Staff View: LEP G2548A polymorphism is associated with increased serum leptin and insulin resistance among T2DM Malaysian patients

LEP G2548A polymorphism is associated with increased serum leptin and insulin resistance among T2DM Malaysian patients

Background: Type 2 diabetes mellitus (T2DM) is a chronic metabolic syndrome that is rapidly increasing across the world, especially in Malaysia. Leptin plays a vital role in the regulation of metabolism through its effect on peripheral tissues. G2548A polymorphism in the LEP gene promoter has been a...

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Main Authors:	Ahmed Ali, Layth, Jemon, Khairunadwa, Ab Latif, Nurriza, Abu Bakar, Suhaili, Syed Alwi, Sharifah Sakinah
Format:	Article
Published:	China Medical University 2021
Online Access:	http://psasir.upm.edu.my/id/eprint/94025/ https://www.biomedicinej.com/biomedicine/vol12/iss3/5/
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spelling	my.upm.eprints.940252023-07-10T00:56:48Z http://psasir.upm.edu.my/id/eprint/94025/ LEP G2548A polymorphism is associated with increased serum leptin and insulin resistance among T2DM Malaysian patients Ahmed Ali, Layth Jemon, Khairunadwa Ab Latif, Nurriza Abu Bakar, Suhaili Syed Alwi, Sharifah Sakinah Background: Type 2 diabetes mellitus (T2DM) is a chronic metabolic syndrome that is rapidly increasing across the world, especially in Malaysia. Leptin plays a vital role in the regulation of metabolism through its effect on peripheral tissues. G2548A polymorphism in the LEP gene promoter has been associated with insulin resistance, leptin, and type 2 diabetes mellitus across different population, but has not been inclusively reported within the Malaysian population. Objective: Thus, our study aimed to investigate the impact of G2548A polymorphism on serum leptin levels and insulin resistance among Malaysian T2DM patients. Methods: This case-control study involved 150 T2DM patients and 150 non-diabetic volunteers from ethnic Malays, Chinese and Indians. Genotyping of G2548A polymorphism was carried out using PCR-RFLP. Serum leptin and insulin levels were determined via ELISA. ANOVA and Chi-square tests were used to determine the distribution of genotypes and allelic frequencies based on serum leptin and insulin levels. Results: Frequency of AA genotype and A allele of G2548A variant were significantly (P < 0.05) higher in T2DM patients of Malay and Indian ethnicities (4%, 35%, and 36%, 57%, respectively) as compared to the control groups (0%, 22%, and 18%, 35%, respectively). Fasting serum leptin levels were significantly (P < 0.001) higher in T2DM patients compared to non-diabetic subjects (166.78 pg/ml, 101.94 pg/ml, respectively). Additionally, elevated serum leptin, insulin levels, and BMI in diabetic patients were found to be associated with the AA genotype of this variant, compared to GG, and GA genotypes (P < 0.05). Conclusion: Our findings suggest a significant association between G2548A polymorphism among Malaysian T2DM subjects, particularly among Malay and Indian ethnic groups. Moreover, the A allele frequency of the G2548A variant significantly increased the risk of T2DM and is significantly associated with increased serum leptin, insulin levels, and elevated BMI. China Medical University 2021 Article PeerReviewed Ahmed Ali, Layth and Jemon, Khairunadwa and Ab Latif, Nurriza and Abu Bakar, Suhaili and Syed Alwi, Sharifah Sakinah (2021) LEP G2548A polymorphism is associated with increased serum leptin and insulin resistance among T2DM Malaysian patients. BioMedicine, 12 (3). pp. 31-39. ISSN 2211-8020 https://www.biomedicinej.com/biomedicine/vol12/iss3/5/ 10.37796/2211-8039.1326
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	Background: Type 2 diabetes mellitus (T2DM) is a chronic metabolic syndrome that is rapidly increasing across the world, especially in Malaysia. Leptin plays a vital role in the regulation of metabolism through its effect on peripheral tissues. G2548A polymorphism in the LEP gene promoter has been associated with insulin resistance, leptin, and type 2 diabetes mellitus across different population, but has not been inclusively reported within the Malaysian population. Objective: Thus, our study aimed to investigate the impact of G2548A polymorphism on serum leptin levels and insulin resistance among Malaysian T2DM patients. Methods: This case-control study involved 150 T2DM patients and 150 non-diabetic volunteers from ethnic Malays, Chinese and Indians. Genotyping of G2548A polymorphism was carried out using PCR-RFLP. Serum leptin and insulin levels were determined via ELISA. ANOVA and Chi-square tests were used to determine the distribution of genotypes and allelic frequencies based on serum leptin and insulin levels. Results: Frequency of AA genotype and A allele of G2548A variant were significantly (P < 0.05) higher in T2DM patients of Malay and Indian ethnicities (4%, 35%, and 36%, 57%, respectively) as compared to the control groups (0%, 22%, and 18%, 35%, respectively). Fasting serum leptin levels were significantly (P < 0.001) higher in T2DM patients compared to non-diabetic subjects (166.78 pg/ml, 101.94 pg/ml, respectively). Additionally, elevated serum leptin, insulin levels, and BMI in diabetic patients were found to be associated with the AA genotype of this variant, compared to GG, and GA genotypes (P < 0.05). Conclusion: Our findings suggest a significant association between G2548A polymorphism among Malaysian T2DM subjects, particularly among Malay and Indian ethnic groups. Moreover, the A allele frequency of the G2548A variant significantly increased the risk of T2DM and is significantly associated with increased serum leptin, insulin levels, and elevated BMI.
format	Article
author	Ahmed Ali, Layth Jemon, Khairunadwa Ab Latif, Nurriza Abu Bakar, Suhaili Syed Alwi, Sharifah Sakinah
spellingShingle	Ahmed Ali, Layth Jemon, Khairunadwa Ab Latif, Nurriza Abu Bakar, Suhaili Syed Alwi, Sharifah Sakinah LEP G2548A polymorphism is associated with increased serum leptin and insulin resistance among T2DM Malaysian patients
author_facet	Ahmed Ali, Layth Jemon, Khairunadwa Ab Latif, Nurriza Abu Bakar, Suhaili Syed Alwi, Sharifah Sakinah
author_sort	Ahmed Ali, Layth
title	LEP G2548A polymorphism is associated with increased serum leptin and insulin resistance among T2DM Malaysian patients
title_short	LEP G2548A polymorphism is associated with increased serum leptin and insulin resistance among T2DM Malaysian patients
title_full	LEP G2548A polymorphism is associated with increased serum leptin and insulin resistance among T2DM Malaysian patients
title_fullStr	LEP G2548A polymorphism is associated with increased serum leptin and insulin resistance among T2DM Malaysian patients
title_full_unstemmed	LEP G2548A polymorphism is associated with increased serum leptin and insulin resistance among T2DM Malaysian patients
title_sort	lep g2548a polymorphism is associated with increased serum leptin and insulin resistance among t2dm malaysian patients
publisher	China Medical University
publishDate	2021
url	http://psasir.upm.edu.my/id/eprint/94025/ https://www.biomedicinej.com/biomedicine/vol12/iss3/5/
_version_	1772813415979417600
score	13.211869

LEP G2548A polymorphism is associated with increased serum leptin and insulin resistance among T2DM Malaysian patients

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