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Utilization of glucose recovered by phase separation system from acid-hydrolysed oil palm empty fruit bunch for bioethanol production

Oil palm empty fruit bunch (OPEFB) is one the most abundant lignocellulosic wastes produced throughoutthe year in the palm oil industry. A new process of separating lignocellulose components after acid hydrolysis(known as phase separation system) has been previously developed, by which lignin and ca...

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Main Authors: Mohd Zainudin, Mohd Huzairi, Abdul Rahman, Nor'aini, Abd. Aziz, Suraini, Funaoka, Masamitsu, Shinano, Takanori, Shirai, Yoshihito, Wakisaka, Minato, Hassan, Mohd Ali
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2012
Online Access:http://psasir.upm.edu.my/id/eprint/944/1/Utilization%20of%20Glucose%20Recovered%20by%20Phase%20Separation%20System%20from%20Acid-hydrolysed%20Oil%20Palm%20Empty%20Fruit%20Bunch%20for%20Bioethanol%20Production.pdf
http://psasir.upm.edu.my/id/eprint/944/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2035%20(1)%20Feb.%202012/19%20Pg%20117-126.pdf
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spelling my.upm.eprints.9442016-09-02T07:48:05Z http://psasir.upm.edu.my/id/eprint/944/ Utilization of glucose recovered by phase separation system from acid-hydrolysed oil palm empty fruit bunch for bioethanol production Mohd Zainudin, Mohd Huzairi Abdul Rahman, Nor'aini Abd. Aziz, Suraini Funaoka, Masamitsu Shinano, Takanori Shirai, Yoshihito Wakisaka, Minato Hassan, Mohd Ali Oil palm empty fruit bunch (OPEFB) is one the most abundant lignocellulosic wastes produced throughoutthe year in the palm oil industry. A new process of separating lignocellulose components after acid hydrolysis(known as phase separation system) has been previously developed, by which lignin and carbohydrate can becompletely and rapidly separated in 60 minutes between 25 and 30°C. In this process, cellulose is completelyhydrolyzed to oligosaccharides and remains in the acid phase. The maximum glucose yield of 53.8% wasobtained by hydrolysis, with 4% acid after autoclaving at 121°C for 5 minutes. This work focused on theseparation of monosaccharide (glucose) from cellulose fraction, which was subsequently used as a substratefor ethanol production. For this purpose, different types of nitrogen sources were evaluated, with yeast extractas the best nitrogen source (93% of theoretical yield) as compared to palm oil mill effluent (POME) andsludge powder for the growth of acid tolerant Saccharomyces cerevisiae ATCC 26602. Batch and repeatedbatch fermentation of S. cerevisiae ATCC 26602 using OPEFB hydrolysate gave 0.46 g glucose g ethanol-1,representing 87% of theoretical yield with a productivity of about 0.82 g-1 l-1 h-1 and 0.48 g glucose g ethanol-1,representing 89% of theoretical yield with productivity of about 2.79 g-1 l-1 h-1, respectively. Universiti Putra Malaysia Press 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/944/1/Utilization%20of%20Glucose%20Recovered%20by%20Phase%20Separation%20System%20from%20Acid-hydrolysed%20Oil%20Palm%20Empty%20Fruit%20Bunch%20for%20Bioethanol%20Production.pdf Mohd Zainudin, Mohd Huzairi and Abdul Rahman, Nor'aini and Abd. Aziz, Suraini and Funaoka, Masamitsu and Shinano, Takanori and Shirai, Yoshihito and Wakisaka, Minato and Hassan, Mohd Ali (2012) Utilization of glucose recovered by phase separation system from acid-hydrolysed oil palm empty fruit bunch for bioethanol production. Pertanika Journal of Tropical Agricultural Science, 35 (1). pp. 117-126. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2035%20(1)%20Feb.%202012/19%20Pg%20117-126.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Oil palm empty fruit bunch (OPEFB) is one the most abundant lignocellulosic wastes produced throughoutthe year in the palm oil industry. A new process of separating lignocellulose components after acid hydrolysis(known as phase separation system) has been previously developed, by which lignin and carbohydrate can becompletely and rapidly separated in 60 minutes between 25 and 30°C. In this process, cellulose is completelyhydrolyzed to oligosaccharides and remains in the acid phase. The maximum glucose yield of 53.8% wasobtained by hydrolysis, with 4% acid after autoclaving at 121°C for 5 minutes. This work focused on theseparation of monosaccharide (glucose) from cellulose fraction, which was subsequently used as a substratefor ethanol production. For this purpose, different types of nitrogen sources were evaluated, with yeast extractas the best nitrogen source (93% of theoretical yield) as compared to palm oil mill effluent (POME) andsludge powder for the growth of acid tolerant Saccharomyces cerevisiae ATCC 26602. Batch and repeatedbatch fermentation of S. cerevisiae ATCC 26602 using OPEFB hydrolysate gave 0.46 g glucose g ethanol-1,representing 87% of theoretical yield with a productivity of about 0.82 g-1 l-1 h-1 and 0.48 g glucose g ethanol-1,representing 89% of theoretical yield with productivity of about 2.79 g-1 l-1 h-1, respectively.
format Article
author Mohd Zainudin, Mohd Huzairi
Abdul Rahman, Nor'aini
Abd. Aziz, Suraini
Funaoka, Masamitsu
Shinano, Takanori
Shirai, Yoshihito
Wakisaka, Minato
Hassan, Mohd Ali
spellingShingle Mohd Zainudin, Mohd Huzairi
Abdul Rahman, Nor'aini
Abd. Aziz, Suraini
Funaoka, Masamitsu
Shinano, Takanori
Shirai, Yoshihito
Wakisaka, Minato
Hassan, Mohd Ali
Utilization of glucose recovered by phase separation system from acid-hydrolysed oil palm empty fruit bunch for bioethanol production
author_facet Mohd Zainudin, Mohd Huzairi
Abdul Rahman, Nor'aini
Abd. Aziz, Suraini
Funaoka, Masamitsu
Shinano, Takanori
Shirai, Yoshihito
Wakisaka, Minato
Hassan, Mohd Ali
author_sort Mohd Zainudin, Mohd Huzairi
title Utilization of glucose recovered by phase separation system from acid-hydrolysed oil palm empty fruit bunch for bioethanol production
title_short Utilization of glucose recovered by phase separation system from acid-hydrolysed oil palm empty fruit bunch for bioethanol production
title_full Utilization of glucose recovered by phase separation system from acid-hydrolysed oil palm empty fruit bunch for bioethanol production
title_fullStr Utilization of glucose recovered by phase separation system from acid-hydrolysed oil palm empty fruit bunch for bioethanol production
title_full_unstemmed Utilization of glucose recovered by phase separation system from acid-hydrolysed oil palm empty fruit bunch for bioethanol production
title_sort utilization of glucose recovered by phase separation system from acid-hydrolysed oil palm empty fruit bunch for bioethanol production
publisher Universiti Putra Malaysia Press
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/944/1/Utilization%20of%20Glucose%20Recovered%20by%20Phase%20Separation%20System%20from%20Acid-hydrolysed%20Oil%20Palm%20Empty%20Fruit%20Bunch%20for%20Bioethanol%20Production.pdf
http://psasir.upm.edu.my/id/eprint/944/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2035%20(1)%20Feb.%202012/19%20Pg%20117-126.pdf
_version_ 1643821931085955072
score 13.211869

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