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Molecular and biological characterization of citrus bent leaf viroid from Malaysia

Background: A 328-nucleotide variant of citrus bent leaf viroid (CBLVd) was characterized by citrus varieties in Malaysia. After the first report in Malaysia, the emerging CBLVd was detected in five citrus species, namely Citrofortunella microcarpa, Citrus aurantifolia, Citrus hystrix, Citrus maxima...

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Main Authors: Iftikhar, Yasir, Khoo, Ying Wei, Murugan, Tanes, Roslin, Nor Athirah, Rabiatul Adawiyah, Kong, Lih Ling, Vadamalai, Ganesan
Format: Article
Published: Springer 2021
Online Access:http://psasir.upm.edu.my/id/eprint/94242/
https://link.springer.com/article/10.1007/s11033-021-06930-9
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spelling my.upm.eprints.942422023-05-09T01:55:26Z http://psasir.upm.edu.my/id/eprint/94242/ Molecular and biological characterization of citrus bent leaf viroid from Malaysia Iftikhar, Yasir Khoo, Ying Wei Murugan, Tanes Roslin, Nor Athirah Rabiatul Adawiyah Kong, Lih Ling Vadamalai, Ganesan Background: A 328-nucleotide variant of citrus bent leaf viroid (CBLVd) was characterized by citrus varieties in Malaysia. After the first report in Malaysia, the emerging CBLVd was detected in five citrus species, namely Citrofortunella microcarpa, Citrus aurantifolia, Citrus hystrix, Citrus maxima, and Citrus sinensis. Methods and results: CBLVd was detected in 23 out of 133 symptomatic samples through RT-PCR. Sequence analysis of the RT-PCR amplicons from this study showed 99–100% sequence identity to the reference CBLVd Jp isolate and CBLVd isolates reported in Malaysia. Inoculation of sap, obtained from a CBLVd positive sample, into 6-month old healthy C. microcarpa seedlings showed symptoms of slight leaf bending, reduced leaf size of matured leaves, and mild mosaic between 4 to 6 months after inoculation. Moreover, the observed symptoms of chlorosis, midvein necrosis, leaf rolling, and smalling of leaves in calamondin, C. microcarpa (Bunge) Wijnands, were not reported in earlier studies and opened a new avenue for the study of symptomology. The mechanical transmissibility of CBLVd in the inoculated seedlings was reconfirmed by RT-PCR assay and sequencing. Conclusions: Based on the results, the sequence similarity of CBLVd isolates from different areas of Malaysia showed no significant difference among each other and the reference isolate. The CBLVd is mechanically transmissible and could produce variable symptoms in different hosts. Springer 2021-11-13 Article PeerReviewed Iftikhar, Yasir and Khoo, Ying Wei and Murugan, Tanes and Roslin, Nor Athirah and Rabiatul Adawiyah and Kong, Lih Ling and Vadamalai, Ganesan (2021) Molecular and biological characterization of citrus bent leaf viroid from Malaysia. Molecular Biology Reports, 49. pp. 1581-1586. ISSN 0301-4851; ESSN:1573-4978 https://link.springer.com/article/10.1007/s11033-021-06930-9 10.1007/s11033-021-06930-9
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Background: A 328-nucleotide variant of citrus bent leaf viroid (CBLVd) was characterized by citrus varieties in Malaysia. After the first report in Malaysia, the emerging CBLVd was detected in five citrus species, namely Citrofortunella microcarpa, Citrus aurantifolia, Citrus hystrix, Citrus maxima, and Citrus sinensis. Methods and results: CBLVd was detected in 23 out of 133 symptomatic samples through RT-PCR. Sequence analysis of the RT-PCR amplicons from this study showed 99–100% sequence identity to the reference CBLVd Jp isolate and CBLVd isolates reported in Malaysia. Inoculation of sap, obtained from a CBLVd positive sample, into 6-month old healthy C. microcarpa seedlings showed symptoms of slight leaf bending, reduced leaf size of matured leaves, and mild mosaic between 4 to 6 months after inoculation. Moreover, the observed symptoms of chlorosis, midvein necrosis, leaf rolling, and smalling of leaves in calamondin, C. microcarpa (Bunge) Wijnands, were not reported in earlier studies and opened a new avenue for the study of symptomology. The mechanical transmissibility of CBLVd in the inoculated seedlings was reconfirmed by RT-PCR assay and sequencing. Conclusions: Based on the results, the sequence similarity of CBLVd isolates from different areas of Malaysia showed no significant difference among each other and the reference isolate. The CBLVd is mechanically transmissible and could produce variable symptoms in different hosts.
format Article
author Iftikhar, Yasir
Khoo, Ying Wei
Murugan, Tanes
Roslin, Nor Athirah
Rabiatul Adawiyah
Kong, Lih Ling
Vadamalai, Ganesan
spellingShingle Iftikhar, Yasir
Khoo, Ying Wei
Murugan, Tanes
Roslin, Nor Athirah
Rabiatul Adawiyah
Kong, Lih Ling
Vadamalai, Ganesan
Molecular and biological characterization of citrus bent leaf viroid from Malaysia
author_facet Iftikhar, Yasir
Khoo, Ying Wei
Murugan, Tanes
Roslin, Nor Athirah
Rabiatul Adawiyah
Kong, Lih Ling
Vadamalai, Ganesan
author_sort Iftikhar, Yasir
title Molecular and biological characterization of citrus bent leaf viroid from Malaysia
title_short Molecular and biological characterization of citrus bent leaf viroid from Malaysia
title_full Molecular and biological characterization of citrus bent leaf viroid from Malaysia
title_fullStr Molecular and biological characterization of citrus bent leaf viroid from Malaysia
title_full_unstemmed Molecular and biological characterization of citrus bent leaf viroid from Malaysia
title_sort molecular and biological characterization of citrus bent leaf viroid from malaysia
publisher Springer
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/94242/
https://link.springer.com/article/10.1007/s11033-021-06930-9
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score 13.211869

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