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Lipofection of single guide RNA targeting MMP8 decreases proliferation and migration in lung adenocarcinoma cells

Background and Objectives: Matrix metalloproteinases (MMP) have been implicated as major determinants of tumour growth and metastasis, which are considered two of the main hallmarks of cancer. The interaction of MMP8 and other signalling molecules within and adjacent tumoral tissues, including immun...

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Main Authors: Maradiaga, Oscar David Hernandez, Mok, Pooi Ling, Sivapragasam, Gothai, Samrot, Antony V., Ali Khan, Mohammed Safwan, Aisha Farhana, Alzahrani, Badr, Tong, Jiabei, Karuppiah, Thilakavathy, S. Joseph, Narcisse M., Subbiah, Suresh Kumar
Format: Article
Published: MDPI AG 2021
Online Access:http://psasir.upm.edu.my/id/eprint/94040/
https://www.mdpi.com/1648-9144/57/7/710
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spelling my.upm.eprints.940402023-05-18T02:38:09Z http://psasir.upm.edu.my/id/eprint/94040/ Lipofection of single guide RNA targeting MMP8 decreases proliferation and migration in lung adenocarcinoma cells Maradiaga, Oscar David Hernandez Mok, Pooi Ling Sivapragasam, Gothai Samrot, Antony V. Ali Khan, Mohammed Safwan Aisha Farhana Alzahrani, Badr Tong, Jiabei Karuppiah, Thilakavathy S. Joseph, Narcisse M. Subbiah, Suresh Kumar Background and Objectives: Matrix metalloproteinases (MMP) have been implicated as major determinants of tumour growth and metastasis, which are considered two of the main hallmarks of cancer. The interaction of MMP8 and other signalling molecules within and adjacent tumoral tissues, including immune cells, are rather elusive, particularly of adenocarcinoma cell type. In this study, we aimed to investigate the role of MMP8 in non-small cell lung cancer proliferation and invasiveness potential. Materials and Methods: We individually lipofected with two different single guide RNA (sgRNAs) that specifically targeted on MMP8, with CRISPR-Cas 9 protein into the cells. Results: Our results clearly indicated that the lipofection of these complexes could lead to reduced ability of A549 cells to survive and proliferate to form colonies. In addition, when compared to non-transfected cells, the experimental cell groups receiving sgRNAs demonstrated relatively decreased migration rate, hence, wider wound gaps in scratch assay. The quantitative real time-polymerase chain reaction (qRT-PCR) demonstrated significant reduction in the MAP-K, survivin and PI3-K gene expression. MMP8 might have protective roles over tumour growth and spread in our body. Conclusions: The delivery of sgRNAs targeting on the MMP8 gene could induce tumour cell death and arrest cell migratory activity. MDPI AG 2021-07-14 Article PeerReviewed Maradiaga, Oscar David Hernandez and Mok, Pooi Ling and Sivapragasam, Gothai and Samrot, Antony V. and Ali Khan, Mohammed Safwan and Aisha Farhana and Alzahrani, Badr and Tong, Jiabei and Karuppiah, Thilakavathy and S. Joseph, Narcisse M. and Subbiah, Suresh Kumar (2021) Lipofection of single guide RNA targeting MMP8 decreases proliferation and migration in lung adenocarcinoma cells. Medicina-Lithuania, 57 (7). art. no. 710. pp. 1-11. ISSN 1648-9144 https://www.mdpi.com/1648-9144/57/7/710 10.3390/medicina57070710
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Background and Objectives: Matrix metalloproteinases (MMP) have been implicated as major determinants of tumour growth and metastasis, which are considered two of the main hallmarks of cancer. The interaction of MMP8 and other signalling molecules within and adjacent tumoral tissues, including immune cells, are rather elusive, particularly of adenocarcinoma cell type. In this study, we aimed to investigate the role of MMP8 in non-small cell lung cancer proliferation and invasiveness potential. Materials and Methods: We individually lipofected with two different single guide RNA (sgRNAs) that specifically targeted on MMP8, with CRISPR-Cas 9 protein into the cells. Results: Our results clearly indicated that the lipofection of these complexes could lead to reduced ability of A549 cells to survive and proliferate to form colonies. In addition, when compared to non-transfected cells, the experimental cell groups receiving sgRNAs demonstrated relatively decreased migration rate, hence, wider wound gaps in scratch assay. The quantitative real time-polymerase chain reaction (qRT-PCR) demonstrated significant reduction in the MAP-K, survivin and PI3-K gene expression. MMP8 might have protective roles over tumour growth and spread in our body. Conclusions: The delivery of sgRNAs targeting on the MMP8 gene could induce tumour cell death and arrest cell migratory activity.
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author Maradiaga, Oscar David Hernandez
Mok, Pooi Ling
Sivapragasam, Gothai
Samrot, Antony V.
Ali Khan, Mohammed Safwan
Aisha Farhana
Alzahrani, Badr
Tong, Jiabei
Karuppiah, Thilakavathy
S. Joseph, Narcisse M.
Subbiah, Suresh Kumar
spellingShingle Maradiaga, Oscar David Hernandez
Mok, Pooi Ling
Sivapragasam, Gothai
Samrot, Antony V.
Ali Khan, Mohammed Safwan
Aisha Farhana
Alzahrani, Badr
Tong, Jiabei
Karuppiah, Thilakavathy
S. Joseph, Narcisse M.
Subbiah, Suresh Kumar
Lipofection of single guide RNA targeting MMP8 decreases proliferation and migration in lung adenocarcinoma cells
author_facet Maradiaga, Oscar David Hernandez
Mok, Pooi Ling
Sivapragasam, Gothai
Samrot, Antony V.
Ali Khan, Mohammed Safwan
Aisha Farhana
Alzahrani, Badr
Tong, Jiabei
Karuppiah, Thilakavathy
S. Joseph, Narcisse M.
Subbiah, Suresh Kumar
author_sort Maradiaga, Oscar David Hernandez
title Lipofection of single guide RNA targeting MMP8 decreases proliferation and migration in lung adenocarcinoma cells
title_short Lipofection of single guide RNA targeting MMP8 decreases proliferation and migration in lung adenocarcinoma cells
title_full Lipofection of single guide RNA targeting MMP8 decreases proliferation and migration in lung adenocarcinoma cells
title_fullStr Lipofection of single guide RNA targeting MMP8 decreases proliferation and migration in lung adenocarcinoma cells
title_full_unstemmed Lipofection of single guide RNA targeting MMP8 decreases proliferation and migration in lung adenocarcinoma cells
title_sort lipofection of single guide rna targeting mmp8 decreases proliferation and migration in lung adenocarcinoma cells
publisher MDPI AG
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/94040/
https://www.mdpi.com/1648-9144/57/7/710
_version_ 1768009377605746688
score 13.211869

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