Lipase-Catalyzed Synthesis of Wax Ester
Alcoholysis reaction between triolein and oleyl alcohol catalyzed by Lipozyme and Novozyme was carried out to produce oleyl oleate, a wax ester. Several reaction parameters were studied in order to get its optimal condition such as effect of time, reaction temperature, amount of enzyme, molar rat...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2001
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/9321/1/FSAS_2001_54.pdf http://psasir.upm.edu.my/id/eprint/9321/ |
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| Summary: | Alcoholysis reaction between triolein and oleyl alcohol catalyzed by Lipozyme
and Novozyme was carried out to produce oleyl oleate, a wax ester. Several reaction
parameters were studied in order to get its optimal condition such as effect of time,
reaction temperature, amount of enzyme, molar ratio of substrates (oleyl alcohol/trioleib),
various organic solvents used, initial water activity (aw), continuously controlled water
activity and addition of silica gel.
The optimal condition to produce wax ester for Lipozyme are incubation time of
5 h, temperature of 50°c and using 30% (w/v) of Lipozyme. The molar ratio of substmte
was 6:1 (oleyl alcohol/triolein) in heptane and at aw 0.33 (initial water activity) or 0.32
(continuously controlled water activity) with the addition of 0.25 g of silica gel. For
Novozyme, the optimum incubation time was 5 h, at reaction temperature of 60 °c using
30% (w/v) of Novozyme. The optimum molar ratio of substrates (oleyl alcohol/triolein) was also at 6:1, in heptane at aw 0.33 (initial water activity, aw), 0.32 (continuously
controlled water activity) and with the addition of 0.50 g of silica gel.
A scale up reaction was then carried out using the optimum condition catalyzed
by Lipozyme. The percentage yield of wax ester produced at optimum reaction condition
was 58.48% when determined by gravimetry method and 75.66% when determined using
gas chromatography. |
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