Cover Image

Molybdate reduction to molybdenum blue and growth on polyethylene glycol by Bacillus sp. strain Neni-8

The accumulation of heavy metals and xenobiotic compounds in soil and aquatic bodies is caused by inappropriate waste disposal, industrial and mining operations, and excessive use of agricultural pesticides. Bioremediation is a more cost-effective way of removing these pollutants than other approach...

Full description

Saved in:
Bibliographic Details
Main Authors: Rusnam, Rahman, Mohd Fadhil, Gusmanizar, Neni, Yakasai, Hafeez Muhammad, Shukor, Mohd Yunus
Format: Article
Published: Hibiscus Publisher Enterprise 2021
Online Access:http://psasir.upm.edu.my/id/eprint/94259/
https://journal.hibiscuspublisher.com/index.php/BESSM/article/view/586
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.94259
record_format eprints
spelling my.upm.eprints.942592023-05-08T04:13:28Z http://psasir.upm.edu.my/id/eprint/94259/ Molybdate reduction to molybdenum blue and growth on polyethylene glycol by Bacillus sp. strain Neni-8 Rusnam Rahman, Mohd Fadhil Gusmanizar, Neni Yakasai, Hafeez Muhammad Shukor, Mohd Yunus The accumulation of heavy metals and xenobiotic compounds in soil and aquatic bodies is caused by inappropriate waste disposal, industrial and mining operations, and excessive use of agricultural pesticides. Bioremediation is a more cost-effective way of removing these pollutants than other approaches. A new molybdenum-reducing bacterium with the ability to grow on a variety of polyethylene glycol (PEG)s has been discovered. Based on biochemical test, the bacterium was partially identified as Bacillus sp. strain Neni-8. Mo-blue production required an optimal pH of between 6.3 and 6.5, and between 30 and 37 oC. The carbon source, D-glucose best supported molybdenum reduction. A narrow requirement for phosphate of between 2.5 and 7.5 mM for molybdenum reduction was seen. Sodium molybdate as a substrate for reduction showed maximal reduction between 20 and 30 mM. The molybdenum blue absorption spectrum indicates that its identity was possibly a reduced phosphomolybdate. Several heavy metals such as silver, mercury, copper and chromium inhibited molybdenum reduction by 67.6, 48.7, 36.8 and 17.4 %, respectively. Bacterial growth modelled using the modified Gompertz model with PEG 600 as the best carbon source predicted a maximum growth rate of 15.4 Ln CFU/ml, a maximum specific growth rate of 0.198 h-1 and a lag period of 10.1 h. The novel characteristics of this bacterium are very useful in future bioremediation works. Hibiscus Publisher Enterprise 2021-07-31 Article PeerReviewed Rusnam and Rahman, Mohd Fadhil and Gusmanizar, Neni and Yakasai, Hafeez Muhammad and Shukor, Mohd Yunus (2021) Molybdate reduction to molybdenum blue and growth on polyethylene glycol by Bacillus sp. strain Neni-8. Bulletin of Environmental Science & Sustainable Management, 5 (1). pp. 12-19. ISSN 2716-5353 https://journal.hibiscuspublisher.com/index.php/BESSM/article/view/586 10.54987/bessm.v5i1.586
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description The accumulation of heavy metals and xenobiotic compounds in soil and aquatic bodies is caused by inappropriate waste disposal, industrial and mining operations, and excessive use of agricultural pesticides. Bioremediation is a more cost-effective way of removing these pollutants than other approaches. A new molybdenum-reducing bacterium with the ability to grow on a variety of polyethylene glycol (PEG)s has been discovered. Based on biochemical test, the bacterium was partially identified as Bacillus sp. strain Neni-8. Mo-blue production required an optimal pH of between 6.3 and 6.5, and between 30 and 37 oC. The carbon source, D-glucose best supported molybdenum reduction. A narrow requirement for phosphate of between 2.5 and 7.5 mM for molybdenum reduction was seen. Sodium molybdate as a substrate for reduction showed maximal reduction between 20 and 30 mM. The molybdenum blue absorption spectrum indicates that its identity was possibly a reduced phosphomolybdate. Several heavy metals such as silver, mercury, copper and chromium inhibited molybdenum reduction by 67.6, 48.7, 36.8 and 17.4 %, respectively. Bacterial growth modelled using the modified Gompertz model with PEG 600 as the best carbon source predicted a maximum growth rate of 15.4 Ln CFU/ml, a maximum specific growth rate of 0.198 h-1 and a lag period of 10.1 h. The novel characteristics of this bacterium are very useful in future bioremediation works.
format Article
author Rusnam
Rahman, Mohd Fadhil
Gusmanizar, Neni
Yakasai, Hafeez Muhammad
Shukor, Mohd Yunus
spellingShingle Rusnam
Rahman, Mohd Fadhil
Gusmanizar, Neni
Yakasai, Hafeez Muhammad
Shukor, Mohd Yunus
Molybdate reduction to molybdenum blue and growth on polyethylene glycol by Bacillus sp. strain Neni-8
author_facet Rusnam
Rahman, Mohd Fadhil
Gusmanizar, Neni
Yakasai, Hafeez Muhammad
Shukor, Mohd Yunus
author_sort Rusnam
title Molybdate reduction to molybdenum blue and growth on polyethylene glycol by Bacillus sp. strain Neni-8
title_short Molybdate reduction to molybdenum blue and growth on polyethylene glycol by Bacillus sp. strain Neni-8
title_full Molybdate reduction to molybdenum blue and growth on polyethylene glycol by Bacillus sp. strain Neni-8
title_fullStr Molybdate reduction to molybdenum blue and growth on polyethylene glycol by Bacillus sp. strain Neni-8
title_full_unstemmed Molybdate reduction to molybdenum blue and growth on polyethylene glycol by Bacillus sp. strain Neni-8
title_sort molybdate reduction to molybdenum blue and growth on polyethylene glycol by bacillus sp. strain neni-8
publisher Hibiscus Publisher Enterprise
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/94259/
https://journal.hibiscuspublisher.com/index.php/BESSM/article/view/586
_version_ 1768009394541297664
score 13.211869

Similar Items