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Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP

The main objectives of this study are to evaluate the usefulness of a variety of recently developed DNA markers to identify and characterise different populations of Malaysian river catfish, Mystus numerus. In the initial methodology development, 111 primers comprising of 40 single RAPD primers,...

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Main Author: Chong, Lee Kim
Format: Thesis
Language:English
English
Published: 1998
Subjects:
DNA polymerases
Bagre - Malaysia
Online Access:http://psasir.upm.edu.my/id/eprint/9423/1/FSAS_1998_17_A.pdf
http://psasir.upm.edu.my/id/eprint/9423/
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spelling my.upm.eprints.94232024-02-29T08:21:35Z http://psasir.upm.edu.my/id/eprint/9423/ Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP Chong, Lee Kim The main objectives of this study are to evaluate the usefulness of a variety of recently developed DNA markers to identify and characterise different populations of Malaysian river catfish, Mystus numerus. In the initial methodology development, 111 primers comprising of 40 single RAPD primers, 64 pairs of AFLP primers and 7 pairs of African catfish microsatellite primers were screened against M. nemurus samples. RAPD and AFLP primers gave positive results. Both techniques were also successfully used in examining the genetic diversity present in five populations of M. nemurus originating from Kedah. Perak, Johor, Sarawak and one UPM culture population. However, none of the micro satellite primers developed for the African catfish was suitable for typing our local M. nemurus. Therefore, this technique was not used for the population studies and emphasis was only given to RAPD and AFLP markers in this thesis. Nine RAPD primers and four AFLP primers detected a total of 42 and 158 polymorphic markers respectively. The modes of inheritance of the bands produced by four out of the nine RAPD primers and two out of the four AFLP primers were studied using family samples. The results showed that 9 of the RAPD markers and 24 of the AFLP markers used in population studies segregated as stable Mendelian loci while 2 RAPD markers and 13 AFLP markers showed unusual segregation. The rest of the markers could not be examined because no segregation was found in the families. 1998-11 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/9423/1/FSAS_1998_17_A.pdf Chong, Lee Kim (1998) Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP. Masters thesis, Universiti Putra Malaysia. DNA polymerases Bagre - Malaysia English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
topic DNA polymerases
Bagre - Malaysia
spellingShingle DNA polymerases
Bagre - Malaysia
Chong, Lee Kim
Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP
description The main objectives of this study are to evaluate the usefulness of a variety of recently developed DNA markers to identify and characterise different populations of Malaysian river catfish, Mystus numerus. In the initial methodology development, 111 primers comprising of 40 single RAPD primers, 64 pairs of AFLP primers and 7 pairs of African catfish microsatellite primers were screened against M. nemurus samples. RAPD and AFLP primers gave positive results. Both techniques were also successfully used in examining the genetic diversity present in five populations of M. nemurus originating from Kedah. Perak, Johor, Sarawak and one UPM culture population. However, none of the micro satellite primers developed for the African catfish was suitable for typing our local M. nemurus. Therefore, this technique was not used for the population studies and emphasis was only given to RAPD and AFLP markers in this thesis. Nine RAPD primers and four AFLP primers detected a total of 42 and 158 polymorphic markers respectively. The modes of inheritance of the bands produced by four out of the nine RAPD primers and two out of the four AFLP primers were studied using family samples. The results showed that 9 of the RAPD markers and 24 of the AFLP markers used in population studies segregated as stable Mendelian loci while 2 RAPD markers and 13 AFLP markers showed unusual segregation. The rest of the markers could not be examined because no segregation was found in the families.
format Thesis
author Chong, Lee Kim
author_facet Chong, Lee Kim
author_sort Chong, Lee Kim
title Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP
title_short Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP
title_full Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP
title_fullStr Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP
title_full_unstemmed Development of PCR-BASED DNA Markers to Identify Aand Characterise Malaysian River Catfish, Mystus Nemurus (C&V) : RAPD and AFLP
title_sort development of pcr-based dna markers to identify aand characterise malaysian river catfish, mystus nemurus (c&v) : rapd and aflp
publishDate 1998
url http://psasir.upm.edu.my/id/eprint/9423/1/FSAS_1998_17_A.pdf
http://psasir.upm.edu.my/id/eprint/9423/
_version_ 1793156992200605696
score 13.211869

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