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Isolation, screening and characterization of bacteria associated with cocoa tree roots for different plant growth promotion (PGP) activities

Aims: Plant growth promoting rhizobacteria (PGPR) is a group of bacteria that colonise plant roots and enhance plant growth by a diverse range of mechanisms. This study aims to determine the capabilities of PGPR isolated from cocoa tree roots and their efficiency in enhancing plant growth under gree...

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Main Authors: Madian, Nurfadzilah, Mohd Saud, Halimi, Ahmad, Fisal, Tan, Geok Hun
Format: Article
Published: Malaysian Society for Microbiology 2021
Online Access:http://psasir.upm.edu.my/id/eprint/93933/
http://mjm.usm.my/index.php?r=cms/entry/view&id=83&year=2021
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spelling my.upm.eprints.939332023-04-12T04:12:04Z http://psasir.upm.edu.my/id/eprint/93933/ Isolation, screening and characterization of bacteria associated with cocoa tree roots for different plant growth promotion (PGP) activities Madian, Nurfadzilah Mohd Saud, Halimi Ahmad, Fisal Tan, Geok Hun Aims: Plant growth promoting rhizobacteria (PGPR) is a group of bacteria that colonise plant roots and enhance plant growth by a diverse range of mechanisms. This study aims to determine the capabilities of PGPR isolated from cocoa tree roots and their efficiency in enhancing plant growth under greenhouse conditions. Methodology and results: Eight samples of healthy cocoa tree roots were collected from different locations in Malaysia. Isolated bacteria were screened based on nitrogen fixation, phosphate and potassium solubilization, and catalase activity. The efficiency of purified PGPR was evaluated from pot experiments of cocoa seedlings under greenhouse conditions. Out of 122 isolates, 18 isolates showed several traits of nitrogen fixation, phosphorus and potassium solubilization and were further screened for other plant growth promoting (PGP) traits like catalase and production of indole acetic acid (IAA). Out of all the PGP trait tests, seven isolates showed the most prominent results for in vitro tests and were further tested in vivo for growth promotion of cocoa seedlings under greenhouse conditions. In the presence of bacterial isolates with 2.5 g of inorganic fertilizer, Leclercia adecarboxylata resulted in increases in plant height, leaf number, root length, stem fresh weight and total fresh and dry weight of cocoa seedlings by 15.68%, 17.14%, 9.48%, 5.67%, 11.84% and 25.12%, respectively. Conclusion, significance and impact of study: Based on the result, L. adecarboxylata incorporated with selected carrier material improve cocoa seedling growth and biomass. This formulation also reduces the production cost of inorganic fertilizer and increase the application and development of biofertilizer. Malaysian Society for Microbiology 2021 Article PeerReviewed Madian, Nurfadzilah and Mohd Saud, Halimi and Ahmad, Fisal and Tan, Geok Hun (2021) Isolation, screening and characterization of bacteria associated with cocoa tree roots for different plant growth promotion (PGP) activities. Malaysian Journal of Microbiology, 17 (5). 471 - 481. ISSN 1823-8262; ESSN: 2231-7538 http://mjm.usm.my/index.php?r=cms/entry/view&id=83&year=2021 10.21161/mjm.211097
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Aims: Plant growth promoting rhizobacteria (PGPR) is a group of bacteria that colonise plant roots and enhance plant growth by a diverse range of mechanisms. This study aims to determine the capabilities of PGPR isolated from cocoa tree roots and their efficiency in enhancing plant growth under greenhouse conditions. Methodology and results: Eight samples of healthy cocoa tree roots were collected from different locations in Malaysia. Isolated bacteria were screened based on nitrogen fixation, phosphate and potassium solubilization, and catalase activity. The efficiency of purified PGPR was evaluated from pot experiments of cocoa seedlings under greenhouse conditions. Out of 122 isolates, 18 isolates showed several traits of nitrogen fixation, phosphorus and potassium solubilization and were further screened for other plant growth promoting (PGP) traits like catalase and production of indole acetic acid (IAA). Out of all the PGP trait tests, seven isolates showed the most prominent results for in vitro tests and were further tested in vivo for growth promotion of cocoa seedlings under greenhouse conditions. In the presence of bacterial isolates with 2.5 g of inorganic fertilizer, Leclercia adecarboxylata resulted in increases in plant height, leaf number, root length, stem fresh weight and total fresh and dry weight of cocoa seedlings by 15.68%, 17.14%, 9.48%, 5.67%, 11.84% and 25.12%, respectively. Conclusion, significance and impact of study: Based on the result, L. adecarboxylata incorporated with selected carrier material improve cocoa seedling growth and biomass. This formulation also reduces the production cost of inorganic fertilizer and increase the application and development of biofertilizer.
format Article
author Madian, Nurfadzilah
Mohd Saud, Halimi
Ahmad, Fisal
Tan, Geok Hun
spellingShingle Madian, Nurfadzilah
Mohd Saud, Halimi
Ahmad, Fisal
Tan, Geok Hun
Isolation, screening and characterization of bacteria associated with cocoa tree roots for different plant growth promotion (PGP) activities
author_facet Madian, Nurfadzilah
Mohd Saud, Halimi
Ahmad, Fisal
Tan, Geok Hun
author_sort Madian, Nurfadzilah
title Isolation, screening and characterization of bacteria associated with cocoa tree roots for different plant growth promotion (PGP) activities
title_short Isolation, screening and characterization of bacteria associated with cocoa tree roots for different plant growth promotion (PGP) activities
title_full Isolation, screening and characterization of bacteria associated with cocoa tree roots for different plant growth promotion (PGP) activities
title_fullStr Isolation, screening and characterization of bacteria associated with cocoa tree roots for different plant growth promotion (PGP) activities
title_full_unstemmed Isolation, screening and characterization of bacteria associated with cocoa tree roots for different plant growth promotion (PGP) activities
title_sort isolation, screening and characterization of bacteria associated with cocoa tree roots for different plant growth promotion (pgp) activities
publisher Malaysian Society for Microbiology
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/93933/
http://mjm.usm.my/index.php?r=cms/entry/view&id=83&year=2021
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score 13.211869

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