of the foetuses. All full-term foetuses of group 4 were either aborted or stillborn, showing petechiations of the skin or absence of hair coat with subcutaneous oedema. The internal organs showed most severe lesions. Immune-peroxidase staining revealed antigen distribution in all organs that became most extensive in group 4. Brucella melitensis was successfully isolated from the stomach content, foetal fluid and various other organs. Conclusion: Vertical transmission of caprine brucellosis was evident causing mild to moderate lesions in different organs. The samples of choice for isolation and identification of B. melitensis are stomach content as well as liver and spleen tissue. Taylor & Francis 2021-01 Article PeerReviewed Mazlan, Mazlina and Bejo, Siti Khairani and Hamzah, Hazilawati and Nasruddin, Nurrul Shaqinah and Salleh, Annas and Saad, Mohd Zamri (2021) Pathological changes, distribution and detection of Brucella melitensis in foetuses of experimentally- infected does. Veterinary Quarterly, 41 (1). 36 - 49. ISSN 0165-2176; ESSN: 1875-5941 https://www.tandfonline.com/doi/full/10.1080/01652176.2020.1867328 10.1080/01652176.2020.1867328
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Background: Brucellosis of goats is caused by Brucella melitensis. It is a re-emerging zoonotic disease in many countries due to transmission from domestic animals and wildlife such as ibex, deer and wild buffaloes. Objective: To describe the pathological changes, identification and distribution of B. melitensis in foetuses of experimentally infected does. Methods: Twelve female goats of approximately 90 days pregnant were divided into 4 groups. Group 1 was exposed intra-conjunctival to 100 mL of sterile PBS while goats of Groups 2, 3 and 4 were similarly exposed to 100 mL of an inoculum containing 109 CFU/mL of live B. melitensis. Goats of these groups were killed at 15, 30 and 60 days post-inoculation, respectively. Foetal fluid and tissues were collected for bacterial identification (using direct bacterial culture, PCR and immuno-peroxidase staining) and histopathological examination. Results: Bilateral intra-conjunctival exposure of pregnant does resulted in in-utero infection of the foetuses. All full-term foetuses of group 4 were either aborted or stillborn, showing petechiations of the skin or absence of hair coat with subcutaneous oedema. The internal organs showed most severe lesions. Immune-peroxidase staining revealed antigen distribution in all organs that became most extensive in group 4. Brucella melitensis was successfully isolated from the stomach content, foetal fluid and various other organs. Conclusion: Vertical transmission of caprine brucellosis was evident causing mild to moderate lesions in different organs. The samples of choice for isolation and identification of B. melitensis are stomach content as well as liver and spleen tissue.
format Article
author Mazlan, Mazlina
Bejo, Siti Khairani
Hamzah, Hazilawati
Nasruddin, Nurrul Shaqinah
Salleh, Annas
Saad, Mohd Zamri
spellingShingle Mazlan, Mazlina
Bejo, Siti Khairani
Hamzah, Hazilawati
Nasruddin, Nurrul Shaqinah
Salleh, Annas
Saad, Mohd Zamri
Pathological changes, distribution and detection of Brucella melitensis in foetuses of experimentally- infected does
author_facet Mazlan, Mazlina
Bejo, Siti Khairani
Hamzah, Hazilawati
Nasruddin, Nurrul Shaqinah
Salleh, Annas
Saad, Mohd Zamri
author_sort Mazlan, Mazlina
title Pathological changes, distribution and detection of Brucella melitensis in foetuses of experimentally- infected does
title_short Pathological changes, distribution and detection of Brucella melitensis in foetuses of experimentally- infected does
title_full Pathological changes, distribution and detection of Brucella melitensis in foetuses of experimentally- infected does
title_fullStr Pathological changes, distribution and detection of Brucella melitensis in foetuses of experimentally- infected does
title_full_unstemmed Pathological changes, distribution and detection of Brucella melitensis in foetuses of experimentally- infected does
title_sort pathological changes, distribution and detection of brucella melitensis in foetuses of experimentally- infected does
publisher Taylor & Francis
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/94499/
https://www.tandfonline.com/doi/full/10.1080/01652176.2020.1867328
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score 13.211869

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