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NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model

Mice have served as an excellent model to understand the etiology of lung cancer for years. However, data regarding dual-stage carcinogenesis of lung squamous cell carcinoma (SCC) remain elusive. Therefore, we aim to develop pre-malignant (PM) and malignant (M) lung SCC in vivo using N-nitroso-tris-...

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Main Authors: Zakaria, Muhammad Asyaari, Rajab, Nor Fadilah, Eng, Wee Chua, Selvarajah, Gayathri Thevi, Masre, Siti Fathiah
Format: Article
Published: Nature Publishing Group 2021
Online Access:http://psasir.upm.edu.my/id/eprint/94386/
https://www.nature.com/articles/s41598-021-01988-8
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spelling my.upm.eprints.943862023-04-04T08:04:41Z http://psasir.upm.edu.my/id/eprint/94386/ NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model Zakaria, Muhammad Asyaari Rajab, Nor Fadilah Eng, Wee Chua Selvarajah, Gayathri Thevi Masre, Siti Fathiah Mice have served as an excellent model to understand the etiology of lung cancer for years. However, data regarding dual-stage carcinogenesis of lung squamous cell carcinoma (SCC) remain elusive. Therefore, we aim to develop pre-malignant (PM) and malignant (M) lung SCC in vivo using N-nitroso-tris-chloroethylurea (NTCU). BALB/C mice were allotted into two main groups; PM and M groups which received treatment for 15 and 30 weeks, respectively. Then, the mice in each main group were allotted into three groups; control, vehicle, and cancer (n = 6), which received normal saline, 70% acetone, and 0.04 M NTCU by skin painting, respectively. Histopathologically, we discovered a mix of hyperplasia, metaplasia, and dysplasia lesions in the PM group and intracellular bridge; an SCC feature in the M group. The M group was positive for cytokeratin 5/6 protein which confirmed the lung SCC subtype. We also found significantly higher (P < 0.05) epithelium thickness in the cancer groups as compared to the vehicle and control groups at both the PM and M. Overall, this study discovered that NTCU is capable of developing PM and M lung SCC in mice model at appropriate weeks and the vehicle group was suggested to be adequate as control group for future research. Nature Publishing Group 2021-11-18 Article PeerReviewed Zakaria, Muhammad Asyaari and Rajab, Nor Fadilah and Eng, Wee Chua and Selvarajah, Gayathri Thevi and Masre, Siti Fathiah (2021) NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model. Scientific Reports, 11. art. no. 22500. pp. 1-10. ISSN 2045-2322 https://www.nature.com/articles/s41598-021-01988-8 10.1038/s41598-021-01988-8
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Mice have served as an excellent model to understand the etiology of lung cancer for years. However, data regarding dual-stage carcinogenesis of lung squamous cell carcinoma (SCC) remain elusive. Therefore, we aim to develop pre-malignant (PM) and malignant (M) lung SCC in vivo using N-nitroso-tris-chloroethylurea (NTCU). BALB/C mice were allotted into two main groups; PM and M groups which received treatment for 15 and 30 weeks, respectively. Then, the mice in each main group were allotted into three groups; control, vehicle, and cancer (n = 6), which received normal saline, 70% acetone, and 0.04 M NTCU by skin painting, respectively. Histopathologically, we discovered a mix of hyperplasia, metaplasia, and dysplasia lesions in the PM group and intracellular bridge; an SCC feature in the M group. The M group was positive for cytokeratin 5/6 protein which confirmed the lung SCC subtype. We also found significantly higher (P < 0.05) epithelium thickness in the cancer groups as compared to the vehicle and control groups at both the PM and M. Overall, this study discovered that NTCU is capable of developing PM and M lung SCC in mice model at appropriate weeks and the vehicle group was suggested to be adequate as control group for future research.
format Article
author Zakaria, Muhammad Asyaari
Rajab, Nor Fadilah
Eng, Wee Chua
Selvarajah, Gayathri Thevi
Masre, Siti Fathiah
spellingShingle Zakaria, Muhammad Asyaari
Rajab, Nor Fadilah
Eng, Wee Chua
Selvarajah, Gayathri Thevi
Masre, Siti Fathiah
NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model
author_facet Zakaria, Muhammad Asyaari
Rajab, Nor Fadilah
Eng, Wee Chua
Selvarajah, Gayathri Thevi
Masre, Siti Fathiah
author_sort Zakaria, Muhammad Asyaari
title NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model
title_short NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model
title_full NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model
title_fullStr NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model
title_full_unstemmed NTCU induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model
title_sort ntcu induced pre-malignant and malignant stages of lung squamous cell carcinoma in mice model
publisher Nature Publishing Group
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/94386/
https://www.nature.com/articles/s41598-021-01988-8
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