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Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken

Background: Inclusion body hepatitis (IBH) is an economically important viral disease primarily affecting broiler and breeder chickens. All 12 serotypes of fowl adenovirus (FAdV) can cause IBH. Objectives: To characterize FAdV isolates based on phylogenetic analysis, and to study the pathogenicity...

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Main Authors: Sabarudin, Nur Syazana, Tan, Sheau Wei, Phang, Yuen Fun, Omar, Abdul Rahman
Format: Article
Published: Korean Society of Veterinary Science 2021
Online Access:http://psasir.upm.edu.my/id/eprint/94246/
https://www.vetsci.org/search.php?where=aview&id=10.4142/jvs.2021.22.e42&code=0118JVS&vmode=FULL
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spelling my.upm.eprints.942462023-05-09T01:58:58Z http://psasir.upm.edu.my/id/eprint/94246/ Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken Sabarudin, Nur Syazana Tan, Sheau Wei Phang, Yuen Fun Omar, Abdul Rahman Background: Inclusion body hepatitis (IBH) is an economically important viral disease primarily affecting broiler and breeder chickens. All 12 serotypes of fowl adenovirus (FAdV) can cause IBH. Objectives: To characterize FAdV isolates based on phylogenetic analysis, and to study the pathogenicity of FAdV-8b in specific-pathogen-free (SPF) chickens following virus inoculation via oral and intramuscular (IM) routes. Methods: Suspected organ samples were subjected to virus isolation and polymerase chain reaction (PCR) for FAdV detection. Hexon gene sequencing and phylogenetic analysis were performed on FAdV-positive samples for serotype identification. One FAdV-8b isolate, UPM/FAdV/420/2017, was selected for fiber gene characterization and pathogenicity study and was inoculated in SPF chickens via oral and IM routes. Results: The hexon gene phylogenetic analysis revealed that all isolates belonged to FAdV8b. The fiber gene-based phylogenetic analysis of isolate UPM/FAdV/420/2017 supported the grouping of that isolate into FAdV species E. Pathogenicity study revealed that, chickens infected with UPM/FAdV/420/2017 via the IM route had higher clinical score values, higher percent mortality, higher degree of the liver lesions, higher antibody response (p < 0.05), and higher virus shedding amounts (p < 0.05) than those infected via the oral route. The highest virus copy numbers were detected in liver and gizzard. Conclusions: FAdV-8b is the dominant FAdV serotype in Malaysia, and pathogenicity study of the FAdV-8b isolate UPM/FAdV/420/2017 indicated its ability to induce IBH in young SPF chickens when infected via oral or IM routes. Korean Society of Veterinary Science 2021-05-11 Article PeerReviewed Sabarudin, Nur Syazana and Tan, Sheau Wei and Phang, Yuen Fun and Omar, Abdul Rahman (2021) Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken. Journal of Veterinary Science, 22 (4). art. no. e42. pp. 1-16. ISSN 1229-845X; ESSN 1976-555X https://www.vetsci.org/search.php?where=aview&id=10.4142/jvs.2021.22.e42&code=0118JVS&vmode=FULL 10.4142/jvs.2021.22.e42
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Background: Inclusion body hepatitis (IBH) is an economically important viral disease primarily affecting broiler and breeder chickens. All 12 serotypes of fowl adenovirus (FAdV) can cause IBH. Objectives: To characterize FAdV isolates based on phylogenetic analysis, and to study the pathogenicity of FAdV-8b in specific-pathogen-free (SPF) chickens following virus inoculation via oral and intramuscular (IM) routes. Methods: Suspected organ samples were subjected to virus isolation and polymerase chain reaction (PCR) for FAdV detection. Hexon gene sequencing and phylogenetic analysis were performed on FAdV-positive samples for serotype identification. One FAdV-8b isolate, UPM/FAdV/420/2017, was selected for fiber gene characterization and pathogenicity study and was inoculated in SPF chickens via oral and IM routes. Results: The hexon gene phylogenetic analysis revealed that all isolates belonged to FAdV8b. The fiber gene-based phylogenetic analysis of isolate UPM/FAdV/420/2017 supported the grouping of that isolate into FAdV species E. Pathogenicity study revealed that, chickens infected with UPM/FAdV/420/2017 via the IM route had higher clinical score values, higher percent mortality, higher degree of the liver lesions, higher antibody response (p < 0.05), and higher virus shedding amounts (p < 0.05) than those infected via the oral route. The highest virus copy numbers were detected in liver and gizzard. Conclusions: FAdV-8b is the dominant FAdV serotype in Malaysia, and pathogenicity study of the FAdV-8b isolate UPM/FAdV/420/2017 indicated its ability to induce IBH in young SPF chickens when infected via oral or IM routes.
format Article
author Sabarudin, Nur Syazana
Tan, Sheau Wei
Phang, Yuen Fun
Omar, Abdul Rahman
spellingShingle Sabarudin, Nur Syazana
Tan, Sheau Wei
Phang, Yuen Fun
Omar, Abdul Rahman
Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken
author_facet Sabarudin, Nur Syazana
Tan, Sheau Wei
Phang, Yuen Fun
Omar, Abdul Rahman
author_sort Sabarudin, Nur Syazana
title Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken
title_short Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken
title_full Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken
title_fullStr Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken
title_full_unstemmed Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken
title_sort molecular characterization of malaysian fowl adenovirus (fadv) serotype 8b species e and pathogenicity of the virus in specific pathogen-free chicken
publisher Korean Society of Veterinary Science
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/94246/
https://www.vetsci.org/search.php?where=aview&id=10.4142/jvs.2021.22.e42&code=0118JVS&vmode=FULL
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score 13.211869

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