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Review on the in vitro cytotoxicity assessment in accordance to the International Organization for Standardization (ISO)

Cytotoxicity is a predominant biological evaluation applied to search for a suitable and non-toxic bioactive compound and to determine the biocompatibility of medical devices-related human body. The broad usage of cytotoxicity tests leads to a robust establishment of cytotoxicity assays with high se...

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Main Authors: Mohd Shafiee, Muhammad Aminuddin, Muhamad Asri, Mohd Ashraf, Syed Alwi, Sharifah Sakinah
Format: Article
Language:English
Published: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2021
Online Access:http://psasir.upm.edu.my/id/eprint/90357/1/2021040613505636_MJMHS_0637.pdf
http://psasir.upm.edu.my/id/eprint/90357/
https://medic.upm.edu.my/upload/dokumen/2021040613505636_MJMHS_0637.pdf
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spelling my.upm.eprints.903572021-09-10T01:00:21Z http://psasir.upm.edu.my/id/eprint/90357/ Review on the in vitro cytotoxicity assessment in accordance to the International Organization for Standardization (ISO) Mohd Shafiee, Muhammad Aminuddin Muhamad Asri, Mohd Ashraf Syed Alwi, Sharifah Sakinah Cytotoxicity is a predominant biological evaluation applied to search for a suitable and non-toxic bioactive compound and to determine the biocompatibility of medical devices-related human body. The broad usage of cytotoxicity tests leads to a robust establishment of cytotoxicity assays with high sensitivity and prompt results. In vitro assays are always prioritized over in vivo due to the reproducible data, reduce numbers of animal used and easily accessible material. Compounds concentration that execute 50% of cell population is determined by calculating the IC50. According to ISO10993, cytotoxicity tests must be performed to determine the biocompatibility of medical devices that has contact with human body. This is crucial to ensure the safety of research and its clinical use. Under the recommendation of ISO10995-Part 5, three categories of tests have been documented; extract elution, direct contact and indirect contact test. Each category plays significant role depending on the nature of experiment and sample used. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2021-04 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/90357/1/2021040613505636_MJMHS_0637.pdf Mohd Shafiee, Muhammad Aminuddin and Muhamad Asri, Mohd Ashraf and Syed Alwi, Sharifah Sakinah (2021) Review on the in vitro cytotoxicity assessment in accordance to the International Organization for Standardization (ISO). Malaysian Journal of Medicine and Health Sciences, 17 (2). pp. 261-269. ISSN 1675-8544; ESSN: 2636-9346 https://medic.upm.edu.my/upload/dokumen/2021040613505636_MJMHS_0637.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Cytotoxicity is a predominant biological evaluation applied to search for a suitable and non-toxic bioactive compound and to determine the biocompatibility of medical devices-related human body. The broad usage of cytotoxicity tests leads to a robust establishment of cytotoxicity assays with high sensitivity and prompt results. In vitro assays are always prioritized over in vivo due to the reproducible data, reduce numbers of animal used and easily accessible material. Compounds concentration that execute 50% of cell population is determined by calculating the IC50. According to ISO10993, cytotoxicity tests must be performed to determine the biocompatibility of medical devices that has contact with human body. This is crucial to ensure the safety of research and its clinical use. Under the recommendation of ISO10995-Part 5, three categories of tests have been documented; extract elution, direct contact and indirect contact test. Each category plays significant role depending on the nature of experiment and sample used.
format Article
author Mohd Shafiee, Muhammad Aminuddin
Muhamad Asri, Mohd Ashraf
Syed Alwi, Sharifah Sakinah
spellingShingle Mohd Shafiee, Muhammad Aminuddin
Muhamad Asri, Mohd Ashraf
Syed Alwi, Sharifah Sakinah
Review on the in vitro cytotoxicity assessment in accordance to the International Organization for Standardization (ISO)
author_facet Mohd Shafiee, Muhammad Aminuddin
Muhamad Asri, Mohd Ashraf
Syed Alwi, Sharifah Sakinah
author_sort Mohd Shafiee, Muhammad Aminuddin
title Review on the in vitro cytotoxicity assessment in accordance to the International Organization for Standardization (ISO)
title_short Review on the in vitro cytotoxicity assessment in accordance to the International Organization for Standardization (ISO)
title_full Review on the in vitro cytotoxicity assessment in accordance to the International Organization for Standardization (ISO)
title_fullStr Review on the in vitro cytotoxicity assessment in accordance to the International Organization for Standardization (ISO)
title_full_unstemmed Review on the in vitro cytotoxicity assessment in accordance to the International Organization for Standardization (ISO)
title_sort review on the in vitro cytotoxicity assessment in accordance to the international organization for standardization (iso)
publisher Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/90357/1/2021040613505636_MJMHS_0637.pdf
http://psasir.upm.edu.my/id/eprint/90357/
https://medic.upm.edu.my/upload/dokumen/2021040613505636_MJMHS_0637.pdf
_version_ 1712286776084660224
score 13.211869

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