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Metagenomic characterization of indoor dust fungal associated with allergy and lung inflammation among school children

The exposure of school children to indoor air pollutants has increased allergy and respiratory diseases. The objective of this study were to determine the toxicodynamic interaction of indoor pollutants exposure, biological and chemical with expression of adhesion molecules on eosinophil and neutroph...

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Main Authors:	Mohd Isa, Khairul Nizam, Jalaludin, Juliana, Mohd Elias, Saliza, Lung Than, Leslie Thian, Jabbar, Mohammed Abdulrazzaq, Mohd Saudi, Ahmad Shakir, Norbäck, Dan, Hashim, Jamal Hisham, Hashim, Zailina
Format:	Article
Published:	Elsevier 2021
Online Access:	http://psasir.upm.edu.my/id/eprint/94167/ https://www.sciencedirect.com/science/article/pii/S014765132100542X
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spelling	my.upm.eprints.941672023-03-29T01:22:23Z http://psasir.upm.edu.my/id/eprint/94167/ Metagenomic characterization of indoor dust fungal associated with allergy and lung inflammation among school children Mohd Isa, Khairul Nizam Jalaludin, Juliana Mohd Elias, Saliza Lung Than, Leslie Thian Jabbar, Mohammed Abdulrazzaq Mohd Saudi, Ahmad Shakir Norbäck, Dan Hashim, Jamal Hisham Hashim, Zailina The exposure of school children to indoor air pollutants has increased allergy and respiratory diseases. The objective of this study were to determine the toxicodynamic interaction of indoor pollutants exposure, biological and chemical with expression of adhesion molecules on eosinophil and neutrophil. A self-administered questionnaire, allergy skin test, and fractional exhaled nitric oxide (FeNO) analyser were used to collect information on health status, sensitization to allergens and respiratory inflammation, respectively among school children at age of 14 years. The sputum induced were analysed to determine the expression of CD11b, CD35, CD63 and CD66b on eosinophil and neutrophil by using flow cytometry technique. The particulate matter (PM2.5 and PM10), NO2, CO2, and formaldehyde, temperature, and relative humidity were measured inside the classrooms. The fungal DNA were extracted from settled dust collected from classrooms and evaluated using metagenomic techniques. We applied chemometric and regression in statistical analysis. A total of 1869 unique of operational taxonomic units (OTUs) of fungi were identified with dominated at genus level by Aspergillus (15.8%), Verrucoconiothyrium (5.5%), and Ganoderma (4.6%). Chemometric and regression results revealed that relative abundance of T. asahii were associated with down regulation of CD66b expressed on eosinophil, and elevation of FeNO levels in predicting asthmatic children with model accuracy of 63.6%. Meanwhile, upregulation of CD11b expressed on eosinophil were associated with relative abundance of A. clavatus and regulated by PM2.5. There were significant association of P. bandonii with upregulation of CD63 expressed on neutrophil and exposure to NO2. Our findings indicate that exposure to PM2.5, NO2, T. asahii, P.bandonii and A.clavatus are likely interrelated with upregulation of activation and degranulation markers on both eosinophil and neutrophil. Elsevier 2021-09-15 Article PeerReviewed Mohd Isa, Khairul Nizam and Jalaludin, Juliana and Mohd Elias, Saliza and Lung Than, Leslie Thian and Jabbar, Mohammed Abdulrazzaq and Mohd Saudi, Ahmad Shakir and Norbäck, Dan and Hashim, Jamal Hisham and Hashim, Zailina (2021) Metagenomic characterization of indoor dust fungal associated with allergy and lung inflammation among school children. Ecotoxicology and Environmental Safety, 221. pp. 1-13. ISSN 0147-6513; ESSN: 1090-2414 https://www.sciencedirect.com/science/article/pii/S014765132100542X 10.1016/j.ecoenv.2021.112430
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	The exposure of school children to indoor air pollutants has increased allergy and respiratory diseases. The objective of this study were to determine the toxicodynamic interaction of indoor pollutants exposure, biological and chemical with expression of adhesion molecules on eosinophil and neutrophil. A self-administered questionnaire, allergy skin test, and fractional exhaled nitric oxide (FeNO) analyser were used to collect information on health status, sensitization to allergens and respiratory inflammation, respectively among school children at age of 14 years. The sputum induced were analysed to determine the expression of CD11b, CD35, CD63 and CD66b on eosinophil and neutrophil by using flow cytometry technique. The particulate matter (PM2.5 and PM10), NO2, CO2, and formaldehyde, temperature, and relative humidity were measured inside the classrooms. The fungal DNA were extracted from settled dust collected from classrooms and evaluated using metagenomic techniques. We applied chemometric and regression in statistical analysis. A total of 1869 unique of operational taxonomic units (OTUs) of fungi were identified with dominated at genus level by Aspergillus (15.8%), Verrucoconiothyrium (5.5%), and Ganoderma (4.6%). Chemometric and regression results revealed that relative abundance of T. asahii were associated with down regulation of CD66b expressed on eosinophil, and elevation of FeNO levels in predicting asthmatic children with model accuracy of 63.6%. Meanwhile, upregulation of CD11b expressed on eosinophil were associated with relative abundance of A. clavatus and regulated by PM2.5. There were significant association of P. bandonii with upregulation of CD63 expressed on neutrophil and exposure to NO2. Our findings indicate that exposure to PM2.5, NO2, T. asahii, P.bandonii and A.clavatus are likely interrelated with upregulation of activation and degranulation markers on both eosinophil and neutrophil.
format	Article
author	Mohd Isa, Khairul Nizam Jalaludin, Juliana Mohd Elias, Saliza Lung Than, Leslie Thian Jabbar, Mohammed Abdulrazzaq Mohd Saudi, Ahmad Shakir Norbäck, Dan Hashim, Jamal Hisham Hashim, Zailina
spellingShingle	Mohd Isa, Khairul Nizam Jalaludin, Juliana Mohd Elias, Saliza Lung Than, Leslie Thian Jabbar, Mohammed Abdulrazzaq Mohd Saudi, Ahmad Shakir Norbäck, Dan Hashim, Jamal Hisham Hashim, Zailina Metagenomic characterization of indoor dust fungal associated with allergy and lung inflammation among school children
author_facet	Mohd Isa, Khairul Nizam Jalaludin, Juliana Mohd Elias, Saliza Lung Than, Leslie Thian Jabbar, Mohammed Abdulrazzaq Mohd Saudi, Ahmad Shakir Norbäck, Dan Hashim, Jamal Hisham Hashim, Zailina
author_sort	Mohd Isa, Khairul Nizam
title	Metagenomic characterization of indoor dust fungal associated with allergy and lung inflammation among school children
title_short	Metagenomic characterization of indoor dust fungal associated with allergy and lung inflammation among school children
title_full	Metagenomic characterization of indoor dust fungal associated with allergy and lung inflammation among school children
title_fullStr	Metagenomic characterization of indoor dust fungal associated with allergy and lung inflammation among school children
title_full_unstemmed	Metagenomic characterization of indoor dust fungal associated with allergy and lung inflammation among school children
title_sort	metagenomic characterization of indoor dust fungal associated with allergy and lung inflammation among school children
publisher	Elsevier
publishDate	2021
url	http://psasir.upm.edu.my/id/eprint/94167/ https://www.sciencedirect.com/science/article/pii/S014765132100542X
_version_	1761673196748144640
score	13.211869

Metagenomic characterization of indoor dust fungal associated with allergy and lung inflammation among school children

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