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Pathogenesis and vertical transmission of a transplacental rat cytomegalovirus

Background Cytomegalovirus (CMV) congenital infection is the major viral cause of well-documented birth defects in human. Because CMV is species-specific, the main obstacle to developing animal models for congenital infection is the difference in placental architecture, which preludes virus trans...

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Main Authors: Loh, Hwei San, Mohd Lila, Mohd Azmi, Abdul Rahman, Sheikh Omar, Kiew, Lik Jun
Format: Article
Language:English
English
Published: Springer 2006
Online Access:http://psasir.upm.edu.my/id/eprint/9426/1/Pathogenesis%20and%20vertical%20transmission%20of%20a%20transplacental%20rat%20cytomegalovirus.pdf
http://psasir.upm.edu.my/id/eprint/9426/
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spelling my.upm.eprints.94262015-10-05T23:57:13Z http://psasir.upm.edu.my/id/eprint/9426/ Pathogenesis and vertical transmission of a transplacental rat cytomegalovirus Loh, Hwei San Mohd Lila, Mohd Azmi Abdul Rahman, Sheikh Omar Kiew, Lik Jun Background Cytomegalovirus (CMV) congenital infection is the major viral cause of well-documented birth defects in human. Because CMV is species-specific, the main obstacle to developing animal models for congenital infection is the difference in placental architecture, which preludes virus transmission across the placenta. The rat placenta, resembling histologically to that of human, could therefore facilitate the study of CMV congenital infection in human. Results In this report, we present clear evidences of the transplacental property of a new rat CMV (RCMV), namely ALL-03, which had been isolated from placenta and uterus of the house rat. Our study signifies the detection of infectious virus, virus particles, viral protein and DNA as well as immune response to demonstrate a natural model of acute CMV infection including the immunocompetent and immunocompromised host associated with or without pregnancy. It is characterized by a full range of CMV related clinical signs; lesions and anatomical virus distribution to uterus, placenta, embryo, fetus, neonate, lung, kidney, spleen, liver and salivary gland of the infected rats in addition to the virus-specific seroconversion. The preference of the virus for different organs mimics the situation in immunocompromised man. Most interestingly, the placenta was observed to be involved in the maternofetal infection and hence confirmed the hypothesis that the RCMV strain ALL-03 is capable to cross the placenta and infect the offsprings congenitally. Conclusion The maternal viremia leading to uterine infection which subsequently infecting to the fetus through the placenta is the most likely phenomenon of CMV vertical transmission in our study. Springer 2006-06-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/9426/1/Pathogenesis%20and%20vertical%20transmission%20of%20a%20transplacental%20rat%20cytomegalovirus.pdf Loh, Hwei San and Mohd Lila, Mohd Azmi and Abdul Rahman, Sheikh Omar and Kiew, Lik Jun (2006) Pathogenesis and vertical transmission of a transplacental rat cytomegalovirus. Virology Journal, 3 (42). pp. 1-14. ISSN 1743-422x 10.1186/1743-422X-3-42 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Background Cytomegalovirus (CMV) congenital infection is the major viral cause of well-documented birth defects in human. Because CMV is species-specific, the main obstacle to developing animal models for congenital infection is the difference in placental architecture, which preludes virus transmission across the placenta. The rat placenta, resembling histologically to that of human, could therefore facilitate the study of CMV congenital infection in human. Results In this report, we present clear evidences of the transplacental property of a new rat CMV (RCMV), namely ALL-03, which had been isolated from placenta and uterus of the house rat. Our study signifies the detection of infectious virus, virus particles, viral protein and DNA as well as immune response to demonstrate a natural model of acute CMV infection including the immunocompetent and immunocompromised host associated with or without pregnancy. It is characterized by a full range of CMV related clinical signs; lesions and anatomical virus distribution to uterus, placenta, embryo, fetus, neonate, lung, kidney, spleen, liver and salivary gland of the infected rats in addition to the virus-specific seroconversion. The preference of the virus for different organs mimics the situation in immunocompromised man. Most interestingly, the placenta was observed to be involved in the maternofetal infection and hence confirmed the hypothesis that the RCMV strain ALL-03 is capable to cross the placenta and infect the offsprings congenitally. Conclusion The maternal viremia leading to uterine infection which subsequently infecting to the fetus through the placenta is the most likely phenomenon of CMV vertical transmission in our study.
format Article
author Loh, Hwei San
Mohd Lila, Mohd Azmi
Abdul Rahman, Sheikh Omar
Kiew, Lik Jun
spellingShingle Loh, Hwei San
Mohd Lila, Mohd Azmi
Abdul Rahman, Sheikh Omar
Kiew, Lik Jun
Pathogenesis and vertical transmission of a transplacental rat cytomegalovirus
author_facet Loh, Hwei San
Mohd Lila, Mohd Azmi
Abdul Rahman, Sheikh Omar
Kiew, Lik Jun
author_sort Loh, Hwei San
title Pathogenesis and vertical transmission of a transplacental rat cytomegalovirus
title_short Pathogenesis and vertical transmission of a transplacental rat cytomegalovirus
title_full Pathogenesis and vertical transmission of a transplacental rat cytomegalovirus
title_fullStr Pathogenesis and vertical transmission of a transplacental rat cytomegalovirus
title_full_unstemmed Pathogenesis and vertical transmission of a transplacental rat cytomegalovirus
title_sort pathogenesis and vertical transmission of a transplacental rat cytomegalovirus
publisher Springer
publishDate 2006
url http://psasir.upm.edu.my/id/eprint/9426/1/Pathogenesis%20and%20vertical%20transmission%20of%20a%20transplacental%20rat%20cytomegalovirus.pdf
http://psasir.upm.edu.my/id/eprint/9426/
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score 13.211869

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