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Identification of a putative monolignol transporter gene homolog II in Oryza sativa

Plant cells have sturdy shape because of the presence of cell wall made from three components; cellulose, hemicelluloses, and lignin. Lignin biosynthesis has been studied extensively by many researchers but monolignol transportation process is yet to be explored specifically. It is suggested that...

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Main Author: Ramizan, Muhammad Assiddiq
Format: Project Paper Report
Language:English
Published: 2015
Online Access:http://psasir.upm.edu.my/id/eprint/91043/1/FBSB%202015%20153%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/91043/
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spelling my.upm.eprints.910432021-10-26T00:50:47Z http://psasir.upm.edu.my/id/eprint/91043/ Identification of a putative monolignol transporter gene homolog II in Oryza sativa Ramizan, Muhammad Assiddiq Plant cells have sturdy shape because of the presence of cell wall made from three components; cellulose, hemicelluloses, and lignin. Lignin biosynthesis has been studied extensively by many researchers but monolignol transportation process is yet to be explored specifically. It is suggested that monolignol uptakes are dependent towards transport proteins residing in the membrane. AtABCG29 gene of Arabidopsis thaliana codes for a transport protein has been characterized and proven that the protein transports p-coumaryl alcohol. However, the monolignol transport mechanism for Oryza sativa is still elusive. In this study, Oryza sativa‘s gene with locus name OsI_03578 was identified as a putative homologous gene of AtABCG29. OsI_03578 was chosen due to its high sequence similarity with AtABCG29. This research was conducted to investigate whether OsI_03578 gene encodes for a protein that involves in monolignol transportation in Oryza sativa. Total RNA was extracted from Oryza sativa and used to synthesize cDNA which served as a template to amplify OsI_03578 sequence in Oryza sativa. The amplification of the gene sequence was performed by PCR using different sets of primers. In this project, conventional RNA extraction protocol has been optimized and proven to give better RNA yield compared to RNA extraction using commercial kit. However, designation of new primers is necessary in order to amplify specific target region in the gene. 2015-06 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/91043/1/FBSB%202015%20153%20-%20IR.pdf Ramizan, Muhammad Assiddiq (2015) Identification of a putative monolignol transporter gene homolog II in Oryza sativa. [Project Paper Report]
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Plant cells have sturdy shape because of the presence of cell wall made from three components; cellulose, hemicelluloses, and lignin. Lignin biosynthesis has been studied extensively by many researchers but monolignol transportation process is yet to be explored specifically. It is suggested that monolignol uptakes are dependent towards transport proteins residing in the membrane. AtABCG29 gene of Arabidopsis thaliana codes for a transport protein has been characterized and proven that the protein transports p-coumaryl alcohol. However, the monolignol transport mechanism for Oryza sativa is still elusive. In this study, Oryza sativa‘s gene with locus name OsI_03578 was identified as a putative homologous gene of AtABCG29. OsI_03578 was chosen due to its high sequence similarity with AtABCG29. This research was conducted to investigate whether OsI_03578 gene encodes for a protein that involves in monolignol transportation in Oryza sativa. Total RNA was extracted from Oryza sativa and used to synthesize cDNA which served as a template to amplify OsI_03578 sequence in Oryza sativa. The amplification of the gene sequence was performed by PCR using different sets of primers. In this project, conventional RNA extraction protocol has been optimized and proven to give better RNA yield compared to RNA extraction using commercial kit. However, designation of new primers is necessary in order to amplify specific target region in the gene.
format Project Paper Report
author Ramizan, Muhammad Assiddiq
spellingShingle Ramizan, Muhammad Assiddiq
Identification of a putative monolignol transporter gene homolog II in Oryza sativa
author_facet Ramizan, Muhammad Assiddiq
author_sort Ramizan, Muhammad Assiddiq
title Identification of a putative monolignol transporter gene homolog II in Oryza sativa
title_short Identification of a putative monolignol transporter gene homolog II in Oryza sativa
title_full Identification of a putative monolignol transporter gene homolog II in Oryza sativa
title_fullStr Identification of a putative monolignol transporter gene homolog II in Oryza sativa
title_full_unstemmed Identification of a putative monolignol transporter gene homolog II in Oryza sativa
title_sort identification of a putative monolignol transporter gene homolog ii in oryza sativa
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/91043/1/FBSB%202015%20153%20-%20IR.pdf
http://psasir.upm.edu.my/id/eprint/91043/
_version_ 1715191599599714304
score 13.211869

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