Cover Image

In vitro catharanthus roseus (L.) G. don callus responses on dark and chitosan treatments

Catharanthus roseus (L.) G. Don or periwinkle which originates from Madagascar belongs to the family Apocynaceae. The species has been thoroughly investigated to have alkaloids routinely used in the treatment of various cancer diseases. The present study was conducted to observe the effect of variou...

Full description

Saved in:
Bibliographic Details
Main Author: Sulaiman, Nur Amani Azwa
Format: Project Paper Report
Language:English
Published: 2015
Online Access:http://psasir.upm.edu.my/id/eprint/91586/1/FP%202015%2085%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/91586/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.91586
record_format eprints
spelling my.upm.eprints.915862021-12-01T04:06:10Z http://psasir.upm.edu.my/id/eprint/91586/ In vitro catharanthus roseus (L.) G. don callus responses on dark and chitosan treatments Sulaiman, Nur Amani Azwa Catharanthus roseus (L.) G. Don or periwinkle which originates from Madagascar belongs to the family Apocynaceae. The species has been thoroughly investigated to have alkaloids routinely used in the treatment of various cancer diseases. The present study was conducted to observe the effect of various concentrations of chitosan in the dark on callus growth and development. Callus of C. roseus was used as explants. The callus which was initially initiated on Murashige and Skoog medium was cultured on same medium supplemented with various levels of chitosan (0, 1, 2, 3, 4, 5 mg/L). The experiment was conducted in a Complete Randomized Design (CRD) with 3 replications. Callus growth and development under dark treatment were monitored for a duration of 8 weeks. The main objective of the study was to observe responses on growth and development of callus of C. roseus on chitosan treatments. Data recorded that 3 mg/L chitosan gave the highest mean dry weight 0.28 g while the lowest was 0.17 g from 4 mg/L treatment. The control gave the highest level of boron of 0.18% while in 5 mg/L treatment gave the lowest level of 0.05%. The study concludes that callus growth decreases on medium supplemented with chitosan. Dark treatment did not give positive responses to chitosan supplementation. 2015 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/91586/1/FP%202015%2085%20-%20IR.pdf Sulaiman, Nur Amani Azwa (2015) In vitro catharanthus roseus (L.) G. don callus responses on dark and chitosan treatments. [Project Paper Report]
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Catharanthus roseus (L.) G. Don or periwinkle which originates from Madagascar belongs to the family Apocynaceae. The species has been thoroughly investigated to have alkaloids routinely used in the treatment of various cancer diseases. The present study was conducted to observe the effect of various concentrations of chitosan in the dark on callus growth and development. Callus of C. roseus was used as explants. The callus which was initially initiated on Murashige and Skoog medium was cultured on same medium supplemented with various levels of chitosan (0, 1, 2, 3, 4, 5 mg/L). The experiment was conducted in a Complete Randomized Design (CRD) with 3 replications. Callus growth and development under dark treatment were monitored for a duration of 8 weeks. The main objective of the study was to observe responses on growth and development of callus of C. roseus on chitosan treatments. Data recorded that 3 mg/L chitosan gave the highest mean dry weight 0.28 g while the lowest was 0.17 g from 4 mg/L treatment. The control gave the highest level of boron of 0.18% while in 5 mg/L treatment gave the lowest level of 0.05%. The study concludes that callus growth decreases on medium supplemented with chitosan. Dark treatment did not give positive responses to chitosan supplementation.
format Project Paper Report
author Sulaiman, Nur Amani Azwa
spellingShingle Sulaiman, Nur Amani Azwa
In vitro catharanthus roseus (L.) G. don callus responses on dark and chitosan treatments
author_facet Sulaiman, Nur Amani Azwa
author_sort Sulaiman, Nur Amani Azwa
title In vitro catharanthus roseus (L.) G. don callus responses on dark and chitosan treatments
title_short In vitro catharanthus roseus (L.) G. don callus responses on dark and chitosan treatments
title_full In vitro catharanthus roseus (L.) G. don callus responses on dark and chitosan treatments
title_fullStr In vitro catharanthus roseus (L.) G. don callus responses on dark and chitosan treatments
title_full_unstemmed In vitro catharanthus roseus (L.) G. don callus responses on dark and chitosan treatments
title_sort in vitro catharanthus roseus (l.) g. don callus responses on dark and chitosan treatments
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/91586/1/FP%202015%2085%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/91586/
_version_ 1718927776914866176
score 13.211869

Similar Items