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Cryopreservation of Dendrobium shavin white using sucrose preculture - encapsulation vitrification method

Dendrobium Shavin White is a hybrid from the crossing between Dendrobium Walter Oumae and Dendrobium Queen Florist which has vigorous growth and durable. Dendrobium Shavin White produces flowers all year around with high number of flowers in a single inflorescence. The bloom is usually greenish w...

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Main Author: Nagappan, Pugalanthi
Format: Project Paper Report
Language:English
Published: 2015
Online Access:http://psasir.upm.edu.my/id/eprint/91601/1/FP%202015%20101%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/91601/
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spelling my.upm.eprints.916012021-12-01T03:55:35Z http://psasir.upm.edu.my/id/eprint/91601/ Cryopreservation of Dendrobium shavin white using sucrose preculture - encapsulation vitrification method Nagappan, Pugalanthi Dendrobium Shavin White is a hybrid from the crossing between Dendrobium Walter Oumae and Dendrobium Queen Florist which has vigorous growth and durable. Dendrobium Shavin White produces flowers all year around with high number of flowers in a single inflorescence. The bloom is usually greenish white. PLB, the somatic embryo of orchid is one of the tissues with high ability to become plantlets when cultured. Growth and development of PLBs cannot be arrested due to active cellular divisions. Therefore a suitable protocol to preserve the PLBs is required for efficient handling of the planting material. This experiment is conducted to determine the survival of naked PLB exposure to different concentrations of sucrose, followed by exposure to PVS2 after encapsulation upon cryopreservation. It is also conducted to determine the survival of encapsulated PLB exposure to different concentrations of sucrose, followed by exposure to PVS2 upon cryopreservation. Prior to cryopreservation of PLBs, viability test was carried out. The experimental design used was CRD with 4 replications. Data collected were percentages of PLBs regeneration and colour of PLBs. Data show that, the highest survival percentage of PLBs in LN was recorded by encapsulated PLB pre treated with 0.2 M sucrose concentration in 15 minutes soaking of PVS2 (31.25%). 2015 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/91601/1/FP%202015%20101%20-%20IR.pdf Nagappan, Pugalanthi (2015) Cryopreservation of Dendrobium shavin white using sucrose preculture - encapsulation vitrification method. [Project Paper Report]
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Dendrobium Shavin White is a hybrid from the crossing between Dendrobium Walter Oumae and Dendrobium Queen Florist which has vigorous growth and durable. Dendrobium Shavin White produces flowers all year around with high number of flowers in a single inflorescence. The bloom is usually greenish white. PLB, the somatic embryo of orchid is one of the tissues with high ability to become plantlets when cultured. Growth and development of PLBs cannot be arrested due to active cellular divisions. Therefore a suitable protocol to preserve the PLBs is required for efficient handling of the planting material. This experiment is conducted to determine the survival of naked PLB exposure to different concentrations of sucrose, followed by exposure to PVS2 after encapsulation upon cryopreservation. It is also conducted to determine the survival of encapsulated PLB exposure to different concentrations of sucrose, followed by exposure to PVS2 upon cryopreservation. Prior to cryopreservation of PLBs, viability test was carried out. The experimental design used was CRD with 4 replications. Data collected were percentages of PLBs regeneration and colour of PLBs. Data show that, the highest survival percentage of PLBs in LN was recorded by encapsulated PLB pre treated with 0.2 M sucrose concentration in 15 minutes soaking of PVS2 (31.25%).
format Project Paper Report
author Nagappan, Pugalanthi
spellingShingle Nagappan, Pugalanthi
Cryopreservation of Dendrobium shavin white using sucrose preculture - encapsulation vitrification method
author_facet Nagappan, Pugalanthi
author_sort Nagappan, Pugalanthi
title Cryopreservation of Dendrobium shavin white using sucrose preculture - encapsulation vitrification method
title_short Cryopreservation of Dendrobium shavin white using sucrose preculture - encapsulation vitrification method
title_full Cryopreservation of Dendrobium shavin white using sucrose preculture - encapsulation vitrification method
title_fullStr Cryopreservation of Dendrobium shavin white using sucrose preculture - encapsulation vitrification method
title_full_unstemmed Cryopreservation of Dendrobium shavin white using sucrose preculture - encapsulation vitrification method
title_sort cryopreservation of dendrobium shavin white using sucrose preculture - encapsulation vitrification method
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/91601/1/FP%202015%20101%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/91601/
_version_ 1718927779202859008
score 13.211869

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