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Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization

In this study, a mixture of Tor tambra and T. tambroides with unknown genetic background were collected from 11 localities in Malaysia for broodstock development and sperm cryo-banking. This study aims to assess the microsatellite (simple sequence repeat, SSR) variation, genetic diversity, genetic d...

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Main Authors: Chew, Poh Chiang, Christianus, Annie, M. Zudaidy, Jaapar, Md Yasin, Ina-Salwany, Chong, Chou Min, Tan, Soon Guan
Format: Article
Published: MDPI 2021
Online Access:http://psasir.upm.edu.my/id/eprint/94187/
https://www.mdpi.com/2076-2615/11/9/2633
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spelling my.upm.eprints.941872023-05-09T03:12:13Z http://psasir.upm.edu.my/id/eprint/94187/ Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization Chew, Poh Chiang Christianus, Annie M. Zudaidy, Jaapar Md Yasin, Ina-Salwany Chong, Chou Min Tan, Soon Guan In this study, a mixture of Tor tambra and T. tambroides with unknown genetic background were collected from 11 localities in Malaysia for broodstock development and sperm cryo-banking. This study aims to assess the microsatellite (simple sequence repeat, SSR) variation, genetic diversity, genetic differentiation, level of gene flow, population structure, genetic relatedness and their demographic aspects among these Tor populations, in addition to establishing their SSR profile by employing 22 SSR markers via fragment analysis. Total genomic DNA was extracted from 181 samples (91 cryopreserved milt samples and 90 scale samples of live broodfish). Results showed the Tor spp. collection retained their genetic variation but exhibited excessive homozygosity among individuals within population. Moderate genetic differentiation was shown among the populations, with highly significant (p < 0.001) fixation indices (FST, FIS and FIT). A low gene flow over all loci (Nm 1.548) indicates little genetic variation transfer between populations. The genetic structures of all the populations were successfully resolved into four main clusters by an unweighted pair group method with arithmetic mean (UPGMA) dendrogram generated based on Nei’s genetic distances. The population structures based on principal coordinates analysis (PCoA) and the Bayesian model also suggested four distinct clusters following geographical regions and eight closely related populations. This study provided a useful baseline reference for better genetic management and utilization of the Tor spp. stocks in their breeding and conservation programmes. MDPI 2021-09-08 Article PeerReviewed Chew, Poh Chiang and Christianus, Annie and M. Zudaidy, Jaapar and Md Yasin, Ina-Salwany and Chong, Chou Min and Tan, Soon Guan (2021) Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization. Animals, 11 (9). art. no. 2633. pp. 1-28. ISSN 2076-2615 https://www.mdpi.com/2076-2615/11/9/2633 10.3390/ani11092633
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description In this study, a mixture of Tor tambra and T. tambroides with unknown genetic background were collected from 11 localities in Malaysia for broodstock development and sperm cryo-banking. This study aims to assess the microsatellite (simple sequence repeat, SSR) variation, genetic diversity, genetic differentiation, level of gene flow, population structure, genetic relatedness and their demographic aspects among these Tor populations, in addition to establishing their SSR profile by employing 22 SSR markers via fragment analysis. Total genomic DNA was extracted from 181 samples (91 cryopreserved milt samples and 90 scale samples of live broodfish). Results showed the Tor spp. collection retained their genetic variation but exhibited excessive homozygosity among individuals within population. Moderate genetic differentiation was shown among the populations, with highly significant (p < 0.001) fixation indices (FST, FIS and FIT). A low gene flow over all loci (Nm 1.548) indicates little genetic variation transfer between populations. The genetic structures of all the populations were successfully resolved into four main clusters by an unweighted pair group method with arithmetic mean (UPGMA) dendrogram generated based on Nei’s genetic distances. The population structures based on principal coordinates analysis (PCoA) and the Bayesian model also suggested four distinct clusters following geographical regions and eight closely related populations. This study provided a useful baseline reference for better genetic management and utilization of the Tor spp. stocks in their breeding and conservation programmes.
format Article
author Chew, Poh Chiang
Christianus, Annie
M. Zudaidy, Jaapar
Md Yasin, Ina-Salwany
Chong, Chou Min
Tan, Soon Guan
spellingShingle Chew, Poh Chiang
Christianus, Annie
M. Zudaidy, Jaapar
Md Yasin, Ina-Salwany
Chong, Chou Min
Tan, Soon Guan
Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization
author_facet Chew, Poh Chiang
Christianus, Annie
M. Zudaidy, Jaapar
Md Yasin, Ina-Salwany
Chong, Chou Min
Tan, Soon Guan
author_sort Chew, Poh Chiang
title Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization
title_short Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization
title_full Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization
title_fullStr Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization
title_full_unstemmed Microsatellite characterization of Malaysian mahseer (Tor spp.) for improvement of broodstock management and utilization
title_sort microsatellite characterization of malaysian mahseer (tor spp.) for improvement of broodstock management and utilization
publisher MDPI
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/94187/
https://www.mdpi.com/2076-2615/11/9/2633
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score 13.226497

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